Title: Cloning and identification of the {\sl rag}1 gene in channel catfish
Abstract: Three fragments of rag1 gene were obtained with a total length of 3218 bp. An ORF of 2235 bp and an intron of 293 bp within the sequence were identified. The percentage identity of the sequence with rag1 gene of other species was more than 70%. The multiple alignment of the cDNA sequence with rag1 complete cDNA sequences in zebrafish, rainbow trout, and bull shark indicted that the proportion of identical base sites was 43.9%. The proportion of identical base sites from the 1st to the 1351 st was 33.2%, and the proportion of identical base sites from the 1352nd to th 2925th was 53.1%. There was a significant diversity between these two regions (P<0.01).
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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