Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2883076366', 'doi': 'https://doi.org/10.1186/s12896-018-0456-8', 'title': 'pXST, a novel vector for TA cloning and blunt-end cloning', 'display_name': 'pXST, a novel vector for TA cloning and blunt-end cloning', 'publication_year': 2018, 'publication_date': '2018-07-13', 'ids': {'openalex': 'https://openalex.org/W2883076366', 'doi': 'https://doi.org/10.1186/s12896-018-0456-8', 'mag': '2883076366', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/30005664', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/6045858'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1186/s12896-018-0456-8', 'pdf_url': 'https://bmcbiotechnol.biomedcentral.com/track/pdf/10.1186/s12896-018-0456-8', 'source': {'id': 'https://openalex.org/S53489610', 'display_name': 'BMC Biotechnology', 'issn_l': '1472-6750', 'issn': ['1472-6750'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320256', 'host_organization_name': 'BioMed Central', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310320256'], 'host_organization_lineage_names': ['Springer Nature', 'BioMed Central'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'doaj', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://bmcbiotechnol.biomedcentral.com/track/pdf/10.1186/s12896-018-0456-8', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5074097861', 'display_name': 'Qin Liu', 'orcid': 'https://orcid.org/0000-0002-2549-3425'}, 'institutions': [{'id': 'https://openalex.org/I20942203', 'display_name': 'Hainan University', 'ror': 'https://ror.org/03q648j11', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I20942203']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Qin Liu', 'raw_affiliation_strings': ['Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China'], 'affiliations': [{'raw_affiliation_string': 'Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China', 'institution_ids': ['https://openalex.org/I20942203']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5035701136', 'display_name': 'Dang HuiJie', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I20942203', 'display_name': 'Hainan University', 'ror': 'https://ror.org/03q648j11', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I20942203']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Hui-Jie Dang', 'raw_affiliation_strings': ['Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China'], 'affiliations': [{'raw_affiliation_string': 'Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China', 'institution_ids': ['https://openalex.org/I20942203']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5052184277', 'display_name': 'Yuanhang Wu', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I20942203', 'display_name': 'Hainan University', 'ror': 'https://ror.org/03q648j11', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I20942203']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Yuan-Hang Wu', 'raw_affiliation_strings': ['Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China'], 'affiliations': [{'raw_affiliation_string': 'Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China', 'institution_ids': ['https://openalex.org/I20942203']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5100643214', 'display_name': 'Li M', 'orcid': 'https://orcid.org/0000-0003-1350-9909'}, 'institutions': [{'id': 'https://openalex.org/I20942203', 'display_name': 'Hainan University', 'ror': 'https://ror.org/03q648j11', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I20942203']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Min Li', 'raw_affiliation_strings': ['Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China'], 'affiliations': [{'raw_affiliation_string': 'Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China', 'institution_ids': ['https://openalex.org/I20942203']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5028658037', 'display_name': 'Yinhua Chen', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I20942203', 'display_name': 'Hainan University', 'ror': 