Abstract: Objective To explore a novel,simple method for culturing rabbit corneal stromal cells.Methods Fresh rabbits' corneas were obtained,and the epithelium,descemet's membrane and endothelium were removed.The stroma slices after treatment were cultured in 6-well culture plate,and treatment with suspended culture(suspended stroma culture technique),tissue adherence culture technique,type Ⅱ collagenase digestion,respectively.The biological characteristics of the cultured cells in vitro were observed,and these cells were identified by vimentin immunostaining.Results Rabbit corneal stromal cells were successfully cultured with suspended stroma culture technique,with numbers of apophysis at different lengths,with spindle shape,irregular,triangle or fusiform.Nucleus were with ellipse shape and at center.Cytoplasm was clear and without other kinds of cells.Cells were with adherence after suspended culture for 8 days to 10 days and with fusion at 5 days later.The biological characteristics of suspended stromal culture technique were similar with those cultured by traditional culture techniques or type Ⅱ collagenase digestion.Cultured cells were positive with vimentin immunostaining by three methods.Conclusions The suspended stroma culture technique for culturing rabbit corneal stromal cells has many obvious advantages,such as simple,reliable,pollution-proof and highly successful rate.It provides a new way for culturing corneal stromal cells and needs to be popularized.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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