Title: Influence of fluoride on osteoprotegerin mRNA and protein expression in rat osteoblasts: an in vitro study
Abstract: Objective To study the influence of fluoride on the expression of osteoprotegerin(OPG) mRNA and protein in suckling rat osteoblasts. Methods Osteoblasts obtained from calvarial of suckling Wistar rats were cultured in vitro in the media supplemented with NaF at a series of doses[O(control), 1,2 and 4 mg/L groups], and OPG mRNA expression and protein were evaluated by RT-PCR and ELISA methods, respectively. Results OPG mRNA expression in suckling rat osteoblasts cultured in vitro significantly increased after exposure to NaF for 48 h and 72 h(F=333.48,808.34,P 0.05). Conclusions The results suggested that NaF could increase OPG mRNA and protein expression in suckling rat osteoblasts with a synergistic effect between the doses and exposure time.
Key words:
Osteoprotegerin; Osteoblast; Fluorides; Reverse transcription polymerase chain reaction; Enzyme-linked immunosorbent assay
Publication Year: 2009
Publication Date: 2009-03-20
Language: en
Type: article
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Cited By Count: 1
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