Title: Effect of controlled lowCVP on hepatic ischemia-reperfusion injury in rabbits
Abstract: :Objective To investigatethe effect of controlled low CVP(CL-CVP)on blood flow,liver enzyme and ultrastructure ofliver after hepatic ischemia-reperfusion(I/R)injury in rabbits.Methods Thirty-two healthyNew Zealand white rabbits of both sexes weighing 1.9-2.2 kg were randomly divided into 4groups(n=8each): group Ⅰ control(group C); group ⅡCL-CVP; groupⅢ I/R and group Ⅳ CL-CVP+I/R.The animals were anesthetized with iv 3% pentobarbital30 mg/kg,tracheostomized and mechanically ventilated.In group Ⅱ and Ⅳ CVP was reduced to and maintained at4-5 cm H_2O by intrnglycerin infusion at 10-30 μg·kg~(-1)·min~(-1)while MAP was maintained ≥90 mm Hg by dopamine infusion at 30-40μg·kg~(-1)·min~(-1).In group Ⅲ and Ⅳ hepatic ischemia was produced byoccluding portal vein and hepatic artery for 30 rain followed by reperfusion.Blood flowvelocity of hepatic artery and vein and blood ALT and AST activities were measured beforeinduction of low CVP,at 0,30 min,1,2,4 and 6 h of reperfusion.Results(1)Low CVP did notaffect blood flow velocity of hepatic artery and vein.Blood flow velocity of hepaticartery was reduced while that of hepatic vein was increased by I/R.Blood flow velocity ofhepatic artery and vein was significantly increased in group Ⅳ(group CL-CVP+I/R)as comparedwith group Ⅲ(groupI/R).(2)Blood ALT and AST activities were not affected by low CVP but significantincreased by hepatic I/R.Low CVP significantly attenuated I/R-induced increase in AST andALT activities.(3)Low CVP significantly ameliorated liver damage induced by I/R.ConclusionControlled low CVP can attenuate hepatic I/R injury in rabbits.
Key words:
Hypotension,controlled; Reperfusioninjury; Liver
Publication Year: 2010
Publication Date: 2010-02-20
Language: en
Type: article
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