Title: Isolation and identification of human brucellosis pathogens in Ulanqab of Inner Mongolia
Abstract:Objectve To isolate and identify human brucellosis pathogens in Ulanqab,and to provide a reference for prevention and control of brucellosis.Methods Blood samples of patients with brucellosis were col...Objectve To isolate and identify human brucellosis pathogens in Ulanqab,and to provide a reference for prevention and control of brucellosis.Methods Blood samples of patients with brucellosis were collected; Brucella was isolated by two phase culture bottles.Three standard Brucella strains (Brucella aborttus 544A,Brucella melitensis 16M and Brucella suis 1330S) were used as positive controls and Escherichia coli O:157 was used as a negative control,and 4 Brucella strains were tested.Traditional Brucella identification method,genus specific Brucella surface protein 31 PCR(BCSP31-PCR) and multiplex fluorescence quantitative PCR were applied for isotype identification.Results Four Brucella strains were isolated,which were identified as Brucella via traditional identification method; both BCSP31-PCR amplification and multiplex quantitative PCR were positive,including 3 strains of melitensis 3 type and 1 type strain of melitensis 1 type were identified.Conclusion Brucellosis bacteria have been isolated,which has confirmed Brucella melitensis is the main source of infection for human brucellosis.
Key words:
Brucella; Isolation and purification; Biological assayRead More
Publication Year: 2014
Publication Date: 2014-01-20
Language: en
Type: article
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