Title: Expression of IL-17 and effects of dexamethasone in rats with acute lung injury induced by lipopolysaccharide
Abstract: Objective To investigate the expression of IL-17 and the effects of low-dose dexamethasone (1 mg/kg) in rats with acute lung injury induced by LPS.Methods Fort(y)-eight adult healthy SD rats were randomly divided into three groups: normal controls group(group C),acute lung injury induced by LPS group (group ALI) and dexamethasone group (group D).To form ALI model in rats,5 mg/kg of LPS were injected into rats by intraperitoneal.Immediately 1 mg/kg of dexamethasone were injected into rats by intraperitoneal to form group D.At 2 h,6 h two time points,8 rats from each group were killed.Both left BALF and right lung tissue were obtained to measure wet to dry weight ratio (W/D),the levels of IL-17 and TNF-α in BALF by ELISA,and the histopathology was aslo performed.Results ① Lung wet/dry weight ratio and TNF-α were significantly elevated in group ALI,and the difference of each time point was statistically significant (P < 0.05) compared with group C.The histopathology had the typical ALI finds,as pulmonary edema,infiltration of inflammatory cells in lung tissues,pulmonary hemorrhage,the model of Rat ALI was successful.②Compared with group C,the BALF content of IL-17 had no significant difference at 2 h in group ALI,but it increased sharply at 6 h,the difference between two groups was statistically significant (P <0.05).③Compared with group ALI,the dexamethsone decreased sharply the expression of IL-17,the difference between two groups was statistically significant (P < 0.05).Conclusions IL-17 is involved in Rat ALI process induced by lipopolysaccharide,and it expresses significantly at 6 h.Early application of small dose dexamethasone reduces the BALF expression of IL-17 in rat model of ALI.
Key words:
Interleukin-17; Acute lung injury; Lipopolysaccharide; Dexamethsone
Publication Year: 2013
Publication Date: 2013-02-05
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot