Title: Assessing the influence of the inclusion of <i>Bacillus subtilis</i> <scp>AQUA‐GROW</scp> <sup>®</sup> as feed additive on the growth performance, feed utilization, immunological responses and body composition of the Pacific white shrimp, <i>Litopenaeus vannamei</i>
Abstract: Aquaculture ResearchVolume 53, Issue 18 p. 6606-6615 ORIGINAL ARTICLE Assessing the influence of the inclusion of Bacillus subtilis AQUA-GROW® as feed additive on the growth performance, feed utilization, immunological responses and body composition of the Pacific white shrimp, Litopenaeus vannamei El-Sayed Hemdan Eissa, Corresponding Author El-Sayed Hemdan Eissa [email protected] orcid.org/0000-0002-7229-2175 Fish Research Centre, Arish University, Arish, Egypt Correspondence Moaheda E.H. Eissa, Department of Aquaculture, Faculty of Fish & Fisheries Technology, Aswan University, Aswan, Egypt. Email: [email protected] Mohammad Bodrul Munir, Aquatic Science Program, FSTS, UNIMAS, Kota Samarahan, Sarawak, Malaysia. Email: [email protected] El-Sayed Hemdan Eissa, Fish Research Centre, Arish University, Arish, Egypt. Email: [email protected]Search for more papers by this authorNorhan H. Ahmed, Norhan H. Ahmed Department of Animal production, Faculty of Agriculture, Zagazig University, Zagazig, EgyptSearch for more papers by this authorAshraf A. El-Badawi, Ashraf A. El-Badawi Central Laboratory for Aquaculture Research, Abbassa, Abo- Hammad, Sharkia, Egypt Biology Department, University College, Umm-Al Qura University, Makkah, Saudi ArabiaSearch for more papers by this authorMohammad Bodrul Munir, Corresponding Author Mohammad Bodrul Munir [email protected] orcid.org/0000-0003-1162-1702 Aquatic Science Program, FSTS, UNIMAS, Kota Samarahan, Sarawak, Malaysia Correspondence Moaheda E.H. Eissa, Department of Aquaculture, Faculty of Fish & Fisheries Technology, Aswan University, Aswan, Egypt. Email: [email protected] Mohammad Bodrul Munir, Aquatic Science Program, FSTS, UNIMAS, Kota Samarahan, Sarawak, Malaysia. Email: [email protected] El-Sayed Hemdan Eissa, Fish Research Centre, Arish University, Arish, Egypt. Email: [email protected]Search for more papers by this authorOmayma M. Abd Al-Kareem, Omayma M. Abd Al-Kareem Department of Fish Health and Diseases, Aswan University, Aswan, EgyptSearch for more papers by this authorMoaheda E. H. Eissa, Corresponding Author Moaheda E. H. Eissa [email protected] orcid.org/0000-0002-1733-6892 Department of Aquaculture, Faculty of Fish & Fisheries Technology, Aswan University, Aswan, Egypt Correspondence Moaheda E.H. Eissa, Department of Aquaculture, Faculty of Fish & Fisheries Technology, Aswan University, Aswan, Egypt. Email: [email protected] Mohammad Bodrul Munir, Aquatic Science Program, FSTS, UNIMAS, Kota Samarahan, Sarawak, Malaysia. Email: [email protected] El-Sayed Hemdan Eissa, Fish Research Centre, Arish University, Arish, Egypt. Email: [email protected]Search for more papers by this authorElamier H. M. Hussien, Elamier H. M. Hussien Zoology Department, Faculty of Science, South Valley University, Qena, EgyptSearch for more papers by this authorSalah El-Sayed Sakr, Salah El-Sayed Sakr Fish Resources and Aquaculture Department, Faculty of Agricultural Environmental Sciences, Arish University, Arish, EgyptSearch for more papers by this author El-Sayed Hemdan Eissa, Corresponding Author El-Sayed Hemdan Eissa [email protected] orcid.org/0000-0002-7229-2175 Fish Research Centre, Arish University, Arish, Egypt Correspondence Moaheda E.H. Eissa, Department of Aquaculture, Faculty of Fish & Fisheries Technology, Aswan University, Aswan, Egypt. Email: [email protected] Mohammad Bodrul Munir, Aquatic Science Program, FSTS, UNIMAS, Kota Samarahan, Sarawak, Malaysia. Email: [email protected] El-Sayed Hemdan Eissa, Fish Research Centre, Arish University, Arish, Egypt. Email: [email protected]Search for more papers by this authorNorhan H. Ahmed, Norhan H. Ahmed Department of Animal production, Faculty of