Development and Validation of an Analytical RP-HPLC Method for Simultaneous Estimation of Losartan and its Active Metabolite (EXP-3174) in Isolated Perfused Rat Liver

Name: Work Video:
Duration: 3 min 30 s
Mahsa Toolabi; Reyhaneh Ramezankhani; Nadereh Rahbar; Maryam Dibaei; Alireza Foroumadi; Hoda Lavasani; Vida Kazemi; Mohammad Reza Rouini
{'id': 'https://openalex.org/W4389441084', 'doi': 'https://doi.org/10.2174/0115734129272952231103080114', 'title': 'Development and Validation of an Analytical RP-HPLC Method for Simultaneous Estimation of Losartan and its Active Metabolite (EXP-3174) in Isolated Perfused Rat Liver', 'display_name': 'Development and Validation of an Analytical RP-HPLC Method for Simultaneous Estimation of Losartan and its Active Metabolite (EXP-3174) in Isolated Perfused Rat Liver', 'publication_year': 2023, 'publication_date': '2023-12-01', 'ids': {'openalex': 'https://openalex.org/W4389441084', 'doi': 'https://doi.org/10.2174/0115734129272952231103080114'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.2174/0115734129272952231103080114', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S202974686', 'display_name': 'Current Pharmaceutical Analysis', 'issn_l': '1573-4129', 'issn': ['1573-4129', '1875-676X'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320079', 'host_organization_name': 'Bentham Science Publishers', 'host_organization_lineage': ['https://openalex.org/P4310320079'], 'host_organization_lineage_names': ['Bentham Science Publishers'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5050331087', 'display_name': 'Mahsa Toolabi', 'orcid': 'https://orcid.org/0000-0002-0551-4030'}, 'institutions': [{'id': 'https://openalex.org/I119939603', 'display_name': 'Ahvaz Jundishapur University of Medical Sciences', 'ror': 'https://ror.org/01rws6r75', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I119939603']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Mahsa Toolabi', 'raw_affiliation_strings': ['Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz,\nIran'], 'affiliations': [{'raw_affiliation_string': 'Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz,\nIran', 'institution_ids': ['https://openalex.org/I119939603']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5071241526', 'display_name': 'Reyhaneh Ramezankhani', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I119939603', 'display_name': 'Ahvaz Jundishapur University of Medical Sciences', 'ror': 'https://ror.org/01rws6r75', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I119939603']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Reyhaneh Ramezankhani', 'raw_affiliation_strings': ['Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz,\nIran'], 'affiliations': [{'raw_affiliation_string': 'Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz,\nIran', 'institution_ids': ['https://openalex.org/I119939603']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5080170394', 'display_name': 'Nadereh Rahbar', 'orcid': 'https://orcid.org/0000-0002-0663-7075'}, 'institutions': [{'id': 'https://openalex.org/I119939603', 'display_name': 'Ahvaz Jundishapur University of Medical Sciences', 'ror': 'https://ror.org/01rws6r75', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I119939603']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Nadereh Rahbar', 'raw_affiliation_strings': ['Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz,\nIran', 'Nanotechnology Research Center, Faculty of Pharmacy, Ahvaz Jundishapur\nUniversity of Medical Sciences, Ahvaz, Iran'], 'affiliations': [{'raw_affiliation_string': 'Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz,\nIran', 'institution_ids': ['https://openalex.org/I119939603']}, {'raw_affiliation_string': 'Nanotechnology Research Center, Faculty of Pharmacy, Ahvaz Jundishapur\nUniversity of Medical Sciences, Ahvaz, Iran', 'institution_ids': ['https://openalex.org/I119939603']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5028457561', 'display_name': 'Maryam Dibaei', 'orcid': 'https://orcid.org/0000-0001-6758-6518'}, 