'https://ror.org/03q648j11', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I20942203']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Yin-Hua Chen', 'raw_affiliation_strings': ['Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China'], 'affiliations': [{'raw_affiliation_string': 'Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China', 'institution_ids': ['https://openalex.org/I20942203']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5101760086', 'display_name': 'Xiaolei Niu', 'orcid': 'https://orcid.org/0000-0001-8857-6044'}, 'institutions': [{'id': 'https://openalex.org/I20942203', 'display_name': 'Hainan University', 'ror': 'https://ror.org/03q648j11', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I20942203']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Xiao-Lei Niu', 'raw_affiliation_strings': ['Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China'], 'affiliations': [{'raw_affiliation_string': 'Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China', 'institution_ids': ['https://openalex.org/I20942203']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5075448396', 'display_name': 'Kaimian Li', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I107851509', 'display_name': 'Chinese Academy of Tropical Agricultural Sciences', 'ror': 'https://ror.org/003qeh975', 'country_code': 'CN', 'type': 'government', 'lineage': ['https://openalex.org/I107851509', 'https://openalex.org/I4210127390', 'https://openalex.org/I4210151987']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Kai-Mian Li', 'raw_affiliation_strings': ["Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 570228, People's Republic of China"], 'affiliations': [{'raw_affiliation_string': "Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 570228, People's Republic of China", 'institution_ids': ['https://openalex.org/I107851509']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5036092497', 'display_name': 'Lijuan Luo', 'orcid': 'https://orcid.org/0000-0002-4397-6375'}, 'institutions': [{'id': 'https://openalex.org/I20942203', 'display_name': 'Hainan University', 'ror': 'https://ror.org/03q648j11', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I20942203']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Li-Juan Luo', 'raw_affiliation_strings': ['Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China'], 'affiliations': [{'raw_affiliation_string': 'Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China', 'institution_ids': ['https://openalex.org/I20942203']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 1570, 'currency': 'GBP', 'value_usd': 1925, 'provenance': 'doaj'}, 'apc_paid': {'value': 1570, 'currency': 'GBP', 'value_usd': 1925, 'provenance': 'doaj'}, 'fwci': 0.438, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 6, 'citation_normalized_percentile': {'value': 0.580121, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 80, 'max': 82}, 'biblio': {'volume': '18', 'issue': '1', 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10878', 'display_name': 'CRISPR and Genetic Engineering', 'score': 0.9995, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10878', 'display_name': 'CRISPR and Genetic Engineering', 'score': 0.9995, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12929', 'display_name': 'Animal Genetics and Reproduction', 'score': 0.998, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10725', 'display_name': 'RNA Interference and Gene Delivery', 'score': 0.9958, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/cloning', 'display_name': 'Cloning (programming)', 'score': 0.78718925}, {'id': 'https://openalex.org/keywords/cloning-vector', 'display_name': 'Cloning vector', 'score': 0.6969466}, {'id': 'https://openalex.org/keywords/multiple-cloning-site', 'display_name': 'Multiple cloning site', 'score': 0.6124928}, {'id': 'https://openalex.org/keywords/restriction-digest', 'display_name': 'Restriction digest', 'score': 0.566023}, {'id': 'https://openalex.org/keywords/library', 'display_name': 