Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2066519212', 'doi': 'https://doi.org/10.1186/1472-6750-9-76', 'title': 'cMyc increases cell number through uncoupling of cell division from cell size in CHO cells', 'display_name': 'cMyc increases cell number through uncoupling of cell division from cell size in CHO cells', 'publication_year': 2009, 'publication_date': '2009-01-01', 'ids': {'openalex': 'https://openalex.org/W2066519212', 'doi': 'https://doi.org/10.1186/1472-6750-9-76', 'mag': '2066519212', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/19735559', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/2749834'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1186/1472-6750-9-76', 'pdf_url': 'https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-9-76', 'source': {'id': 'https://openalex.org/S53489610', 'display_name': 'BMC Biotechnology', 'issn_l': '1472-6750', 'issn': ['1472-6750'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320256', 'host_organization_name': 'BioMed Central', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310320256'], 'host_organization_lineage_names': ['Springer Nature', 'BioMed Central'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-9-76', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5025781518', 'display_name': 'Darrin Kuystermans', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I100930933', 'display_name': 'University College Dublin', 'ror': 'https://ror.org/05m7pjf47', 'country_code': 'IE', 'type': 'education', 'lineage': ['https://openalex.org/I100930933']}], 'countries': ['IE'], 'is_corresponding': False, 'raw_author_name': 'Darrin Kuystermans', 'raw_affiliation_strings': ['School of Chemical and Bioprocess Engineering, University College Dublin, Delfield, Dublin 4, Ireland'], 'affiliations': [{'raw_affiliation_string': 'School of Chemical and Bioprocess Engineering, University College Dublin, Delfield, Dublin 4, Ireland', 'institution_ids': ['https://openalex.org/I100930933']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5037097859', 'display_name': 'Mohamed Al‐Rubeai', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I100930933', 'display_name': 'University College Dublin', 'ror': 'https://ror.org/05m7pjf47', 'country_code': 'IE', 'type': 'education', 'lineage': ['https://openalex.org/I100930933']}], 'countries': ['IE'], 'is_corresponding': False, 'raw_author_name': 'Mohamed Al-Rubeai', 'raw_affiliation_strings': ['School of Chemical and Bioprocess Engineering, University College Dublin, Delfield, Dublin 4, Ireland'], 'affiliations': [{'raw_affiliation_string': 'School of Chemical and Bioprocess Engineering, University College Dublin, Delfield, Dublin 4, Ireland', 'institution_ids': ['https://openalex.org/I100930933']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 1570, 'currency': 'GBP', 'value_usd': 1925, 'provenance': 'doaj'}, 'apc_paid': {'value': 1570, 'currency': 'GBP', 'value_usd': 1925, 'provenance': 'doaj'}, 'fwci': 0.93, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 33, 'citation_normalized_percentile': {'value': 0.918299, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 92, 'max': 93}, 'biblio': {'volume': '9', 'issue': '1', 'first_page': '76', 'last_page': '76'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11663', 'display_name': 'Viral Infectious Diseases and Gene Expression in Insects', 'score': 0.9997, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11663', 'display_name': 'Viral Infectious Diseases and Gene Expression in Insects', 'score': 0.9997, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10952', 'display_name': 'PI3K/AKT/mTOR signaling in cancer', 'score': 0.9959, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10169', 'display_name': 'Protein Kinase Regulation and GTPase Signaling', 'score': 0.9907, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/cell-size', 'display_name': 'Cell size', 'score': 0.6098042}], 'concepts': [{'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.92076814}, {'id': 'https://openalex.org/C1491633281', 'wikidata': 'https://www.wikidata.org/wiki/Q7868', 'display_name': 'Cell', 'level': 2, 'score': 0.6188418}, {'id': 'https://openalex.org/C3017597547', 'wikidata': 'https://www.wikidata.org/wiki/Q189159', 'display_name': 'Cell size', 'level': 2, 'score': 0.6098042}, {'id': 'https://openalex.org/C85813293', 'wikidata': 'https://www.wikidata.org/wiki/Q188909', 'display_name': 'Cell division', 'level': 3, 'score': 0.5710003}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.5680672}, {'id': 'https://openalex.org/C62112901', 'wikidata': 'https://www.wikidata.org/wiki/Q189159', 'display_name': 'Cell growth', 'level': 2, 'score': 0.47347298}, {'id': 'https://openalex.org/C175656101', 'wikidata': 'https://www.wikidata.org/wiki/Q56646', 'display_name': 'Chinese hamster ovary cell', 'level': 3, 'score': 0.45767352}, {'id': 'https://openalex.org/C81885089', 'wikidata': 'https://www.wikidata.org/wiki/Q189082', 'display_name': 'Cell culture', 'level': 2, 'score': 0.2485734}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.20875159}], 'mesh': [{'descriptor_ui': 'D002455', 'descriptor_name': 'Cell Division', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D048429', 'descriptor_name': 'Cell Size', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D016271', 'descriptor_name': 'Proto-Oncogene Proteins c-myc', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D000596', 'descriptor_name': 'Amino Acids', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D000596', 'descriptor_name': 'Amino Acids', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017209', 'descriptor_name': 'Apoptosis', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D019149', 'descriptor_name': 'Bioreactors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016466', 'descriptor_name': 'CHO Cells', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002452', 'descriptor_name': 'Cell Count', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018929', 'descriptor_name': 'Cell Culture Techniques', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D049109', 'descriptor_name': 'Cell Proliferation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006224', 'descriptor_name': 'Cricetinae', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003412', 'descriptor_name': 'Cricetulus', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003470', 'descriptor_name': 'Culture Media', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016193', 'descriptor_name': 'G1 Phase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005947', 'descriptor_name': 'Glucose', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005947', 'descriptor_name': 'Glucose', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D016271', 'descriptor_name': 'Proto-Oncogene Proteins c-myc', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016271', 'descriptor_name': 'Proto-Oncogene Proteins c-myc', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}], 'locations_count': 3, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1186/1472-6750-9-76', 'pdf_url': 'https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-9-76', 'source': {'id': 'https://openalex.org/S53489610', 'display_name': 'BMC Biotechnology', 'issn_l': '1472-6750', 'issn': ['1472-6750'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320256', 'host_organization_name': 'BioMed Central', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310320256'], 'host_organization_lineage_names': ['Springer Nature', 'BioMed Central'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2749834', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/19735559', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1186/1472-6750-9-76', 'pdf_url': 'https://bmcbiotechnol.biomedcentral.com/counter/pdf/10.1186/1472-6750-9-76', 'source': {'id': 'https://openalex.org/S53489610', 'display_name': 'BMC Biotechnology', 'issn_l': '1472-6750', 'issn': ['1472-6750'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320256', 'host_organization_name': 'BioMed Central', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310320256'], 'host_organization_lineage_names': ['Springer Nature', 'BioMed Central'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 44, 'referenced_works': ['https://openalex.org/W1220658840', 'https://openalex.org/W128824071', 'https://openalex.org/W1480318984', 'https://openalex.org/W1499999815', 'https://openalex.org/W1561952100', 'https://openalex.org/W1899319846', 'https://openalex.org/W1975162420', 'https://openalex.org/W1976697406', 'https://openalex.org/W1976874039', 'https://openalex.org/W1982085761', 'https://openalex.org/W1990822688', 'https://openalex.org/W1993646044', 'https://openalex.org/W2007298706', 'https://openalex.org/W2009886463', 'https://openalex.org/W2014735524', 'https://openalex.org/W2021505095', 'https://openalex.org/W2035545032', 'https://openalex.org/W2038310727', 'https://openalex.org/W2041968921', 'https://openalex.org/W2045899555', 'https://openalex.org/W2046528425', 'https://openalex.org/W2049627522', 