Title: Effect and Mechanism of Naringenin on the Proliferation of Human Leukemia K562 Cell Line
Abstract:Objective: To investigate the effect and mechanism of naringenin on human leukemia K562 cell line.Methods: K562 cell line cultured in vitro was exposed to different concentration of naringenin and the...Objective: To investigate the effect and mechanism of naringenin on human leukemia K562 cell line.Methods: K562 cell line cultured in vitro was exposed to different concentration of naringenin and the cell proliferation manner was detected by MTT assay.K562 cells morphology and apoptosis were observed under light microscope with Wight-Giemsa staining and Hochest33258 staining and under transmission electron microscope.Flow cytometry was used to determine the cell cycle and apoptosis rate,and cell PCNA protein was detected by immunhistochemical staining.Results: Proliferation of K562 cells was significantly inhibited by naringenin and the IC50 at 24,48 and 72 h were 455,225 and 175 μmol/L respectively.Cell growth-inhibition and apoptosis were observed under light microscope and transmission electron microscope.Observation by flow cytometry indicated that K562 cells were blocked in G0/G1 phase of cell cycle in naringenin group,and S phase cells were remarkably decreased.PCNA expression in each treatment group was remarkably less than in control group.Conclusion: Naringenin has a significant inhibitory effect on the growth of K562 cells in vitro,and the cell cycle arrest and the induction of apoptosis may be involved in it.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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