Title: The measurement of urinary tetrahydroaldosterone by radioimmunoassay
Abstract: A simple and specific radioimmunoassay is described for measuring urinary tetrahydroaldosterone (THAldo). The antiserum used is highly sensitive to aldosterone lactone, but is sufficiently sensitive to tetrahydroaldosterone lactone when the antibody dilution is reduced from 1:500,000 to 1:20,000. Specific activities of urinary THAldo determined by the radioimmunoassay were in close agreement with values obtained by a colorimetric method (r = 0.966). Subjects treated with ACTH showed a transitory increase in THAldo excretion when THAldo was quantitated by the radioimmunoassay. These findings differ from the sustained increase in THAldo excretion in response to ACTH reported on previously by this laboratory during a time when a colorimetric method was used to measure THAldo. The radioimmunoassay appears to be more specific than the colorimetric method when steroidogenesis is increased with ACTH. The measurement of urinary THAldo excretion by radioimmunoassay serves as a useful alternative to the more time consuming colorimetric procedures.
Publication Year: 1977
Publication Date: 1977-06-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 34
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