'Library', 'score': 0.51961225}, {'id': 'https://openalex.org/keywords/restriction-site', 'display_name': 'Restriction site', 'score': 0.46796164}, {'id': 'https://openalex.org/keywords/clone', 'display_name': 'clone (Java method)', 'score': 0.42005262}], 'concepts': [{'id': 'https://openalex.org/C121050878', 'wikidata': 'https://www.wikidata.org/wiki/Q5135020', 'display_name': 'Cloning (programming)', 'level': 2, 'score': 0.78718925}, {'id': 'https://openalex.org/C168374021', 'wikidata': 'https://www.wikidata.org/wiki/Q1924179', 'display_name': 'Cloning vector', 'level': 5, 'score': 0.6969466}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.6727234}, {'id': 'https://openalex.org/C179437574', 'wikidata': 'https://www.wikidata.org/wiki/Q15089798', 'display_name': 'Ligation', 'level': 2, 'score': 0.6151773}, {'id': 'https://openalex.org/C107618985', 'wikidata': 'https://www.wikidata.org/wiki/Q358963', 'display_name': 'Multiple cloning site', 'level': 5, 'score': 0.6124928}, {'id': 'https://openalex.org/C195116880', 'wikidata': 'https://www.wikidata.org/wiki/Q4859565', 'display_name': 'Sticky and blunt ends', 'level': 4, 'score': 0.58234906}, {'id': 'https://openalex.org/C22744801', 'wikidata': 'https://www.wikidata.org/wiki/Q172778', 'display_name': 'Plasmid', 'level': 3, 'score': 0.56977385}, {'id': 'https://openalex.org/C2889275', 'wikidata': 'https://www.wikidata.org/wiki/Q7316313', 'display_name': 'Restriction digest', 'level': 4, 'score': 0.566023}, {'id': 'https://openalex.org/C19924922', 'wikidata': 'https://www.wikidata.org/wiki/Q408351', 'display_name': 'Molecular cloning', 'level': 4, 'score': 0.5555332}, {'id': 'https://openalex.org/C1436000', 'wikidata': 'https://www.wikidata.org/wiki/Q526826', 'display_name': 'Library', 'level': 4, 'score': 0.51961225}, {'id': 'https://openalex.org/C204241405', 'wikidata': 'https://www.wikidata.org/wiki/Q461499', 'display_name': 'Transformation (genetics)', 'level': 3, 'score': 0.5128041}, {'id': 'https://openalex.org/C92087593', 'wikidata': 'https://www.wikidata.org/wiki/Q427389', 'display_name': 'Vector (molecular biology)', 'level': 4, 'score': 0.4986105}, {'id': 'https://openalex.org/C58301748', 'wikidata': 'https://www.wikidata.org/wiki/Q1074969', 'display_name': 'Restriction site', 'level': 4, 'score': 0.46796164}, {'id': 'https://openalex.org/C70721500', 'wikidata': 'https://www.wikidata.org/wiki/Q177005', 'display_name': 'Computational biology', 'level': 1, 'score': 0.4540168}, {'id': 'https://openalex.org/C128319531', 'wikidata': 'https://www.wikidata.org/wiki/Q219715', 'display_name': 'Restriction enzyme', 'level': 3, 'score': 0.45336324}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.4308869}, {'id': 'https://openalex.org/C81089528', 'wikidata': 'https://www.wikidata.org/wiki/Q5134986', 'display_name': 'clone (Java method)', 'level': 3, 'score': 0.42005262}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.38641316}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.2562443}, {'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.25597125}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.23832265}, {'id': 'https://openalex.org/C41008148', 'wikidata': 'https://www.wikidata.org/wiki/Q21198', 'display_name': 'Computer science', 'level': 0, 'score': 0.122374535}, {'id': 'https://openalex.org/C150194340', 'wikidata': 'https://www.wikidata.org/wiki/Q26972', 'display_name': 'Gene expression', 'level': 3, 'score': 0.068746984}, {'id': 'https://openalex.org/C42062724', 'wikidata': 'https://www.wikidata.org/wiki/Q1209205', 'display_name': '16S ribosomal RNA', 'level': 3, 'score': 0.0}, {'id': 'https://openalex.org/C199360897', 'wikidata': 'https://www.wikidata.org/wiki/Q9143', 'display_name': 'Programming language', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D003001', 'descriptor_name': 'Cloning, Molecular', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D005822', 'descriptor_name': 'Genetic Vectors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D000225', 'descriptor_name': 