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Title: $Modification of Cre gene by PCR method.
Abstract: Cre/LoxP site-specified recombination system is mainly used for excision,inversion and integration of target gene.Therefore,this system can be used for plant marker free genetic transformation,site-specific transgene expression and so on.However,the application of this system was limited due to its low expression and excision efficiency.In this study,an intron,which can enhance gene expression in plants,was inserted into Cre by using PCR method.And a modified Cre gene,named Crein,was obtained.This gene was composed of three parts: ATG,intron,Cre gene without ATG.This gene can be used for further research to provide the good system for plant marker free transformation.