Title: Activity and Stability of Purified Amylase Produced by
Abstract: The effects of pH values, temeprature and some elements on the amylolytic activity and stability of the purified S. aureofaciens 77 amylase were studied in this investiga- tion. The purified enzyme showed its maximum activity at pH 6 within 8 rain incubation at 40~ None of the tested 6 metals showed on stimulatory effect on the enzymatic activity, Fe + ++,Cu + +and Hg ++ at high dose inhibited the enzyme activity to great extent as com- pared with Zn + +, Mn + + and Fe § + which gave less effect in this respect. The enzyme liquor was found to be thermolabile, since it lost completely its activity after 4 days incubation under room temperature and showed maximum activity during this period as a result of additions of Ca + + and NaC1. Gradual reduction was however recorded until activity reach- ed 30~ after 60 days of incubation.
Publication Year: 1990
Publication Date: 1990-01-01
Language: en
Type: article
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