Publication Information

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3705, 3809, 3813, 3830, 3883, 3895, 3932, 3975, 4066, 4081, 4097, 4161, 4203, 4213, 4217], 'series': [23, 196], 'of': [24, 35, 52, 90, 151, 159, 165, 197, 208, 225, 263, 324, 332, 338, 349, 360, 368, 399, 408, 495, 515, 528, 553, 616, 621, 759, 792, 795, 825, 832, 888, 929, 972, 1067, 1069, 1104, 1111, 1161, 1170, 1192, 1210, 1222, 1232, 1278, 1303, 1316, 1330, 1489, 1533, 1577, 1589, 1601, 1670, 1674, 1700, 1726, 1748, 1845, 1916, 1962, 2005, 2054, 2057, 2115, 2132, 2144, 2151, 2160, 2164, 2171, 2179, 2227, 2250, 2254, 2268, 2287, 2300, 2311, 2329, 2342, 2358, 2369, 2552, 2583, 2591, 2605, 2612, 2655, 2658, 2693, 2719, 2722, 2731, 2735, 2741, 2752, 2760, 2783, 2793, 2798, 2872, 2877, 2938, 3018, 3023, 3068, 3116, 3173, 3176, 3199, 3205, 3246, 3279, 3318, 3321, 3349, 3361, 3370, 3372, 3398, 3404, 3419, 3439, 3462, 3506, 3567, 3577, 3591, 3600, 3713, 3748, 3756, 3808, 3826, 3833, 3878, 3886, 3950, 3980, 3985, 3988, 4021, 4039, 4062, 4084, 4088, 4163, 4167, 4205], 'mutant': [25, 54, 70, 198, 227, 243, 917, 1242, 1524, 2101, 2213, 2218, 2492, 2553, 2649, 2701, 2886, 2941, 3182, 3272, 3284, 3915, 4100, 4228], 'GSTUs': [26, 55, 199, 228, 782, 918, 2007, 2451, 2804, 3465, 3989], 'were': [27, 200, 953, 989, 1115, 1165, 1214, 1238, 1260, 1293, 1325, 1344, 1354, 1386, 1452, 1478, 1558, 1730, 1787, 1817, 1825, 1869, 1880, 1925, 1975, 2046, 2158, 2191, 2221, 2231, 2318, 2375, 2392, 2421, 2467, 2633, 3072, 3309, 3332, 3383, 3456, 3547, 3606, 3677, 3686, 3860, 3917, 3928, 3942, 3961, 4057], 'generated': [28, 93, 201, 266, 1703, 2549, 3415, 3434], 'from': [29, 202, 453, 480, 920, 932, 991, 1246, 1691, 1736, 1887, 2082, 2243, 2260, 2272, 2648, 2660, 3037, 3603, 3990, 4106, 4112, 4122], 'vitro': [31, 204, 2073, 3545], 'recombination': [32, 205], 'mutagenesis': [34, 89, 207, 262, 944, 2606, 2792, 2920, 3167, 3204], 'the': [36, 43, 77, 107, 121, 126, 138, 142, 149, 157, 209, 216, 250, 280, 294, 299, 311, 315, 322, 330, 400, 491, 526, 532, 614, 617, 627, 707, 756, 789, 798, 822, 829, 847, 857, 886, 930, 935, 1027, 1042, 1070, 1098, 1211, 1263, 1273, 1301, 1317, 1321, 1350, 1409, 1413, 1528, 1548, 1554, 1567, 1599, 1644, 1656, 1671, 1692, 1719, 1737, 1743, 1749, 1831, 1960, 2108, 2113, 2126, 2148, 2161, 2167, 2176, 2180, 2217, 2228, 2248, 2252, 2255, 2284, 2288, 2298, 2322, 2327, 2350, 2356, 2367, 2380, 2414, 2424, 2446, 2471, 2475, 2483, 2491, 2506, 2581, 2592, 2603, 2613, 2635, 2639, 2661, 2684, 2691, 2713, 2717, 2720, 2732, 2738, 2747, 2753, 2769, 2779, 2790, 2806, 2816, 2878, 2885, 2892, 2899, 2925, 2930, 2936, 2994, 3015, 3048, 3054, 3092, 3104, 3153, 3171, 3197, 3221, 3231, 3250, 3265, 3276, 3282, 3300, 3339, 3388, 3396, 3399, 3411, 3428, 3440, 3460, 3463, 3474, 3503, 3533, 3541, 3554, 3562, 3574, 3578, 3722, 3823, 3879, 3913, 3921, 3926, 3935, 3946, 4026, 4042, 4054, 4074, 4085, 4094, 4129, 4144, 4164, 4168, 4235], 'maize': [37, 210, 502, 593, 839], 'sequences': [38, 211, 983, 1729], 'ZmGSTU1': [39, 212, 843, 922, 985, 1033, 2165, 2208, 2244, 2273, 2281, 2528, 2736, 3019, 3938, 4040, 4077, 4113], 'ZmGSTU2': [41, 214, 845, 1038, 1594, 2742, 3084, 4022, 4035, 4089, 4107, 4123, 4169], '(with': [42, 215], 'prefix': [44, 217], 'Zm': [45, 218], 'designating': [46, 219], 'Zea': [47, 220], 'mays': [48, 221, 455, 482, 841], 'L.).': [49, 222], 'A': [50, 223, 1697, 2091, 2120], 'screen': [51, 224], '5000': [53, 226, 2100], 'identified': [56, 229, 2192], 'seven': [57, 230, 2189, 2229, 2315], 'enzymes': [58, 231, 696, 709, 850, 2351, 2493, 2554, 2650], 'with': [59, 80, 96, 106, 110, 156, 232, 253, 269, 279, 283, 329, 392, 531, 784, 1077, 1097, 1101, 1236, 1240, 1476, 1544, 1682, 1772, 1855, 1967, 1977, 1995, 2125, 2166, 2302, 2355, 2377, 2486, 2490, 2531, 2537, 2677, 2683, 2688, 2908, 2935, 2942, 2965, 3047, 3127, 3142, 3170, 3229, 3255, 3338, 3509, 3550, 3670, 3680, 3752, 3763, 3890, 3920, 3945, 4036, 4059, 4073, 4118, 4153, 4171, 4216], 'enhanced': [60, 67, 130, 233, 240, 303, 448, 475, 2104, 2136, 3105, 3537, 3806, 4232], 'fluorodifen': [61, 68, 103, 234, 241, 276, 449, 476, 578, 1287, 1400, 1451, 1522, 1701, 1945, 1980, 2110, 2184, 2378, 2466, 2538, 2561, 2808, 2947, 3106, 3192, 3218, 3431, 3481, 3528, 3587, 3650, 3683, 3749, 3867, 3893, 3922, 4147, 4172, 4185, 4223], 'detoxifying': [62, 69, 235, 242, 450, 477, 714, 823, 891, 1523, 2781, 3215, 3413, 3432, 3529, 3580, 3810], 'activity.': [63, 236, 948, 2371, 3108], 'The': [64, 237, 346, 677, 949, 1258, 1486, 1649, 1668, 1784, 2340, 2372, 2462, 2956, 3146, 3161, 3180, 3486, 3646, 3746, 4103], 'best': [65, 238], 'performing': [66, 239], '(EFD)': [71, 244], 'had': [72, 245, 2194, 2548, 2556, 2620, 2667, 2702, 2762, 3043, 3080, 3184, 3209, 3970], 'activity': [73, 246, 892, 1395, 1446, 1931, 2106, 2178, 2200, 2511, 2934, 2945, 3046, 3129, 3216, 3483, 3530, 3539, 3560, 3582, 3825], '19-fold': [74, 247, 3557], 'higher': [75, 99, 248, 272, 2142, 2198, 2468], 'than': [76, 249, 856, 1320, 1450, 2204, 2534, 2712, 3158, 3561, 3720], 'parent': [78, 127, 251, 300, 1322, 2127, 2450, 2476, 2508, 2640, 3224, 3266, 3389, 4076], 'enzymes,': [79, 128, 252, 301, 1605], 'single': [82, 255, 2076, 2293], 'point': [83, 256, 1517, 3093], 'mutation': [84, 140, 257, 313, 1518, 2295, 2958, 3094, 3115], 'conferring': [85, 258], 'this': [86, 91, 259, 