Title: Effects of g2S subunit incorporation on GABA A receptor macroscopic kinetics
Abstract:GABAA receptors, the major inhibitory neurotransmitter receptors in the mammalian central nervous system, are heteropentameric proteins. We are interested in understanding the contribution of the g su...GABAA receptors, the major inhibitory neurotransmitter receptors in the mammalian central nervous system, are heteropentameric proteins. We are interested in understanding the contribution of the g subunit to the kinetic properties of GABAA receptors. Studies in Xenopus oocytes have suggested that co-expression of a1, b2, and g2S subunits results in the formation of both ab and abg receptors (Boileau et al., 2002a; Boileau et al., 1998). Here, we have used an excess of the g2S subunit in transfections of HEK293 cells to bias expression toward abg-containing receptors. Using rapid application and whole cell patch clamp techniques, we found that incorporation of the g subunit eliminated the rapid phases of desensitization and accelerated deactivation, consistent with a proposed role of desensitization in slowing deactivation. In addition, abg receptors had an increased GABA EC50, reduced sensitivityRead More
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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