Title: Secretions released from mesenchymal stem cells improve spermatogenesis restoration of cytotoxic treatment with busulfan in azoospermia mice
Abstract: AndrologiaVolume 53, Issue 8 e14144 ORIGINAL ARTICLE Secretions released from mesenchymal stem cells improve spermatogenesis restoration of cytotoxic treatment with busulfan in azoospermia mice Yi-ting Cai, Corresponding Author Yi-ting Cai [email protected] orcid.org/0000-0003-0791-2873 Department of Reproductive Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China Correspondence Yi-ting Cai, Department of Reproductive Medicine, The second affiliated hospital of Zhejiang University School of Medicine, Hangzhou, China. Jiefang road 88, Hangzhou 310000, China. Email: [email protected]Search for more papers by this authorCheng-liang Xiong, Cheng-liang Xiong Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSearch for more papers by this authorTian-shu Liu, Tian-shu Liu Department of Thoracic Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaSearch for more papers by this authorShi-liang Shen, Shi-liang Shen Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSearch for more papers by this authorJin-peng Rao, Jin-peng Rao Department of Reproductive Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaSearch for more papers by this authorFeng Qiu, Feng Qiu Department of Reproductive Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaSearch for more papers by this author Yi-ting Cai, Corresponding Author Yi-ting Cai [email protected] orcid.org/0000-0003-0791-2873 Department of Reproductive Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China Correspondence Yi-ting Cai, Department of Reproductive Medicine, The second affiliated hospital of Zhejiang University School of Medicine, Hangzhou, China. Jiefang road 88, Hangzhou 310000, China. Email: [email protected]Search for more papers by this authorCheng-liang Xiong, Cheng-liang Xiong Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSearch for more papers by this authorTian-shu Liu, Tian-shu Liu Department of Thoracic Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaSearch for more papers by this authorShi-liang Shen, Shi-liang Shen Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSearch for more papers by this authorJin-peng Rao, Jin-peng Rao Department of Reproductive Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaSearch for more papers by this authorFeng Qiu, Feng Qiu Department of Reproductive Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaSearch for more papers by this author First published: 18 June 2021 https://doi.org/10.1111/and.14144Citations: 1 Yi-ting Cai and Cheng-liang Xiong contributed equally to this work and should be considered as co-first authors. Funding information This work was supported by grants from the National Natural Science Foundation of China (Grant No. 81601268). Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract This study aimed at the efficacy of sequential treatment of bone marrow-derived mesenchymal stem cell secretion for busulfan-treated azoospermia in mice. The conditioned media (CM) was obtained from bone marrow mesenchymal stem cells (MSCs) or 293 cells. Chemically induced azoospermia mice received 200 μl MSC-CM or 293-CM twice a week intravenously for three consecutive weeks. The histological assessment of spermatogenic recovery quantifying the expression of meiosis-associated genes, and Sertoli cell barrier functional factors were assessed. The characteristics of TM4 cells (Sertoli cell line) after pre-incubation of MSC-CM in vitro were also obtained. The MSC-CM group had the most spermatogenic colonies among the three groups (p < .05), but no spermatids were seen. Expressions of the meiosis-associated genes Dazl, Vasa, Miwi, Stra8, CyclinA1, Pgk2 and Scp3 in MSC-CM testis were remarkably higher compared with 293-CM and busulfan groups respectively (p < .05). The levels of Sertoli cell barrier functional factors, for example ICAM-1 and N-cadherin, were significantly increased during MSC-CM treatment (p < .05). Moreover, pre-incubation of MSC-CM particularly accelerated the CD54 (ICAM-1) and CD44 expressions of TM4 cells and promoted cell inherent adhesion. MSC-CM treatment can significantly improve the short-term restoration of spermatogonial structures of chemically induced azoospermia related to facilitating Sertoli cell adhesion integrity. CONFLICT OF INTEREST The authors claim no conflict of interest. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available from the corresponding author upon reasonable request. Citing Literature Supporting Information Filename Description and14144-sup-0001-FigS1-S2.docxWord document, 870.1 KB Fig S1-S2 and14144-sup-0002-FigS2.tifTIFF image, 429.4 KB Fig S2 and14144-sup-0003-TableS1.docxWord document, 16.4 KB Table S1 and14144-sup-0004-Video.mp4MPEG-4 video, 5.4 MB Supplementary Material Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. 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