264, 785, 933, 1171, 2700, 2794, 2905, 2909, 3271, 3507, 3510, 4227, 4240], 'enhancement.': [87, 260], 'Further': [88, 261], 'residue': [92, 265, 1532, 1675, 2305, 2309, 2723, 2772, 2927, 2972, 3098, 3117, 3174, 3207, 4131], 'an': [94, 152, 267, 325, 1897, 3125, 3253, 3470, 3614, 3871], 'EFD': [95, 268, 2212, 2316, 2344, 3373, 4027, 4099], '29-fold': [98, 271, 3212], 'catalytic': [100, 273, 2435], 'efficiency': [101, 274, 2436], 'toward': [102, 113, 275, 286, 701, 893, 1396, 1447, 2107, 2183, 2417, 2512, 2524, 2560, 2564, 2624, 2671, 2805, 2946, 3085, 3130, 3134, 3155, 3191, 3217, 3240, 3249, 3260, 3292, 3540, 3649, 4069], 'compared': [105, 278, 2124, 2530, 3228, 3762, 4072], 'parents': [108, 281], 'but': [109, 124, 282, 297, 589, 2290, 2562, 2979, 3897], 'unaltered': [111, 284], 'catalysis': [112, 285], 'other': [114, 287, 563, 1449, 2714, 2754, 2900, 4075], 'substrates.': [115, 288], 'When': [116, 289, 3299, 3525], 'expressed': [117, 290, 760, 2890, 3027, 4243], 'Arabidopsis': [119, 292, 958, 3381, 3858, 3888], 'thaliana,': [120, 293], 'optimized': [122, 295, 951, 3412, 3914], 'EFD,': [123, 296, 447, 474], 'not': [125, 298, 2362, 2563, 2576, 3274, 3573], 'conferred': [129, 302], 'tolerance': [131, 304, 968, 3585, 3821], 'to': [132, 148, 170, 305, 321, 343, 536, 914, 942, 945, 956, 965, 1255, 1271, 1389, 1527, 1537, 1547, 1563, 1580, 1592, 1640, 1796, 1819, 1853, 1863, 1970, 2017, 2098, 2140, 2297, 2308, 2366, 2401, 2411, 2474, 2482, 2504, 2527, 2578, 2601, 2923, 2976, 3008, 3010, 3074, 3083, 3119, 3121, 3178, 3194, 3220, 3264, 3335, 3377, 3386, 3473, 3502, 3569, 3586, 3822, 3862, 3866, 3972, 3978, 4030, 4092, 4157, 4181, 4195, 4221], 'fluorodifen.': [133, 306, 1439, 2461, 3298, 4031], 'Molecular': [134, 307], 'modeling': [135, 308, 4020], 'predicts': [136, 309], 'that': [137, 310, 1474, 2156, 2193, 2544, 2555, 2632, 2651, 2699, 2768, 2884, 3091, 3270, 3805, 3882, 3979, 4111, 4120, 4226], 'serendipitous': [139, 312], 'giving': [141, 314, 2993, 3252], 'improvement': [143, 316], 'detoxification': [145, 318, 351, 423, 607, 791, 3757], 'is': [146, 319, 356, 3750, 3829, 4108, 4114, 4133, 4138, 4148], 'due': [147, 320, 2139, 2365, 2400, 3501], 'removal': [150, 323], 'unfavorable': [153, 326], 'interaction': [154, 327], 'together': [155, 328, 1543, 2682], 'introduction': [158, 331], 'favorable': [161, 334], 'change': [162, 335, 1048, 2707, 3148], 'conformation': [164, 337, 1669, 1715], 'residues': [166, 339], '107–119,': [167, 340], 'which': [168, 341, 2360, 2420, 2758, 3071, 3499, 3828, 3900], 'contribute': [169, 342], 'herbicide': [171, 344, 350, 569, 687, 796, 967, 2109, 3542, 3579, 3708, 4146, 4241], 'binding.': [172, 345], 'relative': [347, 2473, 2503, 2526, 3193, 3219], 'rate': [348, 3755, 3976], 'weeds': [355], 'primary': [358], 'determinant': [359], '(1Owen': [363, 403], 'W.J.': [364, 404], 'Roberts': [365, 405], 'T.': [366, 406], 'Metabolism': [367, 407], 'Agrochemicals': [369, 409], 'Plants.': [371, 411], 'John': [372, 412], 'Wiley': [373, 413], 'Sons': [375, 415], 'Ltd.,': [376, 416], 'London2000:': [377, 417], '211-258Google': [378, 418], 'Scholar).': [379, 419, 523, 561, 604, 676, 751, 884, 1025, 1061, 1132, 1383, 1766, 1814, 1912, 3524, 3745, 3799, 3857], 'Crops': [380], 'rapidly': [381, 580], 'metabolize': [382], 'by': [384, 390, 582, 611, 1268, 1411, 1734, 1802, 1891, 1935, 1965, 2008, 2080, 2263, 2338, 2352, 2570, 3111, 3151, 3435, 3466, 3478, 3608, 4239], 'oxidative': [385], 'or': [386, 394, 1338, 2209, 2266, 2497, 2989, 3041, 3443, 3572, 3675, 3685, 3912], 'hydrolytic': [387], 'reactions': [388, 424], 'followed': [389], 'conjugation': [391, 527], 'sugars': [393], 'peptides': [395], 'vacuolar': [397], 'sequestration': [398], 'polar': [401], 'products': [402, 540, 1160, 1209, 1557], 'In': [420, 562, 752, 838, 1026, 2173, 2276, 2664, 3057], 'weeds,': [421, 3765], 'these': [422, 529, 2418, 2695, 3024], 'are': [425, 579, 683, 698, 711, 755, 846, 2652, 3656, 4033, 4187], 'slower.': [426], 'Glutathione': [427, 4137], 'S-transferases': [428], '(GSTs)': [429], '1The': [430, 457], 'abbreviations': [431, 458], 'used': [432, 459, 897, 955, 1116, 1174, 1795, 2097, 2393], 'are:': [433, 460], 'GST,': [434, 461, 4214], 'S-transferase;': [436, 463], 'GSTF,': [437, 464], 'Phi': [438, 465, 679, 695], 'GST;': [440, 444, 467, 471], 'GSTU,': [441, 468], 'CDNB,': [445, 472, 2820, 3089, 3241], '1-chloro-2,4-dinitrobenzene;': [446, 473], 'mutant;': [451, 478], 'Zm,': [452, 479], 'Z.': [454, 481], 'L.': [456, 483], 'have': [484, 817, 896, 3275, 4211], 'well': [486, 2422, 2822, 4024], 'characterized': [487], 'role': [488], 'metabolism': [492, 3769], 'chloroacetanilide,': [496], 'thiocarbamate,': [497], 'chloro-s-triazine': [499], '(2Edwards': [503, 541], 'R.': [504, 542, 658, 669, 736, 742, 864, 877, 1005, 1018, 1056, 1363, 1376, 1402, 1466, 1609, 1615, 1752, 1905, 2025, 2038, 3517, 3851, 3993, 3999], 'Dixon': [505, 543, 739, 1612, 3996], 'D.P.': [506, 544, 646, 654, 665, 740, 810, 860, 873, 1001, 1014, 1052, 1359, 1372, 1613, 1901, 2021, 2034, 3513, 3997], 'Cobb': [507, 545], 'A.H.': [508, 546], 'Kirkwood': [509, 547], 'R.C.': [510, 548], 'Herbicides': [511, 549], 'Their': [513, 551], 'Mechanisms': [514, 552], 'Action.': [516, 554], 'Sheffield': [517, 555], 'Academic': [518, 556], 'Press,': [519, 557], 'Sheffield,': [520, 558], 'UK2000:': [521, 