Title: The effects of a novel aminosteroid on WEHI-3B leukemia cells
Abstract:Objective: To evaluate the effect of a novel aminosteroid(KH) on WEHI 3B cells. Methods: The effects and mechanism of KH on proliferation, differentiation and apoptosis of WEHI 3B cells in vitro were ...Objective: To evaluate the effect of a novel aminosteroid(KH) on WEHI 3B cells. Methods: The effects and mechanism of KH on proliferation, differentiation and apoptosis of WEHI 3B cells in vitro were studied by semi solid colony culture, MTT assay, morphologic examination, NBT reduction test, NSE assay, DNA fragmentation in gel electrophoresis and RT PCR of c myc oncogene. Results: The growth of leukemic colony was inhibited by KH(10 -8 ~10 -4 ?mol·L -1 ) after treatment of KH for 7 days. The percentages of NBT and NSE positive cells were increased from 40.38% to 71.17% and 48.25% to 79.25%, respectively as well as the typical ladders of DNA fragments in gel electrophoresis were also observed after WEHI 3B cells were treated with KH(10 -8 ~10 -4 ?mol·L -1 ) for 5 days. The c myc mRNA expression of WEHI 3B cells was decreased by 58.7% after WEHI 3B cells were treated with KH(10 -8 ?mol·L -1 ) for 5 days. Conclusion: KH might suppress the proliferation of WEHI 3B cells, induce the differentiation of WEHI 3B cells into macrophage like cells and facilitate the cell apoptosis. There is relationship between the above effects and c myc mRNA expression of WEHI 3B cells. [Read More
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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