Title: [22] Isolation of amyloplasts from developing endosperm of maize (Zea mays L.)
Abstract: This chapter describes a method for the isolation of amyloplasts from developing endosperm of maize. Probably the most critical stage in the isolation of intact amyloplasts from maize endosperm is the release of the amyloplasts from the cells. Amyloplasts containing a single large starch granule, such as potato and maize, is very prone to rupture during even low-gravity centrifugation. Although castor bean endosperm protoplasts were ruptured with little damage to plastids by passage through nylon mesh, the presence of large starch granules in the maize amyloplasts creates a special problem. For example, during maize endosperm protoplast rupture the starch granules quickly settle and cake on the surface of the nylon mesh. The trypsin treatment is shown to increase the rate of maize amyloplast membrane rupture and thus resulted in a low estimate of percentage intactness. Dihydroxyacetone phosphate (DHAP) readily crosses the maize amyloplast membrane and is converted to starch.
Publication Year: 1987
Publication Date: 1987-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
Access and Citation
Cited By Count: 8
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