'institutions': [{'id': 'https://openalex.org/I161106909', 'display_name': 'Iran University of Medical Sciences', 'ror': 'https://ror.org/03w04rv71', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I161106909']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Maryam Dibaei', 'raw_affiliation_strings': ['Razi Drug Research Center, Iran University of Medical Sciences,\nTehran, Iran'], 'affiliations': [{'raw_affiliation_string': 'Razi Drug Research Center, Iran University of Medical Sciences,\nTehran, Iran', 'institution_ids': ['https://openalex.org/I161106909']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5010172433', 'display_name': 'Alireza Foroumadi', 'orcid': 'https://orcid.org/0000-0003-2416-5611'}, 'institutions': [{'id': 'https://openalex.org/I70640408', 'display_name': 'Tehran University of Medical Sciences', 'ror': 'https://ror.org/01c4pz451', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I70640408']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Alireza Foroumadi', 'raw_affiliation_strings': ['Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences,\nTehran, Iran'], 'affiliations': [{'raw_affiliation_string': 'Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences,\nTehran, Iran', 'institution_ids': ['https://openalex.org/I70640408']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5029392311', 'display_name': 'Hoda Lavasani', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I70640408', 'display_name': 'Tehran University of Medical Sciences', 'ror': 'https://ror.org/01c4pz451', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I70640408']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Hoda Lavasani', 'raw_affiliation_strings': ['Biopharmaceutics and Pharmacokinetic Division, Department of Pharmaceutics, Faculty of Pharmacy, Tehran\nUniversity of Medical Sciences, Tehran, Iran'], 'affiliations': [{'raw_affiliation_string': 'Biopharmaceutics and Pharmacokinetic Division, Department of Pharmaceutics, Faculty of Pharmacy, Tehran\nUniversity of Medical Sciences, Tehran, Iran', 'institution_ids': ['https://openalex.org/I70640408']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5019527954', 'display_name': 'Vida Kazemi', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210138350', 'display_name': 'Iran Nanohealth Committee Food and Drug Organization', 'ror': 'https://ror.org/03vmxkp39', 'country_code': 'IR', 'type': 'government', 'lineage': ['https://openalex.org/I4210110574', 'https://openalex.org/I4210138350']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Vida Kazemi', 'raw_affiliation_strings': ['Food and Drug Laboratory Research Center, Food and Drug Administration, MOH &amp; ME, Tehran,\nIran'], 'affiliations': [{'raw_affiliation_string': 'Food and Drug Laboratory Research Center, Food and Drug Administration, MOH &amp; ME, Tehran,\nIran', 'institution_ids': ['https://openalex.org/I4210138350']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5036971214', 'display_name': 'Mohammad Reza Rouini', 'orcid': 'https://orcid.org/0000-0003-0412-8581'}, 'institutions': [{'id': 'https://openalex.org/I70640408', 'display_name': 'Tehran University of Medical Sciences', 'ror': 'https://ror.org/01c4pz451', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I70640408']}], 'countries': ['IR'], 'is_corresponding': True, 'raw_author_name': 'Mohammadreza Rouini', 'raw_affiliation_strings': ['Biopharmaceutics and Pharmacokinetic Division, Department of Pharmaceutics, Faculty of Pharmacy, Tehran\nUniversity of Medical Sciences, Tehran, Iran'], 'affiliations': [{'raw_affiliation_string': 'Biopharmaceutics and Pharmacokinetic Division, Department of Pharmaceutics, Faculty of Pharmacy, Tehran\nUniversity of Medical Sciences, Tehran, Iran', 'institution_ids': ['https://openalex.org/I70640408']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 4, 'corresponding_author_ids': ['https://openalex.org/A5036971214'], 'corresponding_institution_ids': ['https://openalex.org/I70640408'], 'apc_list': None, 'apc_paid': None, 'fwci': 0.324, 'has_fulltext': False, 'cited_by_count': 1, 'citation_normalized_percentile': {'value': 0.522046, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 67, 'max': 78}, 'biblio': {'volume': '19', 'issue': '10', 'first_page': '786', 'last_page': '793'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11460', 'display_name': 'Analytical Methods in Pharmaceuticals', 'score': 0.9877, 'subfield': {'id': 'https://openalex.org/subfields/1602', 'display_name': 'Analytical Chemistry'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11460', 'display_name': 'Analytical Methods in Pharmaceuticals', 'score': 0.9877, 'subfield': {'id': 'https://openalex.org/subfields/1602', 'display_name': 'Analytical Chemistry'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10908', 'display_name': 'Analytical Chemistry and Chromatography', 'score': 0.9682, 'subfield': {'id': 'https://openalex.org/subfields/1607', 'display_name': 'Spectroscopy'}, 'field': {'id': 'https://openalex.org/fields/16', 'display_name': 'Chemistry'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10375', 'display_name': 'Pharmacogenetics and Drug Metabolism', 'score': 0.951, 'subfield': {'id': 'https://openalex.org/subfields/3004', 'display_name': 'Pharmacology'}, 'field': {'id': 'https://openalex.org/fields/30', 'display_name': 'Pharmacology, Toxicology and Pharmaceutics'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/active-metabolite', 'display_name': 'Active metabolite', 'score': 0.79189134}], 'concepts': [{'id': 'https://openalex.org/C2777477808', 'wikidata': 'https://www.wikidata.org/wiki/Q407595', 'display_name': 'Metabolite', 'level': 2, 'score': 0.9322628}, {'id': 'https://openalex.org/C2780288358', 'wikidata': 'https://www.wikidata.org/wiki/Q410074', 'display_name': 'Losartan', 'level': 4, 'score': 0.908358}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.8514019}, {'id': 'https://openalex.org/C149151106', 'wikidata': 'https://www.wikidata.org/wiki/Q4677569', 'display_name': 'Active metabolite', 'level': 3, 'score': 0.79189134}, {'id': 'https://openalex.org/C43617362', 'wikidata': 'https://www.wikidata.org/wiki/Q170050', 'display_name': 'Chromatography', 'level': 1, 'score': 0.7696868}, {'id': 'https://openalex.org/C179998833', 'wikidata': 'https://www.wikidata.org/wiki/Q381233', 'display_name': 'High-performance liquid chromatography', 'level': 2, 'score': 0.59693736}, {'id': 'https://openalex.org/C112705442', 'wikidata': 'https://www.wikidata.org/wiki/Q323936', 'display_name': 'Pharmacokinetics', 'level': 2, 'score': 0.44278425}, {'id': 'https://openalex.org/C98274493', 'wikidata': 'https://www.wikidata.org/wiki/Q128406', 'display_name': 'Pharmacology', 'level': 1, 'score': 0.28989774}, {'id': 'https://openalex.org/C2908929049', 'wikidata': 'https://www.wikidata.org/wiki/Q65963433', 'display_name': 'Angiotensin II', 'level': 3, 'score': 0.21050042}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.11394495}, {'id': 'https://openalex.org/C170493617', 'wikidata': 'https://www.wikidata.org/wiki/Q208467', 'display_name': 'Receptor', 'level': 2, 'score': 0.10756898}, {'id': 'https://openalex.org/C71924100', 'wikidata': 'https://www.wikidata.org/wiki/Q11190', 'display_name': 'Medicine', 'level': 0, 'score': 0.056000084}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.2174/0115734129272952231103080114', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S202974686', 'display_name': 'Current Pharmaceutical Analysis', 'issn_l': '1573-4129', 'issn': ['1573-4129', '1875-676X'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320079', 'host_organization_name': 'Bentham Science Publishers', 'host_organization_lineage': ['https://openalex.org/P4310320079'], 'host_organization_lineage_names': ['Bentham Science Publishers'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'display_name': 'Good health and well-being', 'score': 0.48, 'id': 'https://metadata.un.org/sdg/3'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 25, 'referenced_works': ['https://openalex.org/W1908994362', 