Title: Effect of tissue ischemia on prostatic androgen levels.
Abstract: 4643 Background: Androgen concentrations sufficient to activate the androgen receptor (AR) a have been measured within the prostate and in tumor metastases from men with castration resistant prostate cancer. Methods of tissue collection may influence androgen levels. We sought to investigate the effect of tissue ischemia on concentration of androgens in prostatic tissue. Methods: Men undergoing radical prostatectomy for clinically localized prostate cancer underwent intraoperative and post-operative prostate tissue acquisition. Seven time points were collected for each patient. The first time point was at time of prostate exposure in the operative field, the second time point was immediately after the blood supply was clamped, the third time point was immediately after prostate removal, and subsequent time points were in 30-60 min increments following prostate removal. At each time point, prostate tissue was immediately flash-frozen in liquid nitrogen. Steroids were extracted from the tissue, and levels of dihydrotestosterone (DHT) and testosterone (T) were determined by mass spectrometry. Results: Mean initial DHT and T tissue concentrations prior to ischemia were 3.64 ± 1.60 pg/mg and 0.54 ± 1.43 pg/mg, respectively. There was greater core-to-core variability in the assay at smaller biopsy weights (≤ 3.5 mg), however this did not significantly limit the data. Tissue ischemia did not have a significant effect on DHT concentrations up to 3 hrs following vascular clamping. A decrease in T was found at the initial ischemic point (prostate removal), however levels of T were at the lower assay detection limit, and no further decrease in T was seen with up to 3hrs of tissue devascularization. Conclusions: Average intraprostatic DHT and T concentrations in noncastrate men with prostate cancer are similar to those found in other studies, demonstrating that prostate needle biopsies are a reliable method of tissue acquisition for this purpose. Ischemia did not have a significant effect on intraprostatic concentrations of DHT and T in our assay. These findings have implications on future studies utilizing prostatic tissue for steroid hormonal measurements, particularly if obtained after prolonged ischemia as found following laparoscopic/robotic prostatectomy. No significant financial relationships to disclose.
Publication Year: 2010
Publication Date: 2010-05-20
Language: en
Type: article
Indexed In: ['crossref']
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