Abstract: Manihot esculenta, Crantz (Cassava) peel was taken for the isolation of acidic amylases. The enzyme activity of 20% and 40% precipitate was found to be 0.02 and 0.18μmole/min, whereas, 50% precipitate did not show any enzyme activity. The fractionation of 40% precipitate on Sephadex G-75 column yielded two peaks with enzyme activity 0.02 and 0.08μmole/min respectively. The second peak indicated a rich source of amylase. A gradual increase in the enzyme activity was observed at 35C to 50C, along with drastic decrease at 50C onwards. A maximum activity was observed from 50C at pH 6.8 to 7.0. The activity of purified enzyme was 0.14μmole/min.Besides, substrate concentration between 1 to 3% which led to substantial increase in enzyme activity. Further, the enzyme activity of Cassava amylase against commercial starch was tested and found that, the enzyme activity increased with increase in time and remains constant after 80 min, whereas, on its own starch there was no activity till 30 min and after this time point, the activity gradually increases and it remains constant after 90 minutes. Thus, indicating Cassava acidic amylases act very rapidly on commercial starch than on its own starch.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot