Publication Information

{'id': 'https://openalex.org/W2081623409', 'doi': 'https://doi.org/10.1046/j.1432-1327.1998.2580820.x', 'title': 'Heme‐regulated eIF‐2α kinase purifies as a hemoprotein', 'display_name': 'Heme‐regulated eIF‐2α kinase purifies as a hemoprotein', 'publication_year': 1998, 'publication_date': '1998-12-01', 'ids': {'openalex': 'https://openalex.org/W2081623409', 'doi': 'https://doi.org/10.1046/j.1432-1327.1998.2580820.x', 'mag': '2081623409', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/9874252'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1046/j.1432-1327.1998.2580820.x', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4210214924', 'display_name': 'European Journal of Biochemistry', 'issn_l': '0014-2956', 'issn': ['0014-2956', '0945-5795', '1432-1033'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5026066217', 'display_name': 'Peter J. Chefalo', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210092658', 'display_name': 'Harvard–MIT Division of Health Sciences and Technology', 'ror': 'https://ror.org/00jjeh629', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I4210092658']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Peter Joseph Chefalo', 'raw_affiliation_strings': ['Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA'], 'affiliations': [{'raw_affiliation_string': 'Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA', 'institution_ids': ['https://openalex.org/I4210092658']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5104010876', 'display_name': 'Jihyun Oh', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210092658', 'display_name': 'Harvard–MIT Division of Health Sciences and Technology', 'ror': 'https://ror.org/00jjeh629', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I4210092658']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Jihyun Oh', 'raw_affiliation_strings': ['Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA'], 'affiliations': [{'raw_affiliation_string': 'Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA', 'institution_ids': ['https://openalex.org/I4210092658']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5088609466', 'display_name': 'Maryam Rafie‐Kolpin', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210092658', 'display_name': 'Harvard–MIT Division of Health Sciences and Technology', 'ror': 'https://ror.org/00jjeh629', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I4210092658']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Maryam Rafie‐Kolpin', 'raw_affiliation_strings': ['Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA'], 'affiliations': [{'raw_affiliation_string': 'Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA', 'institution_ids': ['https://openalex.org/I4210092658']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5064313970', 'display_name': 'Beki Kan', 'orcid': 'https://orcid.org/0000-0002-2738-9680'}, 'institutions': [{'id': 'https://openalex.org/I4210092658', 'display_name': 'Harvard–MIT Division of Health Sciences and Technology', 'ror': 'https://ror.org/00jjeh629', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I4210092658']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Beki Kan', 'raw_affiliation_strings': ['Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA'], 'affiliations': [{'raw_affiliation_string': 'Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA', 'institution_ids': ['https://openalex.org/I4210092658']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5111838050', 'display_name': 'Jane‐Jane Chen', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210092658', 'display_name': 'Harvard–MIT Division of Health Sciences and Technology', 'ror': 'https://ror.org/00jjeh629', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I4210092658']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Jane‐Jane Chen', 'raw_affiliation_strings': ['Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA'], 'affiliations': [{'raw_affiliation_string': 'Harvard-MIT Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts, USA', 'institution_ids': ['https://openalex.org/I4210092658']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 1.381, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 69, 'citation_normalized_percentile': {'value': 0.929053, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 94, 'max': 95}, 'biblio': {'volume': '258', 'issue': '2', 'first_page': '820', 'last_page': '830'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T12610', 'display_name': 'RNA regulation and disease', 'score': 0.9988, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T12610', 'display_name': 'RNA regulation and disease', 'score': 0.9988, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11048', 'display_name': 'Bacteriophages and microbial interactions', 'score': 0.9698, 'subfield': {'id': 'https://openalex.org/subfields/2303', 'display_name': 'Ecology'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T11150', 'display_name': 'Endoplasmic Reticulum Stress and Disease', 'score': 0.9668, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/hemeprotein', 'display_name': 'Hemeprotein', 'score': 0.8246119}, {'id': 'https://openalex.org/keywords/hemin', 'display_name': 'Hemin', 'score': 0.7889418}], 'concepts': [{'id': 'https://openalex.org/C2776217839', 'wikidata': 'https://www.wikidata.org/wiki/Q189621', 'display_name': 'Heme', 'level': 3, 'score': 0.958583}, {'id': 'https://openalex.org/C20705724', 'wikidata': 'https://www.wikidata.org/wiki/Q902628', 'display_name': 'Hemeprotein', 'level': 4, 'score': 0.8246119}, {'id': 'https://openalex.org/C2777723881', 'wikidata': 'https://www.wikidata.org/wiki/Q107315604', 'display_name': 'Hemin', 'level': 4, 'score': 0.7889418}, {'id': 'https://openalex.org/C125996951', 'wikidata': 'https://www.wikidata.org/wiki/Q412234', 'display_name': 'Globin', 'level': 3, 'score': 0.63853157}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.5903039}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.55247414}, {'id': 'https://openalex.org/C184235292', 'wikidata': 'https://www.wikidata.org/wiki/Q421851', 'display_name': 'Kinase', 'level': 2, 'score': 0.47480622}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.3786828}, {'id': 'https://openalex.org/C2778917026', 'wikidata': 'https://www.wikidata.org/wiki/Q43041', 'display_name': 'Hemoglobin', 'level': 2, 'score': 0.3617379}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.31952477}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.27223837}], 