Title: An improved apotryptophanase assay for pyridoxal phosphate
Abstract: An improved procedure for the estimation of pyridoxal phosphate by the apotryptophanase method is described. The method depends upon the use of a highly active apotryptophanase preparation from E. coliB1t7A. It is a colorimetric method that permits quantitative measurement of nanogram quantities of pyridoxal phosphate without the use of radioactive substrates. The procedure is suitable both for assaying enzymes whose product is pyridoxal phosphate and for the determination of pyridoxal phosphate in tissue extracts.
Publication Year: 1972
Publication Date: 1972-02-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 35
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