Title: Selection of Internal Control Genes for Real-Time RT-PCR Normalization in White Birch(Betula platyphylla Suk.)
Abstract: In order to facilitate rapid and accurate gene expression analysis of multiple samples from birch,the expression stability of housekeeping genes such as genes coding micro-tube protein(Tu) and ubiquitin(UbQ) were assessed by qRT-PCR using a set of cDNAs from different birch samples including cells after different treatments and different parts of birch plants in different ages or different growing seasons.The results suggested that the Tu and UbQ genes could provided superior transcript normalization in birch cells treated with different elicitors(MeJA and SA),and birch plants in different growing seasons,while UbQ gene was the recommended reference gene for gene expression studies using cDNAs from white birch bark in defferent years.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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Cited By Count: 1
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