Title: A Study Cultivating on Limbal Corneal Epithelial Cells of Rabbit and the Growth Characteristics in Vitro
Abstract: Objective:To establish a method for culturing limbal corneal epithelial cells of rabbit and to observe the growth characteristics of the cultured cells in vitro. Methods:Limbal corneal epithelial cells of rabbit were planted in 6 w ell culture plate and the characteristics of the cultured cells were observed by inverted phase contract microscope. The cultured cells were identified by HE, P AS, immunohistochemical staining and electron mocroscopy. Results:Limbal corneal epithelial cells of rabbit could be successfu ll y cultured. Most of the cells attached to the plastic surface of the wells durin g the first 48 hour′s culture. The cells grew prosperously at the 5th day and form ed a monolayer about in 15 days. In subculture, the cells attached to the surfac e during the first 24 hour′s culture and formed a monolayer in 10 days. There we re multi angles and a lot of microvilli on their surface and desmosomes connected betw een the cultured cells. The cultured cells were positive for cytokeratin AE1 mo noclone antibody and negative for the PAS. Conclusion: The limbal corneal epithelial cells of rabbit could be s uccessfully cultured in vitro and the cultured cells have the same characteristi cs of corneal epithelial cells of normal rabbit.
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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