Title: Study of Differential Gene Expression Profiling in Rat Peripheral Blood Lymphocyte 12 Hours after in-vitro and Whole-body Exposure to 2Gy Gamma Ray
Abstract: Objective To evaluate the changes of differential gene expression in peripheral blood lymphocyte of SD rats 12 hours after in-vitro and whole-body exposure to 2Gy 60Co γ-ray,and provide the evidence for irradiation damage in gene level.Methods The method of microarray was applied to detect the differentially expressed genes and analyze the pathways.Gene expression was measured using real-time quantitative PCR to validate the results of the microarray.Results The results showed that there were 2 001 genes differentially expressed over 3 fold in in-vitro group,2 590 genes in whole-body group,and 312 genes in both in-vitro and whole-body groups differentially expressed.There were 22 KEGG pathways in in-vitro group,35 KEGG pathways in whole-body group,and 10 KEGG pathways in both.The relative quantity’s results of three genes,selected to validate the microarray,were consistent with microarray results.Conclusion These results indicate that the differentially expressed genes are related to apoptosis,cell cycle and signal transduction etc,which will provide a basis for the further study of mechanism of irradiation damage.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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