Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2955651119', 'doi': 'https://doi.org/10.1007/s12010-019-03059-1', 'title': 'An Efficient Approach for Two Distal Point Site-Directed Mutagenesis from Randomly Ligated PCR Products', 'display_name': 'An Efficient Approach for Two Distal Point Site-Directed Mutagenesis from Randomly Ligated PCR Products', 'publication_year': 2019, 'publication_date': '2019-07-01', 'ids': {'openalex': 'https://openalex.org/W2955651119', 'doi': 'https://doi.org/10.1007/s12010-019-03059-1', 'mag': '2955651119', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/31264104'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1007/s12010-019-03059-1', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S153285774', 'display_name': 'Applied Biochemistry and Biotechnology', 'issn_l': '0273-2289', 'issn': ['0273-2289', '1559-0291'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319900', 'host_organization_name': 'Springer Science+Business Media', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310319900'], 'host_organization_lineage_names': ['Springer Nature', 'Springer Science+Business Media'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5062569530', 'display_name': 'Jafar Khezri', 'orcid': 'https://orcid.org/0000-0003-4418-2742'}, 'institutions': [{'id': 'https://openalex.org/I180634299', 'display_name': 'University of Sistan and Baluchestan', 'ror': 'https://ror.org/02n43xw86', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I180634299']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Jafar Khezri', 'raw_affiliation_strings': ['Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran'], 'affiliations': [{'raw_affiliation_string': 'Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran', 'institution_ids': ['https://openalex.org/I180634299']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5030214177', 'display_name': 'Bagher Yakhchali', 'orcid': 'https://orcid.org/0000-0003-4031-877X'}, 'institutions': [{'id': 'https://openalex.org/I4210137727', 'display_name': 'National Institute of Genetic Engineering and Biotechnology', 'ror': 'https://ror.org/03ckh6215', 'country_code': 'IR', 'type': 'facility', 'lineage': ['https://openalex.org/I4210137727']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Bagher Yakhchali', 'raw_affiliation_strings': ['Department of Industrial and Environmental Biotechnology, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran'], 'affiliations': [{'raw_affiliation_string': 'Department of Industrial and Environmental Biotechnology, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran', 'institution_ids': ['https://openalex.org/I4210137727']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5021096366', 'display_name': 'Ali Asghar Karkhane', 'orcid': 'https://orcid.org/0000-0002-3521-0409'}, 'institutions': [{'id': 'https://openalex.org/I4210137727', 'display_name': 'National Institute of Genetic Engineering and Biotechnology', 'ror': 'https://ror.org/03ckh6215', 'country_code': 'IR', 'type': 'facility', 'lineage': ['https://openalex.org/I4210137727']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Ali Asghar Karkhane', 'raw_affiliation_strings': ['Department of Industrial and Environmental Biotechnology, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran'], 'affiliations': [{'raw_affiliation_string': 'Department of Industrial and Environmental Biotechnology, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran', 'institution_ids': ['https://openalex.org/I4210137727']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5031177863', 'display_name': 'Mohammad Hossein Sangtarash', 'orcid': 'https://orcid.org/0000-0002-1314-3225'}, 'institutions': [{'id': 'https://openalex.org/I180634299', 'display_name': 'University of Sistan and Baluchestan', 'ror': 'https://ror.org/02n43xw86', 'country_code': 'IR', 'type': 'education', 'lineage': ['https://openalex.org/I180634299']}], 'countries': ['IR'], 'is_corresponding': False, 'raw_author_name': 'Mohammad Hossein Sangtarash', 'raw_affiliation_strings': ['Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran'], 'affiliations': [{'raw_affiliation_string': 'Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran', 'institution_ids': ['https://openalex.org/I180634299']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2790, 'currency': 'EUR', 'value_usd': 3590, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 0.0, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 0, 'citation_normalized_percentile': {'value': 0.0, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 0, 'max': 61}, 'biblio': {'volume': '189', 'issue': '4', 'first_page': '1318', 'last_page': '1326'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11970', 'display_name': 'Molecular Biology Techniques and Applications', 'score': 0.9994, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11970', 'display_name': 'Molecular Biology Techniques and Applications', 'score': 0.9994, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10878', 'display_name': 'CRISPR and Genetic Engineering', 'score': 0.9983, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11048', 'display_name': 'Bacteriophages and microbial interactions', 'score': 0.9958, 'subfield': {'id': 'https://openalex.org/subfields/2303', 'display_name': 'Ecology'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/site-directed-mutagenesis', 'display_name': 'Site-directed mutagenesis', 'score': 0.6581757}, {'id': 'https://openalex.org/keywords/restriction-site', 'display_name': 'Restriction site', 'score': 0.6541853}, {'id': 'https://openalex.org/keywords/restriction-digest', 'display_name': 'Restriction digest', 'score': 0.59014434}, {'id': 'https://openalex.org/keywords/primer-dimer', 'display_name': 'Primer dimer', 'score': 0.48188478}], 'concepts': [{'id': 'https://openalex.org/C16318435', 'wikidata': 