Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2135687719', 'doi': 'https://doi.org/10.1016/s1995-7645(13)60073-5', 'title': 'A duplex PCR for rapid and simultaneous detection of Brucella spp. in human blood samples.', 'display_name': 'A duplex PCR for rapid and simultaneous detection of Brucella spp. in human blood samples.', 'publication_year': 2013, 'publication_date': '2013-05-24', 'ids': {'openalex': 'https://openalex.org/W2135687719', 'doi': 'https://doi.org/10.1016/s1995-7645(13)60073-5', 'mag': '2135687719', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/23711705'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1016/s1995-7645(13)60073-5', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S203437272', 'display_name': 'Asian Pacific Journal of Tropical Medicine', 'issn_l': '1995-7645', 'issn': ['1995-7645'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://doi.org/10.1016/s1995-7645(13)60073-5', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5008611710', 'display_name': 'Reza Mirnejad', 'orcid': 'https://orcid.org/0000-0002-7297-3085'}, 'institutions': [{'id': 'https://openalex.org/I120514687', 'display_name': 'Baqiyatallah University of Medical Sciences', 'ror': 'https://ror.org/01ysgtb61', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I120514687']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Reza Mirnejad', 'raw_affiliation_strings': ['Molecular Biology Research Center, #R##N#Baqiyatallah University of Medical Sciences, #R##N#Tehran, Iran'], 'affiliations': [{'raw_affiliation_string': 'Molecular Biology Research Center, #R##N#Baqiyatallah University of Medical Sciences, #R##N#Tehran, Iran', 'institution_ids': ['https://openalex.org/I120514687']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5035613003', 'display_name': 'Mozafar mohamadi', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I120514687', 'display_name': 'Baqiyatallah University of Medical Sciences', 'ror': 'https://ror.org/01ysgtb61', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I120514687']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Mozafar mohamadi', 'raw_affiliation_strings': ['Molecular Biology Research Center, #R##N#Baqiyatallah University of Medical Sciences, #R##N#Tehran, Iran'], 'affiliations': [{'raw_affiliation_string': 'Molecular Biology Research Center, #R##N#Baqiyatallah University of Medical Sciences, #R##N#Tehran, Iran', 'institution_ids': ['https://openalex.org/I120514687']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5042218948', 'display_name': 'Vahhab Piranfar', 'orcid': 'https://orcid.org/0000-0003-3653-5739'}, 'institutions': [{'id': 'https://openalex.org/I120514687', 'display_name': 'Baqiyatallah University of Medical Sciences', 'ror': 'https://ror.org/01ysgtb61', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I120514687']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Vahbeh Piranfar', 'raw_affiliation_strings': ['Molecular Biology Research Center, #R##N#Baqiyatallah University of Medical Sciences, #R##N#Tehran, Iran'], 'affiliations': [{'raw_affiliation_string': 'Molecular Biology Research Center, #R##N#Baqiyatallah University of Medical Sciences, #R##N#Tehran, Iran', 'institution_ids': ['https://openalex.org/I120514687']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5036189323', 'display_name': 'Seied Mojtaba Mortazavi', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I235686326', 'display_name': 'Kerman University of Medical Sciences', 'ror': 'https://ror.org/02kxbqc24', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I235686326']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Seied Mojtaba Mortazavi', 'raw_affiliation_strings': ['Health Station, Kerman University of Medical Sciences, Kerman, Iran'], 'affiliations': [{'raw_affiliation_string': 'Health Station, Kerman University of Medical Sciences, Kerman, Iran', 'institution_ids': ['https://openalex.org/I235686326']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5018851138', 'display_name': 'Reza Kachuei', 'orcid': 'https://orcid.org/0000-0003-3812-5354'}, 'institutions': [{'id': 'https://openalex.org/I120514687', 'display_name': 'Baqiyatallah University of Medical Sciences', 'ror': 'https://ror.org/01ysgtb61', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I120514687']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Reza Kachuei', 'raw_affiliation_strings': ['Molecular Biology Research Center, #R##N#Baqiyatallah University of Medical Sciences, #R##N#Tehran, Iran'], 'affiliations': [{'raw_affiliation_string': 'Molecular Biology Research Center, #R##N#Baqiyatallah University of Medical Sciences, #R##N#Tehran, Iran', 'institution_ids': ['https://openalex.org/I120514687']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 