Title: Synthesis of Steroidal Haptens in Radioimmunoassay
Abstract:In order to obtain specific antisera used for radioimmunoassay, 11α-succinoyloxy derivatives of 3β-hydroxy-5-androsten-17-one, 5-androstene-3β, 17β-diol, 17β-hydroxy-5α-androstan-3-one, and 3β-hydroxy...In order to obtain specific antisera used for radioimmunoassay, 11α-succinoyloxy derivatives of 3β-hydroxy-5-androsten-17-one, 5-androstene-3β, 17β-diol, 17β-hydroxy-5α-androstan-3-one, and 3β-hydroxy-5-pregnen-20-one (13, 14, 23, and 33) were synthesized. Each compound was coupled with bovine serum albumin by mixed anhydride technique, and the complex was immunized in rabbits. The antisera thus obtained were found to be specific for the respective parent compound, except anti-17β-hydroxy-5α-androstan-3-one antiserum which showed relatively high cross reactivity to testosterone (55.1%).Read More