'https://www.wikidata.org/wiki/Q149299', 'display_name': 'Mutagenesis', 'level': 4, 'score': 0.7852529}, {'id': 'https://openalex.org/C103408237', 'wikidata': 'https://www.wikidata.org/wiki/Q2642668', 'display_name': 'Site-directed mutagenesis', 'level': 4, 'score': 0.6581757}, {'id': 'https://openalex.org/C58301748', 'wikidata': 'https://www.wikidata.org/wiki/Q1074969', 'display_name': 'Restriction site', 'level': 4, 'score': 0.6541853}, {'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.6128689}, {'id': 'https://openalex.org/C2889275', 'wikidata': 'https://www.wikidata.org/wiki/Q7316313', 'display_name': 'Restriction digest', 'level': 4, 'score': 0.59014434}, {'id': 'https://openalex.org/C195116880', 'wikidata': 'https://www.wikidata.org/wiki/Q4859565', 'display_name': 'Sticky and blunt ends', 'level': 4, 'score': 0.5614153}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.52065843}, {'id': 'https://openalex.org/C176944494', 'wikidata': 'https://www.wikidata.org/wiki/Q1415380', 'display_name': 'Point mutation', 'level': 4, 'score': 0.50074196}, {'id': 'https://openalex.org/C20682886', 'wikidata': 'https://www.wikidata.org/wiki/Q475229', 'display_name': 'Inverse polymerase chain reaction', 'level': 5, 'score': 0.49654227}, {'id': 'https://openalex.org/C22744801', 'wikidata': 'https://www.wikidata.org/wiki/Q172778', 'display_name': 'Plasmid', 'level': 3, 'score': 0.49323958}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.4911618}, {'id': 'https://openalex.org/C52001828', 'wikidata': 'https://www.wikidata.org/wiki/Q2902493', 'display_name': 'Primer dimer', 'level': 5, 'score': 0.48188478}, {'id': 'https://openalex.org/C128319531', 'wikidata': 'https://www.wikidata.org/wiki/Q219715', 'display_name': 'Restriction enzyme', 'level': 3, 'score': 0.4799357}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.46125543}, {'id': 'https://openalex.org/C49105822', 'wikidata': 'https://www.wikidata.org/wiki/Q176996', 'display_name': 'Polymerase chain reaction', 'level': 3, 'score': 0.40099514}, {'id': 'https://openalex.org/C143065580', 'wikidata': 'https://www.wikidata.org/wiki/Q3285695', 'display_name': 'Mutant', 'level': 3, 'score': 0.38396454}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.29289645}, {'id': 'https://openalex.org/C157431215', 'wikidata': 'https://www.wikidata.org/wiki/Q856465', 'display_name': 'Nested polymerase chain reaction', 'level': 4, 'score': 0.11896148}, {'id': 'https://openalex.org/C90583042', 'wikidata': 'https://www.wikidata.org/wiki/Q1952456', 'display_name': 'Multiplex polymerase chain reaction', 'level': 4, 'score': 0.07302678}], 'mesh': [{'descriptor_ui': 'D003001', 'descriptor_name': 'Cloning, Molecular', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D017931', 'descriptor_name': 'DNA Primers', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D016297', 'descriptor_name': 'Mutagenesis, Site-Directed', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D016133', 'descriptor_name': 'Polymerase Chain Reaction', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D017931', 'descriptor_name': 'DNA Primers', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017931', 'descriptor_name': 'DNA Primers', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D005822', 'descriptor_name': 'Genetic Vectors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005822', 'descriptor_name': 'Genetic Vectors', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D005822', 'descriptor_name': 'Genetic Vectors', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D016297', 'descriptor_name': 'Mutagenesis, Site-Directed', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1007/s12010-019-03059-1', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S153285774', 'display_name': 'Applied Biochemistry and Biotechnology', 'issn_l': '0273-2289', 'issn': ['0273-2289', '1559-0291'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319900', 'host_organization_name': 'Springer Science+Business Media', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310319900'], 'host_organization_lineage_names': ['Springer Nature', 'Springer Science+Business Media'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/31264104', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 29, 'referenced_works': ['https://openalex.org/W122556579', 'https://openalex.org/W136701882', 'https://openalex.org/W1627391527', 'https://openalex.org/W1964109131', 'https://openalex.org/W1982149924', 'https://openalex.org/W1985346839', 'https://openalex.org/W2013090583', 'https://openalex.org/W2024415730', 'https://openalex.org/W2034561173', 'https://openalex.org/W2037900142', 'https://openalex.org/W2039203958', 'https://openalex.org/W2048102351', 'https://openalex.org/W2050558966', 'https://openalex.org/W2056679161', 'https://openalex.org/W2060913542', 'https://openalex.org/W2075215734', 'https://openalex.org/W2075566523', 'https://openalex.org/W2077892824', 'https://openalex.org/W2106494856', 'https://openalex.org/W2145983694', 'https://openalex.org/W2165151174', 'https://openalex.org/W2173668937', 'https://openalex.org/W2197537607', 'https://openalex.org/W22840390', 'https://openalex.org/W2412054606', 'https://openalex.org/W2506112079', 'https://openalex.org/W2516007520', 'https://openalex.org/W2766368217', 'https://openalex.org/W2922288262'], 'related_works': ['https://openalex.org/W787269129', 'https://openalex.org/W2955651119', 'https://openalex.org/W2464720041', 'https://openalex.org/W2146845516', 'https://openalex.org/W209814502', 'https://openalex.org/W2087434084', 'https://openalex.org/W2077097444', 'https://openalex.org/W2030043662', 'https://openalex.org/W2028702152', 'https://openalex.org/W11602674'], 'abstract_inverted_index': None, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2955651119', 'counts_by_year': [], 'updated_date': '2024-12-07T22:35:01.452430', 'created_date': '2019-07-12'}