Title: Effect of carbon monoxide on lipid peroxidation and activities of Na+-K+ ATPase in focal ischemic brain tissue and the neuronal expression of heme oxygenase protein in rats
Abstract: Objective To study the effect of carbon monoxide (CO) on lipid peroxidation and activities of Na+-K+ ATPase of local cerebral ischemia and the expression of heme oxygenase protein dur-ing early focal ischemia in rats. Methods SD rats were divided into three groups randomly,including he-min group,ZnPP group and saline group. Twelve h before,and 24 h after infarction,the concentrations of CO and MDA, and activities of SOD and Na+-K+ ATPase were examined. The expression of heine oxygen-ase-1(HO-1) protein in the brain of rats during early focal cerebral ischemia was detected by using im-munohistochemistry and computer image analysis in middle cerebral artery occlusion (MCAO) model of rats. Results Compared with the saline group, the concentration of CO in blood was significantly in-creased by 1.36-fold in Heroin group (P<0.01), and that was significantly decreased by 26.4 % in ZnPP group (P<0.01). In Hemin group, MDA was decreased by 11.3% (P<0.01) and the activities of SOD and Na+-K+ ATPase were increased by 6.85% and 20.1% respectively (all P<0.05) as compared with saline group. In ZnPP group, MDA was increased by 12.4% (P<0.05), but the activities of SOD and Na+-K+ ATPase were decreased by 8.2% and 18.9% respectively (all P <0.05) in comparison to saline group. HO-1 expression was detectable in the neurons and gliacytes of the ipsilateral cerebral cortex infarc-tion area at 30 min after MCAO. With time going on, the expression of HO-1 was increased. Conclusion CO can protect brain tissues wfocal cerebral ischemia. The expression of HO-1 protein is evidently sig-nificant physiological consequences in the recovery of neuronal tissue from ischemic injury in focal cerebral ischemia.
Key words:
Focal cerebral ischemia; Carbon monoxide; Lipid peroxidation; Heine oxygen-ase
Publication Year: 2009
Publication Date: 2009-04-08
Language: en
Type: article
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