Title: Inhibitory effects of silibinin on proliferation and apoptosis induction of human bladder cancer T24 and 5637 cell lines
Abstract: Objective To determine the effects of silibinin(SB)on the proliferation and apoptosis of human bladder cancer T24and 5637cell lines,and to clarify the possible mechanism.Methods Human bladder cancer 5637and T24cell lines were cultured in cell media and the 5637and T24cells at logarithmic growth phase were divided into control group(0μmol·L-1 SB)and 25,50,100,200,and 400μmol·L-1 SB groups.The inhibitory effects of SB on the proliferation of T24and 5637cells were detected by MTT assay;inverted phase contrast microscope was used to observe the invasion inhibition ability of T24and 5637cell lines after treated with different contentations of SB;the morphological changes of T24and 5637cells after treatment were observed by DAPI staining;the apoptosis was detected and evaluated by flow cytometry.Results The MTT results showed that the viability rates of T24and 5637cell lines were decreased in different concentrations of SB groups in a time-and concentrationdependent manner compared with control group.After treated with different concentrations of SB,the migration of T24and 5637cell lines was significantly inhibited.The DAPI staining and flow cytometry results revealed that the apoptotic rates of T24and 5637cells in different concentrations of SB groups were higher than those in control group(P0.05).Conclusion SB can inhibit the proliferation and invasion of human bladder cancer T24and 5637cell lines through inducing the apoptosis.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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Cited By Count: 1
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