Title: Role of mitochondriai ATP-sensitive potassium channels in attenuation of renal ischemia-reperfusion injury by lidocaine pretreatment in rats
Abstract: Objective To evaluate the role of mitochondrial ATP-sensitive potassium (mito-KATP ) chan- nels in attenuation of renal ischemia-reperfusion (I/R) injury by lidocaine pretreatment in rats. Methods Sixty healthy male Wistar rats, weighing 300-350 g, were randomly assigned into 5 groups ( n = 12 each) using a ran- dom number table: sham operation group (group S); renal I/R group (group I/R); lidoeaine pretreatment group (group L) ; 5-HD (a specific blocker of the mito-KATP channel) group and 5-HD + lidocaine pretreatment group (group 5-HD + L). Renal ischemia was induced by occlusion of bilateral renal arteries for 60 rain with atraumatie microelips followed by 4 h reperfusion. At 60 min before renal ischemia, lidocaine 5 mg/kg was intravenously in- jected followed by continuous infusion at 2 mg. kg- 1 . h- 1 in group L. 5-HD 10 mg/kg was injected intraperitoneally at 65 min before ischemia in group 5-HD. In 5-HD + L groups, 5-HD 10 mg/kg was injected intraperitoneally at 65 rain before ischemia and the other procedures were similar to those previously described in group L. In S and I/R groups, the animals received equal volumes of normal saline instead of lidoeaine. Blood samples were obtainedat 6 h of reperfusion for determination of serum creatinine (Cr) and urea mitrogen (BUN) concentrations. Bilateral kidneys were removed for determination of mitochondrial membrane potential in the renal tubular epidural cells, malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity and for microscopic examination. Re- suits Compared with group S, the serum Cr and BUN concentrations and MDA content were significantly in- creased, and SOD activity and mitochondrial membrane potential were decreased in I/R, L, 5-HD and 5-HD + L groups ( P 〈 0.05). Compared with group I/R, the serum Cr and BUN concentrations and MDA content were sig- nificantly decreased, and SOD activity and mitochondrial membrane potential were increased in L and 5-HD + L groups (P 〈 0.05), and no significant changes were found in the serum Cr and BUN concentrations, MDA cont- ent, SOD activity and mitochondrial membrane potential in group 5-HD ( P 〉 0.05). Compared with group L, the serum Cr and BUN concentrations and MDA content were significantly increased, and SOD activity and mitoehon- drial membrane potential were decreased in 5-HD + L group ( P 〈 0.05). The pathological changes were signifi- cantly reduced in group L as compared with I/R and 5-HD + L groups. Conclusion Mito-KATP channels are in- volved in reduction of I/R-induced renal injury by lidocaine pretreatment in rats.
Key words:
KATP channels ; Lidocaine; Reperfusion injury ; Kidney
Publication Year: 2013
Publication Date: 2013-11-20
Language: en
Type: article
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