Title: NAD(P)H dehydrogenase (quinone) from Sporotrichum pulverulentum
Abstract: The intracellular quinone oxidoreductase system [NAD(P)H dehydrogenase (quinone), EC 1.6.99.2; NAD(P)H: quinone oxidoreductase] from Sporotrichum pulverulentum catalyzes the reduction of a quinone to the corresponding phenol using reduced pyridine nucleotides as electron donors. This chapter describes assay method of the enzyme. The enzyme is assayed by measuring the rate of decrease in absorbance at 340 nm, due to the oxidation of reduced pyridinc nucleotide, using 2-mcthoxyquinone as electron acceptor. The chapter also describes growth of the fungus and preparation of mycelial extract. In crude mycelial extracts, the enzyme is active over a broad pH range (pH 4–8) with an optimum near pH 5.6. Enzyme activity is reduced by approximately 50% when citrate buffer is replaced by phosphate.
Publication Year: 1988
Publication Date: 1988-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 10
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