'mesh': [{'descriptor_ui': 'D006420', 'descriptor_name': 'Hemeproteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D006427', 'descriptor_name': 'Hemin', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': True}, {'descriptor_ui': 'D012156', 'descriptor_name': 'Reticulocytes', 'qualifier_ui': 'Q000201', 'qualifier_name': 'enzymology', 'is_major_topic': True}, {'descriptor_ui': 'D019892', 'descriptor_name': 'eIF-2 Kinase', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001665', 'descriptor_name': 'Binding Sites', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001665', 'descriptor_name': 'Binding Sites', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': False}, {'descriptor_ui': 'D002846', 'descriptor_name': 'Chromatography, Affinity', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002846', 'descriptor_name': 'Chromatography, Affinity', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': False}, {'descriptor_ui': 'D019281', 'descriptor_name': 'Dimerization', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015536', 'descriptor_name': 'Down-Regulation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015536', 'descriptor_name': 'Down-Regulation', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': False}, {'descriptor_ui': 'D004791', 'descriptor_name': 'Enzyme Inhibitors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004791', 'descriptor_name': 'Enzyme Inhibitors', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D005914', 'descriptor_name': 'Globins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005914', 'descriptor_name': 'Globins', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D006418', 'descriptor_name': 'Heme', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006418', 'descriptor_name': 'Heme', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D006420', 'descriptor_name': 'Hemeproteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006427', 'descriptor_name': 'Hemin', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006427', 'descriptor_name': 'Hemin', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D008970', 'descriptor_name': 'Molecular Weight', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010766', 'descriptor_name': 'Phosphorylation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011485', 'descriptor_name': 'Protein Binding', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011485', 'descriptor_name': 'Protein Binding', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': False}, {'descriptor_ui': 'D011487', 'descriptor_name': 'Protein Conformation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011817', 'descriptor_name': 'Rabbits', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D012156', 'descriptor_name': 'Reticulocytes', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013053', 'descriptor_name': 'Spectrophotometry', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D019892', 'descriptor_name': 'eIF-2 Kinase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1046/j.1432-1327.1998.2580820.x', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4210214924', 'display_name': 'European Journal of Biochemistry', 'issn_l': '0014-2956', 'issn': ['0014-2956', '0945-5795', '1432-1033'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/9874252', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 0, 'referenced_works': [], 'related_works': ['https://openalex.org/W3094284950', 'https://openalex.org/W3045001495', 'https://openalex.org/W2068520033', 'https://openalex.org/W2045429053', 'https://openalex.org/W2043591997', 'https://openalex.org/W2042191877', 'https://openalex.org/W2015889949', 'https://openalex.org/W1999009795', 'https://openalex.org/W1996303124', 'https://openalex.org/W1995200391'], 'abstract_inverted_index': {'The': [0, 134], 'regulation': [1, 35], 'of': [2, 8, 63, 86, 93, 127, 136, 147, 159, 176, 179, 186], 'protein': [3], 'synthesis': [4, 185], 'by': [5, 18, 83, 108, 162], 'the': [6, 16, 33, 38, 59, 131, 139, 145, 150, 156, 177, 183], 'availability': [7, 178], 'heme': [9, 20, 34, 48, 137, 148, 170, 180, 189], 'in': [10, 115, 130, 190], 'reticulocytes': [11], 'is': [12, 24, 45, 142, 153], 'well': [13], 'established.': [14], 'However,': [15], 'mechanism': [17], 'which': [19, 44], 'regulates': [21], 'translational': [22], 'initiation': [23], 'not': [25, 105], 'clear.': [26], 'In': [27], 'this': [28], 'study,': [29], 'we': [30], 'have': [31], 'examined': [32], 'directly': [36], 'on': [37], 'homogeneous': [39], 'heme‐regulated': [40], 'eIF‐2α': [41, 75], 'kinase': [42, 79], '(HRI),': [43], 'activated': [46], 'during': [47], 'deficiency.': [49], 'We': [50], 'found': [51], 'that': [52, 121, 167], 'HRI': [53, 69, 97, 114, 132, 160, 168], 'purified': [54, 111], 'as': [55, 173], 'a': [56, 99, 174], 'hemoprotein': [57, 64], 'with': [58, 88, 110], 'characteristic': [60], 'Soret': [61], 'band': [62], 'at': [65], '424': [66], 'nm.': [67], 'This': [68], 'was': [70, 98], 'an': [71, 89], 'active': [72], 'autokinase': [73], 'and': [74, 77, 101, 171, 188], 'kinase,': [76], 'its': [78, 102], 'activities': [80, 103], 'were': [81], 'inhibited': [82, 107], 'submicromolar': [84], 'concentrations': [85], 'hemin': [87], 'apparent': [90], 'K': [91], 'i': [92], '0.5': [94], 'μM.': [95], 'Homogeneous': [96], 'homodimer,': [100], 'could': [104], 'be': [106], 'incubation': [109], 'inactive': [112], 'K199R': [113], 'vitro': [116], '.': [117], 'Our': [118], 'results': [119, 165], 'suggest': [120], 'there': [122], 'are': [123], 'two': [124], 'distinct': [125], 'types': [126], 'heme‐binding': [128], 'sites': [129], 'homodimer.': [133], 'binding': [135, 146], 'to': [138, 149, 181], 'first': [140], 'site': [141, 152], 'stable,': [143], 'while': [144], 'second': [151], 'responsible': [154], 'for': [155], 'rapid': [157], 'downregulation': [158], 'activity': [161], 'heme.': [163], 'These': [164], 'indicate': [166], 'binds': [169], 'serves': [172], 'sensor': [175], 'coordinate': [182], 'balanced': [184], 'globins': [187], 'erythroid': [191], 'cells.': [192]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2081623409', 'counts_by_year': [{'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 2}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 3}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 4}, {'year': 2016, 'cited_by_count': 3}, {'year': 2015, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 3}, {'year': 2013, 'cited_by_count': 3}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2024-12-09T07:48:56.329464', 'created_date': '2016-06-24'}