559], '38-91Google': [522, 560], 'GSTs': [524, 583, 682, 827, 1385, 1492, 2374], 'catalyze': [525], 'tripeptide': [533], '(γ-glutamyl-cysteinyl-glycine)': [535], 'form': [537, 4191], 'non-toxic': [538], 'S-glutathionylated': [539], 'crops,': [564], 'alternative': [565], 'GST': [566, 623, 761, 938, 1220, 1878, 1930, 2105, 2145, 3045, 3312, 3538, 3581, 3647, 3811, 4063], 'activities': [567, 2559, 2782, 3133, 3317, 3648], 'determine': [568, 2413, 2602, 2929, 3570], 'selectivity.': [570], 'For': [571, 1062, 1913, 1944], 'example,': [572], 'photobleaching': [573, 3707, 3824, 3951, 4236], 'detoxified': [581], 'legumes': [585], '(see': [586, 2223], 'Fig.': [587], '1A)': [588], 'less': [590, 854, 3963], 'efficiently': [591], '(3Frear': [594, 3770], 'D.S.': [595, 3738, 3771, 3792], 'Swanson': [596, 3731, 3772, 3785], 'H.R.': [597, 3732, 3773, 3786], 'Pestic.': [598, 1467, 3739, 3774, 3793, 3852], 'Biochem.': [599, 1510, 3740, 3775, 3794], 'Physiol.': [600, 648, 812, 1403, 3741, 3776, 3795], '1973;': [601, 3742, 3777, 3796], '3:': [602, 3743, 3778, 3797], '473-482Google': [603, 3779], 'Species-dependent': [605], 'GST-mediated': [606, 3768], 'can': [608, 4230], 'be': [609, 2646, 3803, 3863, 4182], 'explained': [610], 'differences': [612, 3289], 'expression': [615, 993, 1879, 2146, 2328, 2951, 3461, 3576, 3807, 4064], 'six': [618], 'distinct': [619], 'families': [620], 'plant': [622, 826], 'genes,': [624], 'classified': [625], 'Phi,': [628], 'Zeta,': [629], 'Tau,': [630], 'Theta,': [631], 'Lambda,': [632], 'dehydroascorbate': [634], 'reductase': [635], 'classes': [636, 794], '(4McGonigle': [637, 801], 'B.': [638, 802], 'Keeler': [639, 803], 'S.J.': [640, 804, 1806], 'Lau': [641, 805], 'S.-M.C.': [642, 806], 'Koeppe': [643, 807], 'M.K.': [644, 808], "O'Keefe": [645, 809], '2000;': [649, 813], '124:': [650, 814], '1105-1120Google': [651, 815], 'Scholar,': [652, 663, 734, 871, 1012, 1370, 2032, 3780], '5Dixon': [653], 'Lapthorn': [655, 745, 1053, 1618, 4002], 'A.': [656, 909, 1054, 1128, 1798], 'Edwards': [657, 668, 741, 863, 876, 1004, 1017, 1055, 1362, 1375, 1465, 1614, 1904, 2024, 2037, 3516, 3850, 3998], 'Genome': [659, 1057], 'Biol.': [660, 671, 730, 774, 867, 880, 1008, 1021, 1058, 1366, 1379, 1908, 2028, 2041, 3520], '2002;': [661, 673, 748, 1059, 1621, 4005], '3Google': [662, 1060], '6Dixon': [664], 'Davis': [666], 'B.G.': [667], 'J.': [670, 1758, 1810], 'Chem.': [672], '277:': [674], '30859-30869Google': [675], 'plant-specific': [678], 'primarily': [684, 3101], 'responsible': [685, 3102], 'for': [686, 788, 962, 984, 1094, 1117, 1143, 1148, 1153, 1177, 1195, 1200, 1205, 1219, 1252, 1327, 1394, 1445, 1662, 1827, 1865, 1882, 1929, 1940, 1959, 1992, 2048, 2103, 2433, 2460, 2465, 2501, 2638, 3103, 3459, 3480, 3527, 3690, 3709, 4184], 'detoxification,': [688], 'showing': [689, 1309, 1634, 1876, 2013, 2495, 2509, 3536, 3556, 3948, 3967], 'specificity': [691], 'substrate': [693, 2458, 2567, 2608, 2615, 2819], 'preference.': [694], '(GSTFs)': [697], 'highly': [699, 2232, 3864], 'active': [700, 1720, 2709, 3223, 4165], 'chloroacetanilide': [702, 3303], 'thiocarbamate': [704], 'herbicides,': [705], 'whereas': [706, 779, 3425], 'efficient': [712, 3430], 'diphenylethers': [715, 2447, 2593, 2807, 3087], 'aryloxyphenoxypropionates': [717], '(7Jepson': [718, 762], 'I.': [719, 738, 763, 1464, 1611, 3849, 3995], 'Lay': [720, 764], 'V.J.': [721, 765], 'Holt': [722, 766], 'D.C.': [723, 767], 'Bright': [724, 768], 'S.W.J.': [725, 769], 'Greenland': [726, 770], 'A.J.': [727, 746, 771, 1619, 4003], 'Mol.': [729, 773, 866, 879, 1007, 1020, 1365, 1378, 1907, 2027, 2040, 3519], '1994;': [731, 775, 910, 1129], '26:': [732, 776], '1855-1866Google': [733, 777], '8Thom': [735], 'Cummins': [737, 1610, 3994], 'Cole': [743, 861, 874, 1002, 1015, 1360, 1373, 1616, 1902, 2022, 2035, 3514, 4000], 'D.J.': [744, 862, 875, 1003, 1016, 1361, 1374, 1617, 1903, 2023, 2036, 3515, 4001], 'Biochemistry.': [747, 1620, 4004], '41:': [749, 1622, 4006], '7008-7020Google': [750, 1623, 4007], 'maize,': [753], 'GSTFs': [754], 'major': [757, 848, 2913], 'Scholar),': [778, 1408, 2045], 'soybean,': [781], 'predominate,': [783], 'difference': [786], 'accounting': [787], 'differential': [790], 'different': [793], 'two': [799, 1602, 2662, 4037], 'Scholar).We': [816], 'been': [818, 2763], 'interested': [819], 'enhancing': [821, 889], 'potential': [824], 'using': [828, 1183, 1356, 1539, 1551, 1643, 1655, 1688, 1704, 1722, 1896, 2051, 2594, 3469, 3613], 'dual': [830], 'approach': [831], 'DNA': [833, 2055, 3200], 'shuffling': [834, 2546, 2585, 3201, 3421, 3436], 'directed': [836, 3166, 3203, 3423, 4208, 4249], 'mutagenesis.': [837], '(Zea': [840], 'L.),': [842], 'expressed,': [851, 2233], 'although': [852, 3966], 'considerably': [853], 'abundant': [855], 'ZmGSTFs': [858], '(9Dixon': [859, 1000, 1358, 1900, 2020, 3512], '1998;': [868, 1009, 1367, 1811, 1909, 2029, 3521], '36:': [869, 1010, 1368, 1910, 2030, 3522], '75-87Google': [870, 1011, 1369, 1911, 2031, 3523], '10Dixon': [872, 1013, 1371, 2033], '1999;': [881, 1022, 1380, 2042], '40:': [882, 1023, 1381, 2043], '997-1008Google': [883, 1024, 1382, 2044], 'With': [885, 2247, 2716], 'intention': [887], 'we': [895, 4210], 'error-prone': [899], 'PCR': [900, 1119, 1181, 1208, 1538, 1556, 1735, 2050, 2081], '(11Stemmer': [901, 1120], 'W.P.C.': [902, 