Title: The research of NS-398 combined with interleukin-6 antibody inhibits the proliferation of hilar cholangiocarcinoma cell line QBC939 and decreases the level of prostaglandin E2
Abstract: Objective To examine the synergistic effect of NS-398 [a specific cyclooxygenase-2 (COX-2) inhibitor] and intedeukin-6 (IL-6) antibody on proliferation inhibition and prostaglandin E2(PGE2) levels of Hilar cholangiocarcinoma (HCCA) cell line QBC939.Methods QBC939 cells were divided into 25,50 and 100 μmol/L NS-398 groups.0.1,1.0,10.0 mg/L IL-6 antibody groups and NS-398 100 μmol/L + IL-6 antibody 10 mg/L combination groups.The cells were treated for 24,48,72 h.The proliferation-inhibitory activity of QBC939 cells were detected by cell counting kit-8 (CCK-8).The PGE2 levels in the supernatant of cultured cells were quantitated by ELISA.Results After treated with NS-398 and IL-6 antibody respectively or in combination,cells proliferation were obviously inhibited,and the role in combined treatment groups was more significant than monotherapy groups compared with the negative control group (P<0.05).ELISA showed that:After 72 h,the PGE2 value of NS-398 100 μmol/L group,IL-6 antibody 10 mg/L group and NS-398 100 μmol/L + IL-6 antibody 10 mg/L group were (25.606 ± 5.676) ng/well,(36.454 ± 6.444) ng/well,(18.223 ± 4.168) ng/well and respective-ly compared with the negative control group (106.781 ± 12.423) ng/well (P < 0.05).Conclusion NS-398 and IL-6 antibody had inhibitory effects on the growth of HCCA cell line QBC939,and they could inhibit the expressions of PGE2 in QBC939 cells in a dose and time dependent manner.And the combination inhibitory effect was more apparent.
Key words:
Cholangiocarcinoma; Tumor; Cyclooxygenase-2 ; Interleukin-6 ; Prostaglandin E2
Publication Year: 2014
Publication Date: 2014-07-08
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot