Title: Induction, suppression, amplification of RNA silencing during viral infection
Abstract: RNA silencing is a mechanism involved in the suppression of gene expression through nucleotide sequence-specific interactions mediated by RNA. A double-stranded RNA is processed by Dicer into 21- to 24-nt RNAs, called short-interfering (si)RNA that incorporate into a RNA-Induced Silencing Complex, to guide cleavage target mRNA in a sequence-specific manner. RNA silencing plays important antiviral role in plants. In parallel, most of phytoviruses produce suppressor proteins to counteract RNA silencing. RNA silencing can be amplified through the activity of the cellular RNA-dependent RNA polymerase (RDR). We studied RNA silencing during Cauliflower mosaic virus (CaMV) infection. We found that the four Arabidopsis Dicer-like (DCLs) proteins are involved to produce two classes of viral siRNAs. Then, we analysed the interactions between five silencing suppressors and RDR6 and identified the DCLs associated to RDR6. We also showed that, at least in some cases, RDR6 uses small RNAs as primers.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: dissertation
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