2.498, 'has_fulltext': False, 'cited_by_count': 25, 'citation_normalized_percentile': {'value': 0.834112, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 91, 'max': 92}, 'biblio': {'volume': '6', 'issue': '6', 'first_page': '453', 'last_page': '456'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11899', 'display_name': 'Brucella: diagnosis, epidemiology, treatment', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/3404', 'display_name': 'Small Animals'}, 'field': {'id': 'https://openalex.org/fields/34', 'display_name': 'Veterinary'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11899', 'display_name': 'Brucella: diagnosis, epidemiology, treatment', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/3404', 'display_name': 'Small Animals'}, 'field': {'id': 'https://openalex.org/fields/34', 'display_name': 'Veterinary'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T12951', 'display_name': 'Burkholderia infections and melioidosis', 'score': 0.958, 'subfield': {'id': 'https://openalex.org/subfields/2713', 'display_name': 'Epidemiology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T10486', 'display_name': 'Salmonella and Campylobacter epidemiology', 'score': 0.9379, 'subfield': {'id': 'https://openalex.org/subfields/1106', 'display_name': 'Food Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/brucellaceae', 'display_name': 'Brucellaceae', 'score': 0.53528917}, {'id': 'https://openalex.org/keywords/ovis', 'display_name': 'Ovis', 'score': 0.47917974}, {'id': 'https://openalex.org/keywords/duplex', 'display_name': 'Duplex (building)', 'score': 0.4790755}], 'concepts': [{'id': 'https://openalex.org/C2780767784', 'wikidata': 'https://www.wikidata.org/wiki/Q313358', 'display_name': 'Brucella', 'level': 3, 'score': 0.8621898}, {'id': 'https://openalex.org/C2777692091', 'wikidata': 'https://www.wikidata.org/wiki/Q3512031', 'display_name': 'Brucella melitensis', 'level': 4, 'score': 0.8595097}, {'id': 'https://openalex.org/C2781429187', 'wikidata': 'https://www.wikidata.org/wiki/Q156050', 'display_name': 'Brucellosis', 'level': 2, 'score': 0.79014045}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.55585456}, {'id': 'https://openalex.org/C2781028839', 'wikidata': 'https://www.wikidata.org/wiki/Q3290077', 'display_name': 'Brucellaceae', 'level': 5, 'score': 0.53528917}, {'id': 'https://openalex.org/C2777963300', 'wikidata': 'https://www.wikidata.org/wiki/Q22724', 'display_name': 'Ovis', 'level': 2, 'score': 0.47917974}, {'id': 'https://openalex.org/C99611785', 'wikidata': 'https://www.wikidata.org/wiki/Q6453233', 'display_name': 'Duplex (building)', 'level': 3, 'score': 0.4790755}, {'id': 'https://openalex.org/C49105822', 'wikidata': 'https://www.wikidata.org/wiki/Q176996', 'display_name': 'Polymerase chain reaction', 'level': 3, 'score': 0.46559393}, {'id': 'https://openalex.org/C89423630', 'wikidata': 'https://www.wikidata.org/wiki/Q7193', 'display_name': 'Microbiology', 'level': 1, 'score': 0.4609611}, {'id': 'https://openalex.org/C159047783', 'wikidata': 'https://www.wikidata.org/wiki/Q7215', 'display_name': 'Virology', 'level': 1, 'score': 0.29562932}, {'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.26390904}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.14907825}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.057361394}, {'id': 'https://openalex.org/C18903297', 'wikidata': 'https://www.wikidata.org/wiki/Q7150', 'display_name': 'Ecology', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D016470', 'descriptor_name': 'Bacteremia', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': True}, {'descriptor_ui': 'D002002', 'descriptor_name': 'Brucella', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D002006', 'descriptor_name': 'Brucellosis', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': True}, {'descriptor_ui': 'D016133', 'descriptor_name': 'Polymerase Chain Reaction', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D000328', 'descriptor_name': 'Adult', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016470', 'descriptor_name': 'Bacteremia', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016470', 'descriptor_name': 'Bacteremia', 'qualifier_ui': 'Q000175', 'qualifier_name': 'diagnosis', 'is_major_topic': False}, {'descriptor_ui': 'D002002', 'descriptor_name': 'Brucella', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002002', 'descriptor_name': 'Brucella', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D002006', 'descriptor_name': 'Brucellosis', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002006', 'descriptor_name': 'Brucellosis', 'qualifier_ui': 'Q000097', 'qualifier_name': 'blood', 'is_major_topic': False}, {'descriptor_ui': 