Title: Expression of vascular endothelial growth factor-C protein and mRNA in gastric carcinoma and its relationship with lymph node micrometastases
Abstract: Objective
To explore the expression of vascular endothelial growth factor-C (VEGF-C) protein and mRNA in gastric cancer and its relationship with lymph node micrometastases.
Methods
Immunohistochemistry and RT PCR were used to detect the expression of VEGF-C protein and mRNA in gastric carcinoma tissue, adjacent tissue and normal tissue from 80 patients who suffered from gastric carcinoma. The expression level of VEGF-C protein and mRNA were compared between tissues with positive or negative lymph node micrometastases.
Results
The positive expression rates of VEGF-C protein and mRNA in gastric cancer tissue were significantly higher than those in adjacent tissue and normal tissue (P<0.05) , which had no relationship with the patients' age, sex, tumor size, location and distant metastasis. The expression of VEGF-C was positively correlated with lymph node metastasis (P<0.01) , and that of VEGF-C mRNA with micrometastasis was closer than that of protein.
Conclusions
VEGF-C protein and mRNA in gastric cancer tissue is correlated with lymph node micrometastases, and detection of VEGF-C mRNA is prior to detection of protein. VEGF-C mRNA can be serving as a more accurate indicator of lymph node micrometastases in the gastric cancer patients.
Key words:
Gastric cancer; Vascular endothelial growth factor-C; mRNA; Lymph node micrometastases
Publication Year: 2014
Publication Date: 2014-10-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot