Title: Analysis of pathogens and genetic characteristics of the first case of mink breeder infected with brucellosis in Hebei Province
Abstract:Objective
A suspected Brucella strain, isolated from the first case of mink breeder in Hebei Province was identified and genetic characteristics were analyzed.
Methods
Blood sample of the patie...Objective
A suspected Brucella strain, isolated from the first case of mink breeder in Hebei Province was identified and genetic characteristics were analyzed.
Methods
Blood sample of the patient with brucellosis was collected at brucellosis laboratory of Hebei Provincial Center for Disease Control and Prevention; anti-Brucella antibody was tested and Brucella was isolated by two phase culture bottles. Conventional methods were used to identify the isolated strain, the genetic characteristics were analyzed by multiple-locus variable-number tandem-repeat analysis (MLVA).
Results
Anti-Brucella antibody was tested positive. The isolated strain was identified as Brucella melitensis biovar 1 using the conventional methods. Using Pannel 1, the strain was genotype 42 clustering to the East MediterraneanBrucella Melitensis group. It was closely clustered with Brucella melitensis biovar 3, and difference of repeated numbers at variable-number tandem-repeat (VNTR) loci bruce19 was also displayed.
Conclusion
The case is the first brucellosis case of mink breeder in Hebei Province, the isolated strain from this patient is identified as Brucella melitensis biovar 1 and the genetic characteristics of the strain are closely related to those of Brucella melitensis biovar 3.
Key words:
Brucellosis; Multiple-locus variable-number tandem-repeat analysis; Inheritant characteristicRead More
Publication Year: 2015
Publication Date: 2015-12-20
Language: en
Type: article
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