'D002006', 'descriptor_name': 'Brucellosis', 'qualifier_ui': 'Q000175', 'qualifier_name': 'diagnosis', 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': 'Q000097', 'qualifier_name': 'blood', 'is_major_topic': False}, {'descriptor_ui': 'D004587', 'descriptor_name': 'Electrophoresis, Agar Gel', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005260', 'descriptor_name': 'Female', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008297', 'descriptor_name': 'Male', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016133', 'descriptor_name': 'Polymerase Chain Reaction', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1016/s1995-7645(13)60073-5', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S203437272', 'display_name': 'Asian Pacific Journal of Tropical Medicine', 'issn_l': '1995-7645', 'issn': ['1995-7645'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/23711705', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1016/s1995-7645(13)60073-5', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S203437272', 'display_name': 'Asian Pacific Journal of Tropical Medicine', 'issn_l': '1995-7645', 'issn': ['1995-7645'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'display_name': 'Life on land', 'score': 0.47, 'id': 'https://metadata.un.org/sdg/15'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 24, 'referenced_works': ['https://openalex.org/W135800887', 'https://openalex.org/W1545635279', 'https://openalex.org/W1559720266', 'https://openalex.org/W1997389065', 'https://openalex.org/W2004209899', 'https://openalex.org/W2004850102', 'https://openalex.org/W2016525828', 'https://openalex.org/W2039099129', 'https://openalex.org/W2065362201', 'https://openalex.org/W2069029552', 'https://openalex.org/W2070561755', 'https://openalex.org/W2076496180', 'https://openalex.org/W2098920656', 'https://openalex.org/W2099027220', 'https://openalex.org/W2101131276', 'https://openalex.org/W2103501562', 'https://openalex.org/W2127824744', 'https://openalex.org/W2128988380', 'https://openalex.org/W2184170347', 'https://openalex.org/W2187774365', 'https://openalex.org/W2411511565', 'https://openalex.org/W40409475', 'https://openalex.org/W4211033510', 'https://openalex.org/W4285719527'], 'related_works': ['https://openalex.org/W3028893531', 'https://openalex.org/W2600852902', 'https://openalex.org/W2494279337', 'https://openalex.org/W2379026548', 'https://openalex.org/W2372950123', 'https://openalex.org/W2169087453', 'https://openalex.org/W2162386698', 'https://openalex.org/W2141560699', 'https://openalex.org/W2105113619', 'https://openalex.org/W1967817769'], 'abstract_inverted_index': {'To': [0], 'design': [1], 'a': [2, 117], 'duplex': [3, 111], 'PCR': [4, 31, 112], 'for': [5, 73, 82, 92, 124], 'rapid': [6], 'and': [7, 42, 52, 90, 120], 'simultaneous': [8, 125], 'detection': [9, 126], 'of': [10, 47, 127, 130], 'Brucella': [11, 49, 74, 83, 93, 131], 'species.': [12], 'in': [13, 50, 67, 104, 132], 'human': [14], 'blood': [15], 'samples.': [16, 134], 'Fifty-two': [17], 'peripheral': [18, 57], 'bloods': [19, 58], 'samples': [20, 59, 70], 'were': [21, 33, 71, 109], 'collected': [22], 'from': [23], 'suspicious': [24], 'patients': [25], 'with': [26], 'brucellosis.': [27], 'Following': [28], 'DNA': [29], 'extraction,': [30], 'assay': [32], 'performed,': [34], 'using': [35], 'three': [36, 44], 'primers': [37, 108], 'that': [38, 103], 'could': [39], 'simultaneously': [40], 'identify': [41], 'differentiate': [43], 'major': [45], 'species': [46, 129], 'pathogenic': [48], 'humans': [51], 'animals.': [53], 'Of': [54], 'the': [55], '52': [56], 'tested,': [60], '25': [61], 'sample': [62], '(48%)': [63], 'showed': [64], 'positive': [65, 72], 'reactions': [66], 'PCR.': [68], 'Twelve': [69], 'abortus': [75, 78], '39': [76, 85, 95], '(B.': [77, 86, 96], '39)': [79, 88, 98], '(23%),': [80], '13': [81], 'melitensis': [84, 87], '(25%)': [89], '0': [91], 'ovis': [94, 97], '(0%).': [99], 'This': [100], 'work': [101], 'de=monstrates': [102], 'case': [105], 'where': [106], 'specific': [107], 'utilized,': [110], 'has': [113], 'proved': [114], 'to': [115], 'be': [116], 'simple,': [118], 'fast,': [119], 'relatively': [121], 'inexpensive': [122], 'method': [123], 'important': [128], 'clinical': [133]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2135687719', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 2}, {'year': 2020, 'cited_by_count': 2}, {'year': 2019, 'cited_by_count': 4}, {'year': 2018, 'cited_by_count': 3}, {'year': 2017, 'cited_by_count': 6}, {'year': 2016, 'cited_by_count': 4}, {'year': 2015, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 2}], 'updated_date': '2024-12-12T09:05:15.396391', 'created_date': '2016-06-24'}