'Adenine', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D000225', 'descriptor_name': 'Adenine', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003001', 'descriptor_name': 'Cloning, Molecular', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010957', 'descriptor_name': 'Plasmids', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D010957', 'descriptor_name': 'Plasmids', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013941', 'descriptor_name': 'Thymine', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D013941', 'descriptor_name': 'Thymine', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 5, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1186/s12896-018-0456-8', 'pdf_url': 'https://bmcbiotechnol.biomedcentral.com/track/pdf/10.1186/s12896-018-0456-8', 'source': {'id': 'https://openalex.org/S53489610', 'display_name': 'BMC Biotechnology', 'issn_l': '1472-6750', 'issn': ['1472-6750'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320256', 'host_organization_name': 'BioMed Central', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310320256'], 'host_organization_lineage_names': ['Springer Nature', 'BioMed Central'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://doaj.org/article/3904a70363f14f8eb7d6aaf63981a67e', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306401280', 'display_name': 'DOAJ (DOAJ: Directory of Open Access Journals)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': None, 'host_organization_name': None, 'host_organization_lineage': [], 'host_organization_lineage_names': [], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc6045858', 'pdf_url': 'https://europepmc.org/articles/pmc6045858?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045858', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/30005664', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1186/s12896-018-0456-8', 'pdf_url': 'https://bmcbiotechnol.biomedcentral.com/track/pdf/10.1186/s12896-018-0456-8', 'source': {'id': 'https://openalex.org/S53489610', 'display_name': 'BMC Biotechnology', 'issn_l': '1472-6750', 'issn': ['1472-6750'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320256', 'host_organization_name': 'BioMed Central', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310320256'], 'host_organization_lineage_names': ['Springer Nature', 'BioMed Central'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [{'funder': 'https://openalex.org/F4320321001', 'funder_display_name': 'National Natural Science Foundation of China', 'award_id': '31660412'}], 'datasets': [], 'versions': [], 'referenced_works_count': 25, 'referenced_works': ['https://openalex.org/W17105100', 'https://openalex.org/W1971496956', 'https://openalex.org/W1974136686', 'https://openalex.org/W1985849437', 'https://openalex.org/W2011842967', 'https://openalex.org/W2012746081', 'https://openalex.org/W2021236910', 'https://openalex.org/W2025920026', 'https://openalex.org/W2036917159', 'https://openalex.org/W2041463136', 'https://openalex.org/W2048408401', 'https://openalex.org/W2050917919', 'https://openalex.org/W2069477714', 'https://openalex.org/W2078290510', 'https://openalex.org/W2082018310', 'https://openalex.org/W2088551283', 'https://openalex.org/W2090899964', 'https://openalex.org/W2096986539', 'https://openalex.org/W2109214798', 'https://openalex.org/W2121977001', 'https://openalex.org/W2122139577', 'https://openalex.org/W2132417094', 'https://openalex.org/W2163448850', 'https://openalex.org/W2517568995', 'https://openalex.org/W2581867307'], 'related_works': ['https://openalex.org/W30564900', 'https://openalex.org/W2883076366', 'https://openalex.org/W2593465343', 'https://openalex.org/W2492440923', 'https://openalex.org/W209814502', 'https://openalex.org/W2077097444', 'https://openalex.org/W2048696032', 'https://openalex.org/W2000678289', 'https://openalex.org/W1997953487', 'https://openalex.org/W17134236'], 'abstract_inverted_index': {'With': [0], 'the': [1, 53, 77, 111, 128, 162, 171, 206, 272, 283, 287], 'rapid': [2], 'development': [3], 