Title: Regulation of osteoprotegerin mRNA levels by dexamethasone in osteoblasts
Abstract: Objective To study the regulation of esteoprotegerin (OPG) mRNA expression by glucocorticoid in osteoblasts and elucidate the mechanism of glucocorticoid\|induced bone resorption. Methods Primary osteoblastic cells from calvaria of newborn SD rats were cultured, and treated with dexamethasone according to the protocol. Total cellular ribonucleic acid was isolated by the TRIzol method. Semiquantitative reverse transcriptase PCR was used to screen the level of OPG mRNA. Results Dexamethasome down\|regulated the mRNA levels of OPG dose\|and time\|dependently. After 6 hours of stimulation with 50 ng/ml dexamethasone, OPG mRNA levels significantly decreased. The inhibitory effect was seen at and above 10 ng/ml of dexamethasone. Conclusion The inhibition of OPG expression in osteoblasts may be involved in such a mechanism of glucocorticoid\|induced bone resorption and may also play a part in glucocorticoid\|induced osteoporosis.\;
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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