Title: Cloning and characterization of a gene coding reverse transcriptase from Nosema bombycis
Abstract: Partial cDNA sequence about 1.2kb coding reverse transcriptase gene from Nosema bombycis was amplified by RT-PCR. After cloning and sequencing, the result showed that there were not original codon and stop codon. So gene specific primers were designed based on the sequence to amplify its 5^-terminal and 3^-terminal sequences. After that, the products of 3^-RACE showed that there were stop codon (TGA) and polyA in the 3^-end. But the original codon (ATG) hasn^t been found after 5^-RACE for twice. Ultimately, we got cDNA sequence for 3 303bp and predicted a peptide of 1 101 amino acids, but not full length. And then we used BLAST to consult GenBank database and knew it was high homologous with reverse transcriptase genes of many organisms.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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