Title: Effect of blood glucose level on cerebral ischemia-reperfusion injury of rats
Abstract: Objective To discuss the influence of blood glucose on oxidative stress in animal cerebral ischemic-reperfusion models. Methods Acute cerebral ischemia-reperfusion models were made by bilateral carotid artery ligation for30 minutes and then reperfusion for 2 hours. Male Wistar rats were randomly divided into 4 groups: sham operation group(group A), normal saline control group(group B), insulin group(2.1 U/kg, group C), insulin(2.1 U/kg) and 50%glucose(2 g/kg) group(group D). After operation, the brain tissue specimens were sectioned to observe the change of ultrastructure. The content of methane dicarboxylic aldehyde(MDA, product of lipid peroxidation), superoxide dismutase(SOD), and the activity of ATP enzyme were detected. Results 1Blood glucose levels in group A and D were within normal limits(4.6-10 mmol/L), blood glucose in group B increased significantly(P 0.01), blood glucose in group C decreased significantly(P 0.01); 2MDA and SOD content, ATP-ase activity in brain tissue of group B and group C had no significant difference(P 0.05); compared with the group B, MDA was reduced(P 0.05), SOD was elevated(P 0.05), and ATP-ase activity was increased(P 0.05) in group D. Conclusion 1Cerebral ischemiareperfusion can increase blood glucose, and cause oxidative stress in cerebral tissue. 2During ischemia reperfusion, if blood glucose level is too low, can aggravate oxidative stress. 3Control blood glucose to upper limit of normal range can reduce oxidative stress.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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