'https://openalex.org/W2059262359', 'https://openalex.org/W2070329157', 'https://openalex.org/W2074670507', 'https://openalex.org/W2078511240', 'https://openalex.org/W2082416572', 'https://openalex.org/W2084508246', 'https://openalex.org/W2094222260', 'https://openalex.org/W2104014517', 'https://openalex.org/W2109475867', 'https://openalex.org/W2118540273', 'https://openalex.org/W2124743957', 'https://openalex.org/W2142627993', 'https://openalex.org/W2164461769', 'https://openalex.org/W2165426709', 'https://openalex.org/W2317461922', 'https://openalex.org/W2320732213', 'https://openalex.org/W2388163742', 'https://openalex.org/W4229926555', 'https://openalex.org/W4232896160', 'https://openalex.org/W4238475216', 'https://openalex.org/W4246039094', 'https://openalex.org/W4301327629'], 'related_works': ['https://openalex.org/W2361888356', 'https://openalex.org/W2354502831', 'https://openalex.org/W2320661246', 'https://openalex.org/W2086625401', 'https://openalex.org/W2083693850', 'https://openalex.org/W2066519212', 'https://openalex.org/W2056866400', 'https://openalex.org/W2009668940', 'https://openalex.org/W1991488085', 'https://openalex.org/W1912919312'], 'abstract_inverted_index': {'Over': [0], 'the': [1, 4, 11, 25, 33, 51, 54, 74, 95, 105, 117, 141, 152, 160, 173, 179, 202, 205, 239], 'past': [2], 'decades,': [3], 'increase': [5, 62, 107, 206, 237, 240], 'in': [6, 19, 45, 57, 69, 108, 125, 133, 193, 207, 241], 'maximal': [7, 66, 109], 'cell': [8, 34, 43, 67, 98, 101, 110, 164, 182, 187, 191, 208, 215, 233, 242], 'numbers': [9, 44], 'for': [10, 41], 'production': [12], 'of': [13, 27, 32, 38, 53, 90, 97, 115, 123, 156, 159, 172, 181, 213, 226], 'mammalian': [14], 'derived': [15], 'biologics': [16], 'has': [17], 'been': [18], 'a': [20, 81, 87, 130, 148, 211, 229], 'large': [21], 'part': [22], 'due': [23], 'to': [24, 73, 80, 94, 168], 'development': [26], 'optimal': [28], 'feeding': [29], 'strategies.': [30], 'Engineering': [31], 'line': [35, 165, 234], 'is': [36, 176], 'one': [37], 'probable': [39], 'approaches': [40], 'increasing': [42, 223], 'bioreactor.': [46], 'We': [47], 'have': [48], 'demonstrated': [49], 'that': [50, 178, 204], 'over-expression': [52], 'c-myc': [55, 163, 231], 'gene': [56], 'immortalised': [58], 'CHO': [59, 194], 'cells': [60, 118], 'can': [61], 'proliferation': [63], 'rate': [64, 200], 'and': [65, 93, 135, 185, 218], 'density': [68, 111], 'batch': [70, 195], 'culture': [71], 'compared': [72], 'control.': [75, 153, 174], 'The': [76, 154, 197], 'changes': [77], 'were': [78], 'attributed': [79], 'rapid': [82], 'transition': [83], 'into': [84], 'S-phase': [85], 'from': [86, 100, 151, 228], 'shortened': [88], 'duration': [89], 'G1': [91], 'phase': [92], 'uncoupling': [96], 'size': [99, 126], 'proliferation.': [102], 'To': [103], 'achieve': [104], '>70%': [106], 'without': [112], 'additional': [113], 'supply': [114], 'nutrients': [116], 'underwent': [119], 'an': [120], 'overall': [121], 'reduction': [122], '14%': [124], 'as': [127, 129], 'well': [128], 'significant': [131, 149], 'decrease': [132], 'glucose': [134], 'amino': [136], 'acid': [137], 'consumption': [138], 'rate.': [139], 'Consequently,': [140], 'total': [142], 'biomass': [143], 'accumulation': [144], 'did': [145, 235], 'not': [146, 236], 'show': [147], 'change': [150], 'amount': [155], 'hSEAP-hFc': [157, 227], 'activity': [158], 'over': [161], 'expressing': [162], 'was': [166, 210], 'found': [167], 'be': [169], 'within': [170], '0.7%': [171], 'It': [175], 'shown': [177], 'manipulation': [180], 'cycle': [183, 216], 'kinetics': [184, 217], 'indirectly': [186], 'metabolism': [188, 220], 'gives': [189], 'higher': [190], 'densities': [192], 'cultures.': [196], 'unaltered': [198], 'apoptotic': [199], 'supported': [201], 'proposition': [203], 'number': [209], 'result': [212], 'enhance': [214], 'cellular': [219], 'rather': [221], 'than': [222], 'viability.': [224], 'Production': [225], 'constitutive': [230], 'over-expressing': [232], 'with': [238], 'number.': [243]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2066519212', 'counts_by_year': [{'year': 2024, 'cited_by_count': 3}, {'year': 2023, 'cited_by_count': 2}, {'year': 2022, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 4}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 2}, {'year': 2016, 'cited_by_count': 2}, {'year': 2015, 'cited_by_count': 4}, {'year': 2014, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 2}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2024-12-07T17:47:36.896755', 'created_date': '2016-06-24'}