1121], 'Proc.': [903, 1122], 'Natl.': [904, 1123], 'Acad.': [905, 1124], 'Sci.': [906, 1125, 1468, 3853], 'U.': [907, 1126], 'S.': [908, 1127], '91:': [911, 1130], '10747-10751Google': [912, 1131], 'Scholar)': [913, 1472, 1624, 4008], 'randomly': [915], 'generate': [916], 'derived': [919, 2242, 2259, 2271, 3602, 4105, 4212], 'both': [921, 2449, 2513, 2672, 2943, 3086, 4154], 'ZmGSTU2.': [924, 2172, 2210, 3021, 3226], 'After': [925, 3931], 'large': [926], 'scale': [927], 'screening': [928, 2094], 'mutants': [931, 1568, 2133, 2190, 2317, 2995, 3025, 4028], 'screen,': [934], 'most': [936, 3162, 3222, 3429], 'improved': [937, 2558], 'was': [939, 1034, 1039, 1075, 1173, 1266, 1305, 1493, 1535, 1596, 1652, 1686, 1702, 1716, 1769, 2078, 2096, 2185, 2258, 2279, 2313, 2345, 2361, 2438, 2479, 2575, 2599, 2643, 2726, 2766, 2774, 2786, 2883, 2888, 2906, 2921, 2973, 3004, 3026, 3100, 3149, 3168, 3406, 3427, 3500, 3565, 3701], 'then': [940, 954, 1770, 2346, 3457, 3566, 3918], 'subjected': [941, 1536, 3073], 'selective': [943, 2791, 3706], 'further': [946, 1328, 2364, 2579], 'increase': [947, 966, 1414, 2521, 3141, 3188, 3213], 'resulting': [950, 1785, 2325, 3181, 3441], 'catalysts': [952], 'transform': [957, 1797], 'thaliana': [959, 1799], 'tested': [961], 'ability': [964], 'planta.EXPERIMENTAL': [970], 'PROCEDURESGeneration': [971], 'Mutant': [973, 1332, 2006], 'GSTs—Clones': [974], 'pGSTU1': [975, 1072, 2065], 'pGSTU2,': [977], 'pBluescript': [978, 1337, 2089], 'SK–': [979], 'vectors': [980, 1786], 'containing': [981, 1229, 1284, 1432, 1575, 1949, 2060, 2067, 2234], 'cDNA': [982, 992, 2061, 2068], 'ZmGSTU2,': [987, 1534, 1641, 2012, 2261, 3358, 3498, 3674, 3911], 'respectively,': [988, 1642], 'obtained': [990, 1651, 2485], 'library': [994], 'antibody': [995, 3511], 'screens': [996], 'detailed': [998], 'previously': [999, 1958], 'work': [1028], 'describing': [1029], 'original': [1031, 2957], 'isolation,': [1032], 'termed': [1035, 1040], 'ZmGSTV': [1036], 'ZmGSTVI,': [1041], 'new': [1043, 2868], 'designations': [1044], 'reflecting': [1045], 'unifying': [1047], 'nomenclature': [1050], '(5Dixon': [1051], '10': [1065, 1095, 1828], 'μg': [1066], 'each': [1068, 1228, 1914, 1996, 2083, 2174, 2334, 2343, 2428, 2441, 3604], 'plasmids': [1071, 1340], 'pGSTU2': [1074, 2058], 'digested': [1076, 1136, 1771, 1778], 'DNase': [1078], 'I': [1079], '(0.25–2.0': [1080], 'units)': [1081], '50': [1083, 1139, 1198, 1437], 'μlof50mm': [1084], 'Tris-HCl,': [1085], 'pH': [1086, 1282, 1430, 1484], '7.5,': [1087], '1': [1088, 1168, 2847, 2861], 'mm': [1089, 1286, 1290, 1434], 'MgCl2': [1090], 'at': [1091, 1295, 1310, 1417, 1422, 1501, 2195, 2304, 2394, 2771, 2832, 2839, 2846, 2853, 2860, 3165, 3974], '22': [1092], '°C': [1093, 1142, 1147, 1152, 1194, 1199, 1204, 1297, 1424, 1836, 1839], 'min': [1096], 'reaction': [1099, 1159, 1182], 'stopped': [1100], '2': [1102], 'μl': [1103, 1169, 1231, 1277], '0.5': [1105, 1285], 'm': [1106, 1280, 1427], 'EDTA.': [1107], 'Reactions': [1108], 'producing': [1109, 3014], 'fragments': [1110, 2056], 'mainly': [1112], '0–500': [1113], 'bp': [1114], '20': [1134, 1978, 1990, 3681, 3891], 'ng/μl': [1135], 'DNA,': [1137], 'after': [1138, 1420, 1871, 1884, 1938, 2348], 'cycles': [1140, 1191], '(94': [1141], '30': [1144, 1149, 1154, 1196, 1201, 1423], 's,': [1145, 1150, 1197, 1202], '55': [1146], '72': [1151, 1203, 3324], 's': [1155], '+': [1156], '3': [1157], 's/cycle),': [1158], '500': [1162], 'bp–20': [1163], 'kb': [1164], 'obtained,': [1166], 'mixture': [1172, 1844], 'template': [1176, 2085], 'standard': [1179], '50-μl': [1180], 'M13': [1184], 'forward': [1185], 'reverse': [1187], 'oligonucleotide': [1188], 'primers': [1189], '(30': [1190], '94': [1193], '60': [1206], 's).': [1207], 'expected': [1212], 'size': [1213, 2270], 'recloned': [1215], 'into': [1216, 1561, 1718, 1732, 1742, 1776, 1789, 2088, 2321], 'pBluescript.Screening': [1217], 'Mutants': [1218, 1308, 2617, 3374], 'Activity—Wells': [1221], 'sterile': [1223], 'flat-bottomed,': [1224], '96-well': [1225], 'microtiter': [1226, 2092], 'plates,': [1227], '200': [1230, 1276], 'LB': [1233], 'medium': [1234, 1265], 'supplemented': [1235], 'antibiotics,': [1237], 'inoculated': [1239], 'individual': [1241, 1888], 'bacterial': [1243], 'colonies': [1244, 2102], 'taken': [1245], 'master': [1248], 'plate': [1249, 2093], 'grown': [1251, 1818, 1826, 1954], '16': [1253], 'h': [1254], 'stationary': [1256], 'phase.': [1257], 'plates': [1259], 'centrifuged,': [1261], 'growth': [1264, 3452], 'removed': [1267], 'aspiration': [1269], 'prior': [1270, 1852], 'resuspending': [1272], 'bacteria': [1274, 2205, 3038, 3059], '0.1': [1279, 1426], 'glycine-NaOH,': [1281], '9.5,': [1283, 1431], '5': [1289, 1433, 2833, 2840, 2854], 'glutathione.': [1291], 'Plates': [1292], 'incubated': [1294], '37': [1296], '(2–4': [1298], 'h),': [1299], 'release': [1302, 2114], 'p-nitrophenol': [1304, 1319, 2117], 'visually': [1306, 3929], 'monitored.': [1307], 'least': [1311, 2196], '5-fold': [1313, 2197, 3140], 'faster': [1314], 'accumulation': [1315], 'yellow': [1318], 'ZmGSTU': [1323], 'clones': [1324, 2154, 2214], 'selected': [1326, 1881, 1957, 3407], 'characterization.Analysis': [1329], 'Recombinant': [1331], 'GSTs—GSTUs': [1333], 'cloned': [1334, 2087], 'either': [1336, 2207], 'pET': [1339], 'Escherichia': [1342], 