Title: HPLC based estimation and extraction of rutin, quercetin and gallic acid in Moringa oleifera plants grown in Saudi Arabia
Abstract: Moringa oleifera L. (Moringaceae) is a valuable medicinal plant with a high nutritional and industrial value. To
analyse the major polyphenolic compounds rutin, quercetin and gallic acid present in the M. oleifera l eaves, HPLC
methods have been used. In the range of 10-30 µg for rutin, quercetin and gallic acid with determinati on coefficient
(R2
) good linearity was observed and linear regression y = 1232.9x + 106.05, y = 133.53x + 202 and y = 61 9.4x +
3442.5 were achieved in 0.98, 0.99 and 1.04 min with the retention time respectively. The LOQs for rutin, quercetin
and gallic acid; 3.4, 10.6 and 4.4 µg/ml and LODs ?4.0 µg/ml for all standards were achieved. The rutin
(555.6±1.79 µg/g), quercetin (37.54±1.07µg/g) and g allic acid (48.5±1.34 µg/g) were recorded in M. ole fera. Rutin
was the most abundant flavonoid analysed in M. oleifera
Publication Year: 2016
Publication Date: 2016-01-01
Language: en
Type: article
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Cited By Count: 7
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