Title: DNase I Footprinting as an Assay for Mammalian Gene Regulatory Proteins
Abstract:The DNase I footprinting method was first described by Galas and Schmitz (1). The technique remains unsurpassed as a way of gaining direct and immediate information about the location of a protein bin...The DNase I footprinting method was first described by Galas and Schmitz (1). The technique remains unsurpassed as a way of gaining direct and immediate information about the location of a protein binding site in the DNA sequence. In addition, the footprinting method provides a way of studying specific binding in the presence of a high nonspecific background, a common situation when working with rare proteins that have been only partially purified. So effective is the footprinting method in discriminating between specific and nonspecific binding that it is often possible to visualize specific protein-DNA interactions with entirely unfractionated protein extracts from mammalian nuclei.Read More
Publication Year: 1987
Publication Date: 1987-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
Access and Citation
Cited By Count: 53
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