'coli': [1343, 3030], 'cultured': [1345], 'without': [1346], 'isopropyl-1-thio-β-d-galactopyranoside': [1347], 'induction,': [1348], 'respective': [1351, 1738], 'recombinant': [1352, 1491, 2373, 2803, 3050], 'proteins': [1353, 2219], 'purified': [1355, 1490, 2799, 3340], 'S-hexylglutathione-Sepharose': [1357], 'Purified': [1384], 'extensively': [1387], 'dialyzed': [1388], 'remove': [1390], 'S-hexylglutathione': [1391], 'assayed': [1393, 1928, 2376, 3310, 3342, 3526], 'CDNB': [1397, 2627, 2673, 3143, 3156, 3261], '(1-chloro-2,4-dinitrobenzene)': [1398], '(12Edwards': [1401], 'Plant.': [1404], '1996;': [1405], '98:': [1406], '594-604Google': [1407], 'latter': [1410, 3147, 3555], 'following': [1412], 'absorbance': [1416, 1500], '400': [1418], 'nm': [1419, 1503], 'incubation': [1421], 'glycine-NaOH': [1428, 1482], 'buffer,': [1429, 1483], 'μm': [1438, 1979, 3682, 3892], 'High': [1440], 'pressure': [1441], 'liquid': [1442], 'chromatography-based': [1443], 'assays': [1444, 1475, 3546], 'described': [1454], '(13Andrews': [1455, 3840], 'C.J.': [1456, 3841], 'Skipsey': [1457, 3842], 'M.': [1458, 3843], 'Townson': [1459, 3844], 'J.K.': [1460, 3845], 'Morris': [1461, 3846], 'C.': [1462, 1754, 1756, 3847], 'Jepson': [1463, 3848], '1997;': [1469, 3854], '51:': [1470, 3855], '213-222Google': [1471, 3856], 'except': [1473, 3495], 'fomesafen': [1477, 2384, 2487, 3135], 'carried': [1479], 'out': [1480], '9.5.': [1485], 'protein': [1487, 1595, 1942, 2916, 3351, 3508], 'concentration': [1488], 'measured': [1494], 'based': [1495, 2111], 'on': [1496, 1598, 2112, 2586, 2607, 2746, 2789, 2915, 2932, 3375], 'calculated': [1498, 2439, 2831, 2838, 2845, 2852, 2859], 'UV': [1499], '280': [1502], '(14Gill': [1504], 'S.C.': [1505], 'von': [1506], 'Hippel': [1507], 'P.H.': [1508], 'Anal.': [1509], '1989;': [1511], '182:': [1512], '319-326Google': [1513], 'Scholar).Site-directed': [1514], 'Mutagenesis—The': [1515], 'Q115L': [1516], 'EFD6': [1520, 2515, 2666, 2689, 2725, 2873, 2882, 3096, 3177, 3637, 3641], '(enhanced': [1521], '6),': [1525], 'corresponding': [1526, 2296], '113th': [1529], 'amino': [1530], 'acid': [1531, 2982], 'specific': [1540, 2177, 3316, 3559], 'mutagenic': [1541], 'primers,': [1542], 'primer': [1546], 'T7': [1549], 'promoter,': [1550], 'pEFD6': [1552, 1562], 'template.': [1555], 'subcloned': [1559, 1731, 2320], 'back': [1560, 3007], 'give': [1564, 3818, 4231], 'constructs': [1565], 'expressing': [1566, 2206, 2216, 3039, 3060, 3497, 3909, 3959], 'EFD6–115E,': [1569, 2997], 'EFD6–115N,': [1570, 2996], 'EFD6–115F,': [1571, 2998, 3061], 'EFD6–115A,': [1572, 3000, 3062, 3553], 'EFD6–115Q': [1574, 3064, 3079], 'mutations': [1576], 'leucine': [1578, 2303], '115': [1579, 2306, 2773, 3003, 3099, 3118, 3175, 3208, 4132], 'glutamic': [1581, 2981], 'acid,': [1582], 'asparagine,': [1583], 'phenylalanine,': [1584], 'alanine,': [1585], 'glutamine,': [1587, 2960], 'respectively.Modeling': [1588], 'Fluorodifen': [1590, 3378], 'Binding': [1591], 'ZmGSTU2—The': [1593], 'modeled': [1597], 'templates': [1600], 'wheat': [1606, 3991], 'TaGSTU4': [1607], '(8Thom': [1608, 3992], 'rice': [1626, 3718, 4010], 'OsGSTU1': [1627, 1693], '(Protein': [1628, 4011], 'Data': [1629, 4012], 'Bank': [1630, 4013], 'accession': [1631, 4014], 'code': [1632], '1OYJ),': [1633], '39': [1635], '63%': [1637], 'sequence': [1638, 2015, 2169, 2257, 2659, 2740, 2761], 'identity': [1639, 2016], 'web-based': [1645], 'utility': [1646], 'SWISSMODEL': [1647], '(www.expasy.ch/swissmod/SWISS-MODEL.html).': [1648], 'model': [1650, 1699, 2614, 2817, 4087, 4170], 'manually': [1653], 'analyzed': [1654, 2363, 3458, 3607], 'graphics': [1657], 'program': [1658], 'QUANTA': [1659], '(Accelyrs': [1660, 1708], 'Inc.)': [1661], 'steric': [1663, 1680], 'clashes': [1664], 'chemical': [1666], 'sensibility.': [1667], 'side': [1672, 2875], 'chain': [1673], 'Gln-115': [1676], 'resulted': [1677], 'clash': [1681], 'Tyr-170': [1683], 'therefore': [1685, 2787, 2974, 3802], 'remodeled': [1687], 'water': [1689], 'positions': [1690], 'guide.': [1696], 'molecular': [1698, 3619, 4019], 'INSIGHTII': [1705], 'CATALYST': [1707], 'Inc.),': [1709], 'suitable': [1712], 'low': [1713, 2396], 'energy': [1714], 'docked': [1717, 4145], 'site': [1721, 2710, 4166], 'QUANTA.Generation': [1723], 'Analysis': [1725, 2150], 'Transgenic': [1727], 'Plants—GST': [1728], 'pRT108': [1733], 'pET-vectors': [1739], 'ligated': [1741, 1775], 'NcoI': [1744], 'BamHI': [1746], 'sites': [1747], 'vector': [1750, 3627, 3671, 3936], '(15Töpfer': [1751], 'Maas': [1753], 'Höricke-Grandpierre': [1755], 'Schell': [1757], 'Steinbiss': [1759], 'H.-H.': [1760], 'Methods': [1761], 'Enzymol.': [1762], '1993;': [1763], '217:': [1764], '66-78Google': [1765], 'This': [1767, 2407, 2478, 2642, 2971, 3402], 'cassette': [1768], 'HindIII': [1773], 'similarly': [1777], 'pCAMBIA': [1779], '3300': [1780], '(CAMBIA,': [1781], 'Canberra,': [1782], 'Australia).': [1783], 'transformed': [1788, 3549, 3669], 'Agrobacterium': [1790], 'tumefaciens': [1791], 'strain': [1792], 'C58C3': [1793], 'ecotype': [1800], 'Columbia': [1801], 'floral': [1803], 'dipping': [1804], '(16Clough': [1805], 'Bent': [1807], 'A.F.': [1808], '16:': [1812], '735-743Google': [1813], 'Dipped': [1815], 'plants': [1816, 1824, 1868, 3382, 3455, 3601, 3668, 3859, 3889, 3908, 3927, 3958], 'seed': [1820], 'set,': [1821], 'T1': [1823, 1874, 3442], 'days': [1829, 1923, 1994, 3692], 'greenhouse': [1832], '(16-h': [1833], 'photoperiod;': [1834], '23': [1835], 'day;': [1837], '18': [1838], 'night)': [1840], '4:1': [1843], 'general': [1846], 'purpose': [1847], 'compost': [1848], 'silver': [1850], 'sand': [1851], 'spraying': [1854, 1966, 3885], '0.02%': [1856], '(w/v)': [1857], 'glufosinate': [1858, 1968], 'ammonium': [1859, 1969], '(1': [1860], 'ml/35': [1861], 'cm2)': [1862], 'select': [1864], 'transformants.': [1866], 'Surviving': [1867], 'resprayed': [1870], '7': [1872], 'days.': [1873], 'lines': [1875, 3494, 3535], 'good': [1877, 3066], 'propagation': [1883], 'analyzing': [1885], 'extracts': [1886, 3655], 'rosette': [1889], 'leaves': [1890], 'SDS-PAGE': [1892, 3609], 'Western': [1894, 1936, 3467, 3611], 'blotting': [1895, 1937, 3468, 3612], 'anti-ZmGSTU1–2': [1898, 3615], 'serum': [1899], 'line': [1915], 'interest,': [1917], '∼10': [1918], 'whole': [1919], 'T2': [1920, 1952, 3444, 3454, 3592, 3904], 'seedlings': [1921, 1953], '(14': [1922], 'old)': [1924], 'extracted': [1926], 'immunoreactive': [1933], 'polypeptides': [1934, 3051, 3490], 'normalizing': [1939], 'total': [1941, 3662], 'content.': [1943], 'spray': [1946], 'trials,': [1947], 'cells': [1948], 'five': [1950], '25-day-old': [1951, 3887], 'compost,': [1956], 'presence': [1961], 'T-DNA': [1963], 'insertions': [1964], 'kill': [1971], 'segregating': [1972], 'wild-type': [1973, 2802], 'plants,': [1974, 3941], 'sprayed': [1976, 3869], 'formulated': [1981], '1%': [1983], '(v/v)': [1984, 1988], 'acetone': [1985], '0.01%': [1987], 'Tween': [1989], 'daily': [1991, 3679], '14': [1993, 3691], '23-cm2': [1997], 'cell': [1998], 'receiving': [1999], '1-ml': [2001], 'application.RESULTSGeneration': [2002], 'Characterization': [2004], 'Reconstructive': [2009], 'PCR—ZmGSTU1': [2010], '72%': [2014], 'one': [2018], 'another': [2019], 'employed': [2047, 2922], 'equal': [2052], 'amounts': [2053], '(pBluescript': [2059, 2066], 'encoding': [2062, 2069], 'ZmGSTU2)': [2063, 2312], 'ZmGSTU1).': [2070], 'Following': [2071], 'recombination,': [2074], 'product': [2077], 'amplified': [2079], 'reassembled': [2084], 'SK–.': [2090], 'procedure': [2095], 'assay': [2099], 'colored': [2116, 4091, 4109, 4115, 4155, 4193], '(Fig.': [2118, 2245, 2274, 2387, 2743, 3484, 3531, 3954, 4049], '1,': [2119, 3623], 'B).': [2122], 'As': [2123, 3109, 3227, 3595, 3664], 'ZmGSTUs,': [2128, 3267, 3390], 'significant': [2130], 'proportion': [2131], 'showed': [2134, 2155, 2455, 3286, 3315, 3446, 3881], 'moderately': [2135], 'activity,': [2137], 'largely': [2138, 3901], 'relatively': [2141, 2395], 'levels': [2143, 4061], 'bacteria.': [2149], 'three': [2152], 'they': [2157], 'chimeras': [2159, 2654], 'N-terminal': [2162, 2733, 4043, 4055], 'portion': [2163, 2734], 'remaining': [2168, 2739], 'composed': [2170], 'case,': [2175], 'pure': [2181, 3322, 3350, 3362], 'enzyme': [2182, 2199, 2434, 2587, 2887, 2933, 3126, 3225, 3254, 3285, 3414, 3433, 4101, 4229], 'little': [2186], 'affected.': [2187], 'However,': [2188, 2597], '(as': [2201], 'judged': [2202], 'visually)': [2203], 'These': [2211, 2541, 3330, 4051], 'pEFD1–7,': [2215], 'EFD1–7,': [2220], 'sequenced': [2222], 'supplementary': [2224], 'information).': [2225], 'Six': [2226], 'EFDs': [2230, 2454, 2472, 2756, 2902, 3405], 'N': [2235], 'termini': [2236], '(between': [2237], '48': [2238], '62': [2240], 'residues)': [2241], '1C).': [2246, 2275, 2744], 'exception': [2249, 2357, 2718], 'EFD6,': [2251, 2277, 3230, 3391], 'remainder': [2253], 'coding': [2256], 'interrupted': [2262], 'region,': [2265], 'regions,': [2267], 'varying': [2269], 'there': [2278], 'no': [2280], 'insertion': [2282], 'C-terminal': [2285, 4046], 'half': [2286], 'protein,': [2289, 3323, 3363], 'instead': [2291], 'missense': [2294], 'substitution': [2299, 2721, 2770, 2880, 2910, 3172, 3233], 'glutamine': [2301, 3009], '(equivalent': [2307], '113': [2310], 'identified.The': [2314], 'individually': [2319, 3384], 'pET-11d': [2323], 'plasmid,': [2324], '28-kDa': [2331, 3489], 'polypeptide': [2332], 'case': [2335, 2442], 'determined': [2337, 2637, 3110, 3337, 3651], 'SDS-PAGE.': [2339], 'kinetics': [2341], 'examined': [2347], 'purifying': [2349], 'affinity': [2353], 'chromatography,': [2354], 'EFD7,': [2359], 'instability': [2368], 'its': [2370, 3753], 'related': [2381, 2453, 2566], 'acifluorfen': [2386, 2525, 2810, 3293], '1A).': [2388], 'All': [2389], 'substrates': [2391], 'concentrations': [2397], '(50': [2398], 'μm)': [2399], 'limited': [2403, 2572], 'solubility': [2404, 2425], 'water.': [2406], 'made': [2408], 'it': [2409, 2574, 2598, 2765], 'impossible': [2410], 'directly': [2412], 'Km': [2415, 2622, 2669, 3154, 3248, 4068], 'values': [2416, 2464, 2500, 2623, 2631, 2670, 3331, 3660], 'substrates,': [2419, 3313], 'above': [2423], 'limit': [2426], 'case.': [2429], 'Instead,': [2430], 'value': [2432, 3239], '(kcat/Km)': [2437], '(Table': [2443, 3077, 4078], 'I).': [2444, 3078], 'Of': [2445], 'tested,': [2448], 'marked': [2457], 'preference': [2459], 'kcat/Km': [2463, 2502, 2523, 3113, 3190, 3238, 3291], 'all': [2470, 3492], 'enzymes.': [2477], 'contrast': [2481], 'results': [2484, 2542, 2696], 'acifluorfen,': [2489, 3137], 'EFD1–5': [2494, 2618, 4032], 'similar': [2496, 3082, 3128, 3257, 3334, 3977], 'slightly': [2498], 