Publication Information

{'id': 'https://openalex.org/W2349738535', 'doi': None, 'title': 'A Modified Method of QuikChange Site-Directed Mutagenesis', 'display_name': 'A Modified Method of QuikChange Site-Directed Mutagenesis', 'publication_year': 2008, 'publication_date': '2008-01-01', 'ids': {'openalex': 'https://openalex.org/W2349738535', 'mag': '2349738535'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'http://en.cnki.com.cn/Article_en/CJFDTOTAL-XDZK2008S2064.htm', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764748148', 'display_name': 'Journal of Xiamen University', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/P4323238804', 'host_organization_name': 'Ministry of Education of China', 'host_organization_lineage': ['https://openalex.org/P4323238804'], 'host_organization_lineage_names': ['Ministry of Education of China'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': [], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5100403643', 'display_name': 'Rui Chen', 'orcid': 'https://orcid.org/0000-0002-4387-9735'}, 'institutions': [], 'countries': [], 'is_corresponding': True, 'raw_author_name': 'Chen Rui', 'raw_affiliation_strings': ['-Chuan,LIU Run-Zhong*'], 'affiliations': [{'raw_affiliation_string': '-Chuan,LIU Run-Zhong*', 'institution_ids': []}]}], 'institution_assertions': [], 'countries_distinct_count': 0, 'institutions_distinct_count': 0, 'corresponding_author_ids': ['https://openalex.org/A5100403643'], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.0, 'has_fulltext': False, 'cited_by_count': 2, 'citation_normalized_percentile': {'value': 0.464553, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 70, 'max': 74}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T12029', 'display_name': 'DNA and Biological Computing', 'score': 0.8326, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T12029', 'display_name': 'DNA and Biological Computing', 'score': 0.8326, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12828', 'display_name': 'Plant Genetic and Mutation Studies', 'score': 0.8301, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10434', 'display_name': 'Chromosomal and Genetic Variations', 'score': 0.8242, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/site-directed-mutagenesis', 'display_name': 'Site-directed mutagenesis', 'score': 0.8560801}, {'id': 'https://openalex.org/keywords/directed-mutagenesis', 'display_name': 'Directed mutagenesis', 'score': 0.57728404}], 'concepts': [{'id': 'https://openalex.org/C103408237', 'wikidata': 'https://www.wikidata.org/wiki/Q2642668', 'display_name': 'Site-directed mutagenesis', 'level': 4, 'score': 0.8560801}, {'id': 'https://openalex.org/C16318435', 'wikidata': 'https://www.wikidata.org/wiki/Q149299', 'display_name': 'Mutagenesis', 'level': 4, 'score': 0.8550714}, {'id': 'https://openalex.org/C69034178', 'wikidata': 'https://www.wikidata.org/wiki/Q4680754', 'display_name': 'Directed mutagenesis', 'level': 4, 'score': 0.57728404}, {'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.5250504}, {'id': 'https://openalex.org/C143065580', 'wikidata': 'https://www.wikidata.org/wiki/Q3285695', 'display_name': 'Mutant', 'level': 3, 'score': 0.5027008}, {'id': 'https://openalex.org/C70721500', 'wikidata': 'https://www.wikidata.org/wiki/Q177005', 'display_name': 'Computational biology', 'level': 1, 'score': 0.4616576}, {'id': 'https://openalex.org/C9418097', 'wikidata': 'https://www.wikidata.org/wiki/Q440681', 'display_name': 'Directed evolution', 'level': 4, 'score': 0.4173698}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.39619273}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.3714403}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.12568578}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': False, 'landing_page_url': 'http://en.cnki.com.cn/Article_en/CJFDTOTAL-XDZK2008S2064.htm', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764748148', 'display_name': 'Journal of Xiamen University', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/P4323238804', 'host_organization_name': 'Ministry of Education of China', 'host_organization_lineage': ['https://openalex.org/P4323238804'], 'host_organization_lineage_names': ['Ministry of Education of China'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 0, 'referenced_works': [], 'related_works': ['https://openalex.org/W3120353831', 'https://openalex.org/W3044120116', 'https://openalex.org/W3025194208', 'https://openalex.org/W2528862892', 'https://openalex.org/W2527791904', 'https://openalex.org/W2394273545', 'https://openalex.org/W2389922813', 'https://openalex.org/W2374630094', 'https://openalex.org/W2354279342', 'https://openalex.org/W2143736123', 'https://openalex.org/W2070864278', 'https://openalex.org/W2070204219', 'https://openalex.org/W2060918019', 'https://openalex.org/W2057405809', 'https://openalex.org/W2037411762', 'https://openalex.org/W1995263127', 'https://openalex.org/W1994262982', 'https://openalex.org/W1981950626', 'https://openalex.org/W1973868765', 'https://openalex.org/W1599727139'], 'abstract_inverted_index': {'A': [0], 'traditional': [1, 41], 'method': [2, 69], 'that': [3, 52, 63], 'introduced': [4], 'mutations': [5], 'to': [6, 85, 89], 'DNA': [7, 54, 82], 'was': [8, 29, 36], 'the': [9, 40, 47, 53, 64], 'QuikChangeTM': [10, 18], 'Site-Directed': [11, 13, 19, 43], 'Mutagenesis,calledOne-Step': [12], 'Mutagenesis.However,it': [14], 'had': [15], 'low': [16, 91], 'efficiency.The': [17], 'Mutagenesis': [44], 'Kit': [21], 'could': [22, 55, 70], 'create': [23], 'mutants': [24], 'about': [25], '80%': [26], 'efficiency,but': [27], 'it': [28, 93], 'too': [30], 'expensive.The': [31], 'protocol': [32, 73], 'in': [33], 'this': [34, 67], 'paper': [35], 'a': [37, 78], 'modification': [38], 'of': [39, 49, 66, 81], 'One-Step': [42], 'and': [45], 'simplified': [46], 'design': [48], 'primers': [50], 'so': [51], 'be': [56, 75, 86], 'easily': [57], 'mutated': [58, 87], 'by': [59], 'novice.Our': [60], 'results': [61], 'indicated': [62], 'efficiency': [65], 'modified': [68], 'reach': [71], '96.22%.This': [72], 'maight': [74], 'applied': [76], 'for': [77], 'large': [79], 'number': [80], 'samples': [83], 'needed': [84], 'due': [88], 'its': [90], 'cost.So': [92], 'is': [94], 'worth': [95], 'widely': [96], 'application.': [97]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2349738535', 'counts_by_year': [{'year': 2014, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 1}], 'updated_date': '2024-12-12T07:05:52.512886', 'created_date': '2016-06-24'}