Title: Glutathione S-Transferase Activities of S-Type and L-Type Thioltransferases from Arabidopsis thaliana
Abstract:The glutathione S-transferase (GST) activities of S-type and L-type thioltransferases (TTases), which are purified from the seeds and leaves of Arabidopsis thaliana, respectively, were identified and ...The glutathione S-transferase (GST) activities of S-type and L-type thioltransferases (TTases), which are purified from the seeds and leaves of Arabidopsis thaliana, respectively, were identified and compared. The S-type and L-type TTases showed values of 9.72 mM and 3.18mM on 1-chloro-2,4-dinitrobenzene (CDNB), respectively, indicating the L-type TTase has higher affinity for CDNB. The GST activity of the L-type TTase was rapidly inactivated after being heated at or higher. The GST activity of the S-type TTase remains active in a range of . inhibited the GST activity of the S-type TTase, whereas and inhibited the GST activity of the L-type TTase. Our results suggest that the GST activities of two TTases of Arabidopsis thaliana may have different catalytic mechanisms. The importance of the co-existence of TTAse and GST activities in one protein remains to be elucidated.Read More
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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Cited By Count: 10
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