'reduced': [2499, 3132], 'ZmGSTU1,': [2505, 3673, 3910], 'only': [2507, 3287, 3534], 'appreciable': [2510], 'herbicides.': [2514], 'did': [2516, 3273], 'show': [2517], 'modest': [2519], '4-fold': [2520], 'greater': [2533, 3237, 3558, 3754, 3820], '18-fold': [2535], 'enhancement': [2536], 'substrate.': [2540, 3145], 'suggested': [2543, 2698], 'gene': [2545, 2584, 3420], 'studies': [2547], 'set': [2551], 'selectively': [2557], 'closely': [2565], 'chemistries.': [2568], 'Constrained': [2569], 'solubilities,': [2573], 'possible': [2577, 2600], 'investigate': [2580], 'effects': [2582], 'binding': [2588, 2609, 4029, 4186], 'turnover': [2590, 2611], 'kinetic': [2595, 2750, 3075, 3258], 'analysis.': [2596], 'effect': [2604, 2876, 2914, 2931], 'CDNB.': [2616, 2863], 'inclusive': [2619], 'apparent': [2621], 'GSH': [2625], 'associated': [2629, 3751], 'Vmax': [2630], 'within': [2634], 'range': [2636], 'GSTUs.': [2641], 'what': [2644], 'would': [2645, 3801, 3817], 'predicted': [2647, 4180], 'effectively': [2653, 3013], 'modular': [2656], 'blocks': [2657, 2759], 'parents.': [2663], 'contrast,': [2665, 3058], 'increased': [2668, 4219], 'GSH,': [2675], 'coupled': [2676], 'decreased': [2679], 'Vmax.': [2680, 3160], 'Taken': [2681], 'singular': [2685], 'changes': [2686, 4052, 4095], 'seen': [2687, 3169], 'cleavage': [2692], 'collectively': [2697], 'undergone': [2703], 'more': [2705, 2949, 3723], 'radical': [2706], 'topography': [2711], 'EFDs.': [2715], '115,': [2724], 'otherwise': [2727], 'simple': [2729], 'chimera': [2730, 3017], 'Based': [2745], 'essentially': [2748, 3256, 4034], 'conserved': [2749], 'characteristics': [2751, 2797, 3081, 3259], 'chimeric': [2755, 2901], 'shuffled,': [2764], 'concluded': [2767], 'key': [2776, 3206], 'factor': [2777], 'novel': [2780], 'EFD6.': [2784, 3195, 3280], 'Attention': [2785], 'focused': [2788], 'residue.Table': [2795], 'IKinetic': [2796], 'mutated': [2800, 2926, 2975], '(Flu),': [2809], '(Aci),': [2811], 'Fomesafen': [2813], '(Fom)': [2814], 'spectrophotometric': [2818], 'GSHEnzymekcat/Km': [2824], '(Flu)kcat/Km': [2825], '(Aci)kcat/Km': [2826], '(Fom)kcat/Km': [2827], '(CDNB)Km': [2828], '(CDNB)aApparent': [2829, 2836], 'values,': [2830, 2837, 2844, 2851, 2858, 3114], 'mM': [2834, 2841, 2848, 2855, 2862], 'glutathione.Vmax': [2835], 'glutathione.Km': [2842], '(GSH)bApparent': [2843], 'CDNB.M-1·S-1M-1·S-1M-1·S-1M-1·S-1μMnanokatal/mgmMZmGSTU12301.636.7018,400101011100.56ZmGSTU2400ND0.42300,00011519101.72EFD152900.770.98219,00020622901.95EFD237001.101.26142,0001369300.76EFD375500.960.84343,00019719501.15EFD423600.621.50185,00020417301.18EFD525000.681.70144,00021215001.05EFD642907.609.10920018007604.75EFD6-115Q220ND0.52193,0001068800.92EFD6-115F50404.203.8047,4005348700.74EFD6-115A11,7004.902.60150,00020010600.78a': [2849], 'Apparent': [2850, 2857], 'glutathione.b': [2856], 'Open': [2864], 'table': [2865], 'tab': [2869], 'Directed': [2870], 'Mutagenesis': [2871], '—A': [2874], 'L115Q': [2879], 'predominantly': [2889], 'insoluble': [2893], 'fraction': [2894], 'E.': [2896, 3029], 'coli,': [2897], 'unlike': [2898], 'analyzed.': [2903], 'Again,': [2904], 'consistent': [2907], 'having': [2911], 'structure.': [2917], 'Rational': [2918], 'site-directed': [2919], 'substitute': [2924], 'aim': [2937], 'finding': [2939], 'high': [2944, 4060], 'stable': [2950], 'soluble': [2954, 3069], 'protein.': [2955, 3035], 'replaced': [2959], 'mediumsized': [2962], 'neutral': [2963], 'residue,': [2964], 'leucine,': [2966], 'smaller': [2968], 'hydrophobic': [2969], 'residue.': [2970], 'asparagine': [2977], '(neutral': [2978], 'smaller),': [2980], '(acidic,': [2983], 'same': [2984], 'size),': [2985], 'phenylalanine': [2986, 3120], '(hydrophobic,': [2987, 2991], 'large),': [2988], 'alanine': [2990, 3232], 'small),': [2992], 'respectively.': [3001, 3329], 'Residue': [3002], 'also': [3005, 3234], 'mutagenized': [3006], 'yield': [3011], 'EFD6–115Q,': [3012], 'unmutated': [3016], 'Each': [3022], 'LacZ': [3033], 'fusion': [3034], 'Lysates': [3036], 'EFD6–115E': [3040], 'EFD6–115N': [3042], 'negligible': [3044], 'misfolded': [3049], 'precipitated': [3052], 'inclusion': [3055], 'bodies.': [3056], 'gave': [3065, 3124, 3894], 'yields': [3067], 'GSTs,': [3070], 'analysis': [3076], 'confirming': [3090], 'affecting': [3097], 'cleaving': [3107, 3482], 'comparing': [3112], 'produce': [3122], 'EFD6–115F': [3123], 'effected': [3150], 'decreasing': [3152], 'rather': [3157, 3719], 'increasing': [3159], 'successful': [3163], 'attempt': [3164], 'alanine.': [3179], 'EFD6–115A': [3183, 3314, 3394, 3409, 3676, 3916, 3960], 'almost': [3185], '3-fold': [3187], 'Overall,': [3196], 'combination': [3198, 3403, 3418, 4204], 'caused': [3210], 'restored': [3235], 'again': [3242], 'result': [3245], 'lowering': [3247], 'substrate,': [3251], 'suggesting': [3269], 'structural': [3277, 4086], 'problems': [3278], 'Similarly,': [3281], '"optimized"': [3283], 'minor': [3288], 'fomesafen,': [3295], 'resembling': [3297], 'structurally': [3301], 'unrelated': [3302], 'acetochlor,': [3305], 'alachlor,': [3306], 'metolachlor': [3308], '112': [3319], 'picokatal/mg': [3320, 3348, 3360], 'picokatal/mg,': [3325, 3328, 3353, 3356, 3365, 3368], '144': [3327], 'very': [3333], 'those': [3336, 3496, 3548], 'ZmGSTUs': [3341], 'under': [3343, 3395, 3450], 'identical': [3344], 