Title: Gene Expression Stability of Housekeeping Genes in Poplar Clone 107 under Salt Stress by Real Time PCR
Abstract: Gene expression stabilities of nine housekeeping genes in Poplar Clone 107 ( Populus × euramericana ' Neva' ) under different durations of salt stress treatments and control were detected by Real-time PCR; the best reference genes were screened through the geNorm program. The results indicated that amplification efficiency of six housekeeping genes were from 89.93% to 104.68%,and fitting degree of linear regression (R 2 ) were from 0.987 6 to 0.999 9。Under the conditions of the salt stresses, the order of 6 housekeeping genes expression stabilities from high to low were EIF 4B=UBQ>CYP>ACTⅡ>UBQL>TUB,and the genes, EIF 4B and UBQ, should be suitable as reference genes. Whereas under the control condition, the order of six housekeeping genes expression stabilities were EIF 4B= UBQL>ACTⅡ>CYP>UBQ>TUB,and EIF 4B and UBQL could be used as reference genes
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot