Title: A correlative study of tumor necrosis factor-α in ischemic brain tissue and brain edema after cerebral ischemia-reperfusion in rats
Abstract: Objective To investigate the relationship between tumor necrosis factor-α (TNF-α) in ischemic brain tissue and bran edema after cerebral ischemia-reperfusion in rats. Methods Eighty four male SD rats were randomly assigned to either a cerebral ischemiareperfusion group (n = 44) or a sham-operation group (n = 40). A model of middle cerebral artery occlusion for 120 minutes followed by reperfusion was induced in rats using the suture method. The infarct size was determined by 2, 3, 5-triphenyi terazoloride (TTC) staining at 6 h, 24 h, 3 d, and 7 d respectively after the reperfusion. Dry-wet weight method was used to measure brain water content and evaluate the extent of brain edema, The enzyme-linked immunosorbent assay (ELISA) was used to detect the concentration of TNF-α in ischemic brain tissue. Results TNF-α level in ischemic brain tissue was increased at 6 h (445.8 ± 91.7 pg/ml) after the reperfusion, and reached the peak at day 3 (715.5 ± 121.3 pg/ml). There were significant differences compared to the sham-operation group and other time points (all P 〈 0.001 ). After that, it was decreased gradually, but it was still higher than that in the shamoperation group at day 7 (478.1 ± 145.5 pg/ml vs. 148.5 ± 101.7 pg/ml, P 〈0. 005). ]he initial change of the water content in brain tissue lagged behind the increased TNF-α. It did not increase significantly until 24 h after cerebral ischemia-reperfusion (P 〈 0.001 ). It reached the peak at day 3 (P 〈 0.001 ), and it was still higher than that in the control group at day 7 (P 〈 0.05). The evolution of cerebral infarct volume was in accordance with the changes of TNF-α level. Conclusions TNF-α is associated with the changes of brain edema and infarct volume, and it is harmful to brain tissue.
Key words:
brain ischemia; reperfusion injury; tumor necrosis factor-α; brain edema; rats
Publication Year: 2009
Publication Date: 2009-11-15
Language: en
Type: article
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