'of': [4, 9, 136, 208, 262, 271, 289], 'sequencing': [5], 'technologies,': [6], 'increasing': [7, 134], 'amount': [8], 'genomic': [10], 'information': [11], 'has': [12, 38, 231], 'been': [13, 39], 'accumulated.': [14], 'To': [15], 'clone': [16, 213], 'genes': [17], 'for': [18, 121, 182, 316], 'further': [19], 'functional': [20], 'studies': [21], 'in': [22, 161, 239, 266], 'large': [23], 'scale,': [24], 'a': [25, 45, 58, 100, 193, 200], 'cheap,': [26], 'fast': [27], 'and': [28, 48, 70, 92, 105, 127, 170, 187, 195, 224, 246, 254, 303], 'efficient': [29], 'cloning': [30, 104, 113, 149, 164, 173, 223, 253, 279, 305, 318], 'vector': [31, 36, 43, 54, 211, 311], 'is': [32], 'desired.': [33], 'A': [34], 'bifunctional': [35], 'pXST': [37, 42, 101, 202, 210, 229], 'constructed.': [40], 'The': [41, 138, 228], 'harbors': [44], 'XcmI-ccdB-XcmI': [46, 244], 'cassette': [47, 245], 'restriction': [49, 247], 'site': [50, 248], 'SmaI.': [51], 'Digestion': [52], 'with': [55, 82, 123, 133, 220], 'XcmI': [56], 'generates': [57], 'single': [59], 'thymidine': [60], '(T)': [61], 'overhang': [62], 'at': [63], '3′': [64], 'end': [65, 74], 'which': [66], 'facilitates': [67], 'TA': [68, 103, 112, 155, 222, 252], 'cloning,': [69], 'SmaI': [71], 'gives': [72], 'blunt': [73], 'that': [75, 240], 'enables': [76], 'blunt-end': [78, 106, 119, 225, 255], 'ligation.': [79, 256], 'Multiple': [80], 'products': [81, 215], 'various': [83], 'sizes': [84], 'were': [85], 'amplified': [86, 216], 'from': [87, 217, 291], 'cassava': [88, 218], 'genome': [89, 219], 'by': [90, 281, 300], 'PCR': [91, 94, 214], 'each': [93], 'fragment': [95, 153], 'was': [96, 146], 'separately': [97], 'cloned': [98], 'into': [99], 'using': [102, 154, 209], 'ligation': [107, 120, 168, 226], 'methods.': [108, 227, 306], 'In': [109], 'general,': [110], 'gave': [114], 'higher': [115], 'transformation': [116, 129, 140], 'efficiency': [117, 130, 141, 165, 174, 280], 'than': [118], 'inserts': [122, 184], 'all': [124], 'different': [125], 'sizes,': [126], 'significantly': [131], 'decreased': [132], 'size': [135], 'inserts.': [137], 'highest': [139], '(8.6': [142], '×': [143], '106': [144], 'transformants/μg)': [145], 'achieved': [147], 'when': [148], '517': [150, 186], 'bp': [151], 'DNA': [152], 'cloning.': [156], 'No': [157], 'significant': [158], 'difference': [159], 'observed': [160], 'positive': [163, 172, 263, 278], 'between': [166], 'two': [167], 'methods': [169], 'could': [175, 297], 'reach': [176], 'as': [177, 179], 'high': [178, 301], '100%': [180], 'especially': [181], 'small': [183], '(e.g.': [185], '957': [188], 'base': [189], 'pairs).': [190], 'We': [191, 204], 'describe': [192], 'simple': [194], 'general': [196], 'method': [197], 'to': [198, 212, 320], 'construct': [199], 'novel': [201], 'vector.': [203], 'confirm': [205], 'feasibility': [207], 'both': [221, 251], 'plasmid': [230], 'several': [232], 'advantages': [233], 'over': [234], 'many': [235], 'currently': [236], 'available': [237], 'vectors': [238], '(1)': [241], 'it': [242, 258, 276, 296], 'possesses': [243], 'SmaI,': [249], 'enabling': [250], '(2)': [257], 'allows': [259], 'direct': [260], 'selection': [261], 'recombinant': [264], 'plasmids': [265], 'Escherichia': [267], 'coli': [268], 'through': [269], 'disruption': [270], 'ccdB': [273, 284], 'gene.': [274], '(3)': [275], 'improves': [277], 'introducing': [282], 'gene,': [285], 'reducing': [286], 'possibility': [288], 'self-ligation': [290], 'insufficient': [292], 'digested': [293], 'plasmids.': [294], '(4)': [295], 'be': [298], 'used': [299], 'performance': [302], 'cost-effective': [304], 'Therefore,': [307], 'this': [308], 'dual': [309], 'function': [310], 'would': [312], 'offer': [313], 'flexible': [314], 'alternatives': [315], 'gene': [317], 'experiments': [319], 'researchers.': [321]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2883076366', 'counts_by_year': [{'year': 2022, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 1}], 'updated_date': '2024-12-24T08:49:53.413320', 'created_date': '2018-08-03'}