Agriculture, Zagazig University, Zagazig, EgyptSearch for more papers by this authorAshraf A. El-Badawi, Ashraf A. El-Badawi Central Laboratory for Aquaculture Research, Abbassa, Abo- Hammad, Sharkia, Egypt Biology Department, University College, Umm-Al Qura University, Makkah, Saudi ArabiaSearch for more papers by this authorMohammad Bodrul Munir, Corresponding Author Mohammad Bodrul Munir [email protected] orcid.org/0000-0003-1162-1702 Aquatic Science Program, FSTS, UNIMAS, Kota Samarahan, Sarawak, Malaysia Correspondence Moaheda E.H. Eissa, Department of Aquaculture, Faculty of Fish & Fisheries Technology, Aswan University, Aswan, Egypt. Email: [email protected] Mohammad Bodrul Munir, Aquatic Science Program, FSTS, UNIMAS, Kota Samarahan, Sarawak, Malaysia. Email: [email protected] El-Sayed Hemdan Eissa, Fish Research Centre, Arish University, Arish, Egypt. Email: [email protected]Search for more papers by this authorOmayma M. Abd Al-Kareem, Omayma M. Abd Al-Kareem Department of Fish Health and Diseases, Aswan University, Aswan, EgyptSearch for more papers by this authorMoaheda E. H. Eissa, Corresponding Author Moaheda E. H. Eissa [email protected] orcid.org/0000-0002-1733-6892 Department of Aquaculture, Faculty of Fish & Fisheries Technology, Aswan University, Aswan, Egypt Correspondence Moaheda E.H. Eissa, Department of Aquaculture, Faculty of Fish & Fisheries Technology, Aswan University, Aswan, Egypt. Email: [email protected] Mohammad Bodrul Munir, Aquatic Science Program, FSTS, UNIMAS, Kota Samarahan, Sarawak, Malaysia. Email: [email protected] El-Sayed Hemdan Eissa, Fish Research Centre, Arish University, Arish, Egypt. Email: [email protected]Search for more papers by this authorElamier H. M. Hussien, Elamier H. M. Hussien Zoology Department, Faculty of Science, South Valley University, Qena, EgyptSearch for more papers by this authorSalah El-Sayed Sakr, Salah El-Sayed Sakr Fish Resources and Aquaculture Department, Faculty of Agricultural Environmental Sciences, Arish University, Arish, EgyptSearch for more papers by this author First published: 06 October 2022 https://doi.org/10.1111/are.16129Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract This study was carried out to determine the influence of four concentrations of dietary Bacillus subtilis on the growth performance, nutrient utilization, immunity and antioxidants activities, mortality rate and chemical composition of Litopenaeus vannamei. Three hundred and sixty L. vannamei (4.00 g ± 0.03) were raised in 12 hapas (1 m3 each, placed in 4 ponds) equally distributed on apparently 38.5% protein and 10.5% lipid containing three concentrations (0.5, 1.0 and 1.5) g/kg of dietary B. subtilis and a non-supplemented control in triplicates. After 56 days of feeding trial, the growth performance, feed utilization, survival rate and immune parameters were significantly (p < 0.05) better in shrimp fed with dietary B.subtilis compared to the control. The higher dose of dietary B.subtilis showed the good performance of the parameters studied, but in some instances (specific growth rate, feed conversion rate, lysosomal enzyme activity, phenoloxidase activity etc.) were not significant (p < 0.05) difference. This study indicates that dietary B. subtilis based diets have a more positive influence on the growth performance, feed utilization, survival rate and immune response of L. vannamei fingerlings. CONFLICT OF INTEREST The authors declare no competing interest. Open Research DATA AVAILABILITY STATEMENT Data availabilityData of the present article are available under request. REFERENCES Abdel-Aziz, M., Bessat, M., Fadel, A., & Elblehi, S. (2020). Responses of dietary supplementation of probiotic effective microorganisms (EMs) in Oreochromis niloticus on growth, hematological, intestinal histopathological, and antiparasitic activities. Aquaculture International., 28, 1– 17. Adorian, T. J., Jamali, H., Farsani, H. 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