Title: Immunoblot detection of pyridoxal phosphate binding proteins in liver and hepatoma cytosolic extracts
Abstract: A monoclonal antibody, highly selective for the 5'-phosphopyridoxyl group, can be used to detect cytosolic pyridoxal-5'-phosphate binding proteins by an immunoblot procedure. This technique, when applied to sodium borohydride-treated cytosolic extracts obtained from normal rat liver at various stages of development as well as several liver-derived Morris hepatomas, reveals patterns of pyridoxal-5'-phosphate binding proteins that are characteristic of the various sources of cytosol. These findings suggest that there are developmental and tumor-specific requirements for pyridoxal-5'-phosphate, the coenzymatically active form of vitamin B-6.
Publication Year: 1983
Publication Date: 1983-04-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 6
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