'https://openalex.org/W1950014125', 'https://openalex.org/W1972683398', 'https://openalex.org/W1981658210', 'https://openalex.org/W2000468448', 'https://openalex.org/W2000747577', 'https://openalex.org/W2022126801', 'https://openalex.org/W2028901584', 'https://openalex.org/W2031490463', 'https://openalex.org/W2048760599', 'https://openalex.org/W2059144066', 'https://openalex.org/W2082409779', 'https://openalex.org/W2084504139', 'https://openalex.org/W2092818633', 'https://openalex.org/W2135375815', 'https://openalex.org/W2326493103', 'https://openalex.org/W2891675627', 'https://openalex.org/W2900167300', 'https://openalex.org/W2969292601', 'https://openalex.org/W3044842504', 'https://openalex.org/W3089914218', 'https://openalex.org/W4281715173', 'https://openalex.org/W4313445696', 'https://openalex.org/W4365443912', 'https://openalex.org/W4385488010'], 'related_works': ['https://openalex.org/W4389441084', 'https://openalex.org/W4244865049', 'https://openalex.org/W2582322678', 'https://openalex.org/W2410851926', 'https://openalex.org/W2114321573', 'https://openalex.org/W2100740897', 'https://openalex.org/W2057787401', 'https://openalex.org/W2044607430', 'https://openalex.org/W2041166445', 'https://openalex.org/W1536088879'], 'abstract_inverted_index': {'Background:': [0], 'The': [1, 21, 151, 163, 186], 'liver': [2, 19, 55, 86], 'perfusion': [3, 87], 'method': [4, 33, 76], 'is': [5], 'frequently': [6], 'used': [7, 178, 193], 'in': [8, 50, 85, 201], 'drug': [9], 'pharmacokinetic': [10], 'studies': [11], 'and': [12, 29, 45, 73, 81, 94, 121, 131, 136, 149, 153, 159, 165, 171, 181, 188, 197], 'the': [13, 40, 132, 183], 'various': [14], 'effects': [15], 'of': [16, 23, 43, 79, 110, 118], 'drugs': [17], 'on': [18], 'tissue.': [20], 'aim': [22], 'this': [24, 91], 'study': [25], 'was': [26, 64, 144, 177, 191], 'to': [27, 38, 71, 179, 194], 'establish': [28], 'validate': [30, 74], 'an': [31, 51], 'analytical': [32], 'using': [34, 101], 'high-performance': [35], 'liquid': [36], 'chromatography': [37], 'determine': [39], 'simultaneous': [41], 'concentration': [42], 'losartan': [44, 80, 93, 130, 156, 196], 'its': [46, 82, 95, 198], 'active': [47, 83, 96, 199], 'metabolite,': [48, 97], 'EXP-3174,': [49, 98], 'isolated': [52, 202], 'perfused': [53, 203], 'rat': [54, 204], 'study.': [56], 'Method:': [57], 'An': [58], 'HPLC': [59], 'system': [60], 'with': [61, 146], 'isocratic': [62], 'mode': [63], 'used.': [65], 'Various': [66], 'chromatographic': [67], 'parameters': [68], 'were': [69, 99, 134, 157, 169], 'adjusted': [70], 'develop': [72], 'a': [75, 102, 106, 115], 'for': [77, 129, 155, 167], 'determination': [78], 'metabolite': [84, 133, 168, 200], 'media.': [88], 'Results:': [89], 'In': [90], 'study,': [92], 'separated': [100], 'C18': [103], 'stationary': [104], 'phase,': [105], 'mobile': [107], 'phase': [108], 'consisting': [109], 'acetonitrile:': [111], 'phosphate': [112], 'buffer': [113], 'at': [114, 124], 'flow': [116], 'rate': [117], '1': [119], 'mL.min-1,': [120], 'UV': [122], 'detection': [123], '254': [125], 'nm.': [126], 'Retention': [127], 'times': [128], '10': [135], '16': [137], 'minutes,': [138], 'respectively.': [139, 162, 174], 'Linearity': [140], 'from': [141], '25-250': [142], 'ng.ml-1': [143], 'validated': [145, 189], 'acceptable': [147], 'accuracy': [148], 'precision.': [150], 'LOD': [152, 164], 'LOQ': [154, 166], '7.0': [158], '21.1': [160], 'ng.ml-1,': [161, 173], '7.4': [170], '22.4': [172], 'ChromGate®': [175], 'software': [176], 'acquire': [180], 'process': [182], 'data.': [184], 'Conclusions:': [185], 'optimized': [187], 'technique': [190], 'effectively': [192], 'analyze': [195], 'liver.': [205]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W4389441084', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}], 'updated_date': '2025-01-05T16:01:36.414975', 'created_date': '2023-12-08'}
Publication Information

Basic Information

Access and Citation

AI Researcher Chatbot

Primary Location

Authors

Topics

Keywords

Related Works