'conditions': [3345], '(ZmGSTU1,': [3346], '160': [3347], '68': [3352], '192': [3355], 'respectively;': [3357], '215': [3359], '91': [3364], '157': [3367], 'respectively).Effect': [3369], 'Expression': [3371], 'Tolerance': [3376], 'Planta—Transgenic': [3380], 'engineered': [3385], 'express': [3387], 'EFD3,': [3392], 'control': [3397, 3628], 'CaMV35S': [3400], 'promoter.': [3401], 'represented': [3410, 4149, 4173], 'through': [3416, 3767], 'mutagenesis,': [3424, 4209], 'EFD3': [3426, 3551], 'alone.': [3437], 'None': [3438], 'transgenics': [3445], 'any': [3447], 'abnormal': [3448], 'phenotype': [3449], 'normal': [3451], 'conditions.': [3453], 'introduced': [3464], 'antiserum': [3471, 3487], 'raised': [3472], 'ZmGSTU1–2': [3475], 'heterodimer': [3476], 'assaying': [3479], '2A).': [3485], 'recognized': [3488], 'GSTU-transformed': [3493], 'weak': [3504], 'immunoreactivity': [3505], '2A),': [3532], 'controls.': [3563], 'It': [3564, 3800], 'interest': [3568], 'whether': [3571], 'transgenic': [3575, 3593], 'could': [3583], 'confer': [3584], 'planta.Fig.': [3589], '2Analysis': [3590], 'Arabidopsis.': [3594], 'shown': [3596, 3657, 3665, 4139, 4188], 'A,': [3598], 'pools': [3599], 'construct': [3605], 'serum.': [3616], 'Lane': [3617], 'M,': [3618], 'mass': [3620], 'markers': [3621], '(kDa);': [3622], 'wild': [3624], 'type;': [3625], '2,': [3626], '(pCAMBIA3300);': [3629], '3,': [3630], 'ZmGSTU1;': [3631], '4,': [3632], 'ZmGSTU2;': [3633], '5,': [3634], 'EFD3;': [3635], '6,': [3636], '(line': [3638, 3642], '1);': [3639], '7,': [3640], '2);': [3643], '8,': [3644], 'EFD6–115A.': [3645], 'duplicate': [3653], 'crude': [3654], 'mean': [3659], '(picokatal/mg': [3661], 'protein).': [3663], 'B,': [3667, 4160], 'alone,': [3672], 'treated': [3678, 3919, 3957], '(sprayed)': [3684], 'left': [3687], 'untreated': [3688], '(unsprayed)': [3689], 'glasshouse.View': [3695], 'Large': [3696, 4198], 'Image': [3697, 4199], 'Figure': [3698, 4200], 'ViewerDownload': [3699, 4201], '(PPT)Fluorodifen': [3700], 'originally': [3702], 'developed': [3703], 'use': [3710], 'peanuts,': [3714], 'soybean': [3715], 'cotton,': [3716], 'sensitive': [3724, 3865], 'Brassica': [3725], 'species': [3726, 3816], '(17Shimabukuro': [3727], 'R.H.': [3728, 3782], 'Lamoureux': [3729, 3783], 'G.L.': [3730, 3784], 'Walsh': [3733, 3787], 'W.C.': [3734, 3788], 'Stafford': [3735, 3789], 'L.E.': [3736, 3790], 'Frear': [3737, 3791], '483-494Google': [3744, 3798], 'tolerant': [3759, 3838], 'susceptible': [3764, 3815], 'principally': [3766], '17Shimabukuro': [3781], 'anticipated': [3804], 'normally': [3814], 'visibly': [3819], 'characteristic': [3831], 'feature': [3832], 'even': [3836], 'found': [3861], 'when': [3868, 4242], 'unformulated': [3872], 'leaf': [3873], 'drench': [3874], 'treatment.': [3875], 'Careful': [3876], 'adjustment': [3877], 'treatments': [3880], 'repeated': [3884], 'sublethal': [3896], 'severe': [3898], 'photobleaching,': [3899, 3969], 'arrested': [3902], 'growth.': [3903], 'generation': [3905], 'controls': [3906], 'dosing': [3923], 'regime,': [3924], 'assessed.': [3930], '14-day': [3933], 'treatment,': [3934], 'control,': [3937], 'ZmGSTU2-expressing': [3940], 'severely': [3943], 'stunted': [3944], 'foliage': [3947], 'signs': [3949], 'necrosis': [3953], '2B).': [3955], 'Similarly': [3956], 'much': [3962], 'damaged,': [3964], 'some': [3968], 'continued': [3971], 'grow': [3973], 'unsprayed': [3981], 'plants.The': [3982], 'recent': [3983], 'availability': [3984], 'crystal': [3986], 'structures': [3987], 'number': [4015], '1OYJ)': [4016], 'has': [4017], 'permitted': [4018], 'regions': [4038, 4119], 'domain': [4044], 'domain,': [4047], 'respectively': [4048], '3A).': [4050], 'region': [4056, 4104], 'correlated': [4058], 'lower': [4067], 'I).Fig.': [4079], '3A,': [4080], 'ribbon': [4082], 'representation': [4083], 'monomer': [4090], 'highlight': [4093], 'representative': [4098], '(EFD2).': [4102], 'green,': [4110], 'light': [4116], 'blue': [4117], 'differ': [4121], 'highlighted': [4124, 4134], 'dark': [4126], 'blue,': [4127], 'critical': [4130], 'red.': [4136], 'ball-and-stick': [4141], 'representation,': [4142], 'stick': [4151, 4190], 'form,': [4152], 'according': [4156, 4194], 'atom': [4158, 4196], 'type.': [4159], 'detail': [4162], 'Van': [4175], 'der': [4176], 'Waals': [4177], 'spheres.': [4178], 'Residues': [4179], 'important': [4183], 'type.View': [4197], '(PPT)DISCUSSIONThrough': [4202], 'random': [4206], 'EFD-115A,': [4215], 'greatly': [4218], 'capacity': [4220], 'demonstrated': [4225], 'protection': [4233], 'against': [4234], 'injury': [4237], 'incurred': [4238], 'planta.': [4245], 'Although': [4246], 'forced': [4247], 'evolution': [4248], 'tow': [4250]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2139860372', 'counts_by_year': [{'year': 2024, 'cited_by_count': 2}, {'year': 2023, 'cited_by_count': 4}, {'year': 2022, 'cited_by_count': 6}, {'year': 2021, 'cited_by_count': 4}, {'year': 2020, 'cited_by_count': 2}, {'year': 2019, 'cited_by_count': 7}, {'year': 2018, 'cited_by_count': 4}, {'year': 2017, 'cited_by_count': 8}, {'year': 2016, 'cited_by_count': 5}, {'year': 2015, 'cited_by_count': 5}, {'year': 2014, 'cited_by_count': 5}, {'year': 2013, 'cited_by_count': 7}, {'year': 2012, 'cited_by_count': 4}], 'updated_date': '2024-12-09T01:49:47.803841', 'created_date': '2016-06-24'}