Title: Expression and clinical significance of caspase-3、survivin and k-ras in non-small cell lung cancer
Abstract: Background and objective The prognosis of non-small cell lung cancer (NSCLC) is related to many factors.Apoptosis-related genes play an important role in occurrence and development of tumors.The sig- nificance of apoptosis as a biological and prognostic factor has not been clearly established in NSCLC.The aim of the study is to evaluate the relationship between expression of caspase-3 ,survivin ,k-ras protein and the relationship between the expression of above proteins in NSCLC and theirs clinicopathological characteristics and prognostic sig- nificance and their potential relevance existing in NSCLC.Methods Caspase-3 ,survivin,k-ras protein expression were detected in NSCLC tissues and normal lung tissues adjacent to carcinomas Archival formalin fixed,paraffin embedded specimens from 91 patients who had undergone radical surgery by streptavidin-peroxidase immunohisto- chemistry.Results The positive rate of caspase-3 was significantly lower in NSCLC than in normal lung tissues adjacent to carcinomas;The positive rate of survivin,k-ras was significantly higher in NSCLC than in normal lung tissues adjacent to carcinomas(P0.05).The positive rate of caspase-3 was related to the age,histological type, TNM stage,lymph node metastasia and differentiation(P0.05),but not related to tumor size,smoke(P0.05);the positive rate of survivin was related to the age,TNM stage ,lymph node metastasia and differentiation(P0.05),but not related to histologic type ,tumor size,smoke(P0.05);the positive rate of k-ras was related to the histologic type and smoke(P0.05),but not related the age,TNM stage,lymph node metastasia,differentiation ,tumor size(P0.05). Expression of easpase-3 was negatively correlated with survivin(r=-0.662,P0.001).The multivariate analysis with Cox hazard model,survivin,caspase-3,histologic type,differentiation,TNM stage could be indicators for the prognosis of patients with NSCLC(P0.05).Conclusion There are over-expressions of survivin,k-ras protein in NSCLC and both are significantly higher than those in the normal tissue.However,caspase-3 presents the reverse result;Higher expression of survivin,lower expression of caspase-3 might be the adverse prognostic index for sur- vival in resected NSCLC patients,which indicate they might act synergistically in apoptosis of NSCLC;the relation- ship between the expression of k-ras and the prognosis of NSCLC is still not clear.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot