Title: Genotypical differences in callus induction and regeneration of plantlets produced from asparagus (Asparagus officinalis L.) anther cultures
Abstract: Summary It was the aim of this study to determine genotypical differences for callus induction and differentiation of asparagus (Asparagus officinalis L.) anther cultures. At the uninucleate stage, anthers of three asparagus cultivars, „Huchels Alpha“, „Record“ and „Schwetzinger Meisterschuss“, were cultured on Murashige and Skoog medium (MS) supplemented with 3% sucrose, 0.1 mg L -1 naphthalene acetic acid (NAA), 0.5 mg L -1 benzyladenine (BA) and 0.5 mg L -1 2,4-Dichloro-phenoxyacetic acid (2,4-D). The percentage of responding anthers ranged from 40% to 66%. The cultivar „Record“ showed the highest percentage of responding anthers, „Huchels Alpha“ the lowest. The calli were transferred to the following media for differentiation: MS medium supplemented with 3% sucrose, 0.3 mg L -1 BA and 0.2 mg L -1 NAA (medium A) and MS medium supplemented with 3% sucrose, 0.1 mg L -1 NAA, 0.1mg L -1 kinetin and 0.65 mg L -1 ancymidol (medium B). The cultivar 'Record' produced the highest number of embryogenic calli on medium A. This cultivar showed in both growth media the highest number of embryos per dish, embryos per callus, plantlets per dish and number of plantlets per callus.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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