Title: Effects of Pyridoxal 5′-Phosphate on the Refolding of Aspartate Aminotransferase
Abstract: The effects of pyridoxal 5′-phosphate on the refolding of aspartate aminotransferase were studied. The refolding of the cytoplasmic isozyme of pig heart aspartate aminotransferase denatured with 6 m guanidine hydrochloride depends critically on the presence of pyridoxal 5′-phosphate. Pyridoxal 5′-phosphate affects a refolding intermediate at guanidine hydrochloride concentrations between 3.5 and 2.0 m to bring out the correct folding. In contrast, thermostable aspartate aminotransferase of Bacillus sp. YM-2 can be refolded independently of pyridoxal 5′-phosphate.
Publication Year: 1994
Publication Date: 1994-01-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 1
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